Ready-to-use BCA Protein Assay Kit R1491648-500T Aladdin
$59.00
In stock
SKU
R1491648-500T
Product Information
| Product Name | Ready-to-use BCA Protein Assay Kit |
| Specifications & Purity | BioReagent, for protein analysis, ready-to-use |
Product Description
The BCA method is one of the most widely used techniques for protein quantification. This kit, based on the
bicinchoninic acid (BCA) assay, enables rapid, stable, and sensitive measurement of protein concentrations. Its principle relies on the reduction of Cu²⁺ to Cu⁺ by peptide bonds in an alkaline environment, followed by the formation of a purple-colored complex between Cu⁺ and BCA. This complex exhibits strong absorbance at
562 nm, proportional to protein concentration. The kit includes a series of ready-to-use protein standard solutions (BSA), eliminating the need for dilution and simplifying the workflow.
Product Component List:
R1491648
Component
500T
500T×5
Storage
R1491648A
Reagent A
100 mL
100 mL×5
2-8℃
R1491648B
Reagent B
3 mL
3 mL×5
2-8℃
R1491648C
Ready-to-use BSA standard① (0 μg/mL)
1 mL
1 mL×5
-20℃
R1491648D
Ready-to-use BSA standard② (125 μg/mL)
1 mL
1 mL×5
-20℃
R1491648E
Ready-to-use BSA standard③ (250 μg/mL)
1 mL
1 mL×5
-20℃
R1491648F
Ready-to-use BSA standard④ (500 μg/mL)
1 mL
1 mL×5
-20℃
R1491648G
Ready-to-use BSA standard⑤ (750 μg/mL)
1 mL
1 mL×5
-20℃
R1491648H
Ready-to-use BSA standard⑥ (1000 μg/mL)
1 mL
1 mL×5
-20℃
R1491648I
Ready-to-use BSA standard⑦ (1500 μg/mL)
1 mL
1 mL×5
-20℃
R1491648J
Ready-to-use BSA standard⑧ (2000 μg/mL)
1 mL
1 mL×5
-20℃
Key Features
Convenient: Ready-to-use standards avoid tedious dilution steps.
High Accuracy: Lower coefficient of variation than Coomassie Brilliant Blue assays.
Broad Linear Range: Detection range:
20–2000 μg/mL.
Excellent Compatibility: Tolerates metal ions, reducing agents, chelators, and detergents.
Protocol (Microplate Reader Example)
Prepare Working Reagent:
a. Calculate the total volume required:
Working reagent volume = (Number of standard samples + Number of test samples) × Replicates × Volume per well
Example: 8 standards + 3 test samples with 3 replicates each at 200 μL/well:
(8 + 3) × 3 × 200 μL =
6.6 mL
b. Mix
Reagent A
and
Reagent B
at a
50:1
ratio to prepare the working reagent. Vortex thoroughly.
Note:
Prepare extra volume (1–2 wells) to compensate for pipetting errors.
Freshly prepared working reagent is stable for
24 hours
at room temperature (sealed).
Quantification Steps:
① Add 20 μL of each ready-to-use BSA standard (①–⑧) to a 96-well plate (mix thoroughly before use):
Well
1
2
3
4
5
6
7
8
Standard
①
②
③
④
⑤
⑥
⑦
⑧
Volume (μL)
20
20
20
20
20
20
20
20
BSA (μg/mL)
0
125
250
500
750
1000
1500
2000
② Dilute test samples in
1× PBS or 0.9% saline
(e.g., 2×, 4×, 8× serial dilutions). Add
20 μL
to wells.
③ Add
200 μL
working reagent to each well. Mix thoroughly, cover the plate, and incubate at
37°C for 30 min. Cool to room temperature.
Note: Incubation alternatives:
Room temperature: 2 hours
60°C: 30 minutes
Absorbance increases with time/temperature. Extend incubation for low-concentration samples.
④ Measure absorbance at
562 nm
(or 540–590 nm) using a microplate reader. Subtract the blank control (Standard ① + working reagent) absorbance.
⑤ Plot a standard curve and calculate sample protein concentrations.
Note:
Exclude obvious outliers.
Multiply calculated concentrations by dilution factors.
Use linear regression for computer-based fitting.
Precautions
Compatible with
microplate readers
(microplate method) or
spectrophotometers
(cuvette method). For cuvettes, ensure the volume meets the minimum requirement (may reduce total number of assays).
If precipitates form during cold/long-term storage, stir or warm to
37°C
to dissolve.
Always generate a fresh standard curve
for each assay to ensure accuracy.
For samples with high levels of interfering substances (see Appendix), consider alternative protein quantification methods.
Wear a lab coat and disposable gloves during operations.
For research use only.
Storage and Shipping
| Shipped In | Ice chest + Ice pads |
| Stability and Storage | Each component has a shelf life of 1 year under corresponding storage conditions. |
| Storage Temp | Store at 2-8°C,Store at -20°C |
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