A549 Whole Cell Lysate cl155906-200μg Aladdin
$518.00
In stock
SKU
cl155906-200μg
| Catalog No. | Size | Price (USD) |
| cl155906-200μg | 200μg | $518.90 |
Product Information
| Product Name | A549 Whole Cell Lysate |
| Short Description | Mycoplasma free |
| Form | Lyophilized |
| Specifications & Purity | Carrier Free, Azide Free, 200μg, Mycoplasma free |
Product Description
The A549 whole cell was prepared using a standard whole cell lysate protocol. The concentration was determined using the BCA assay process.
A549 cells are used as an in vitro model for a type II pulmonary epithelial cell model for drug metabolism. Cells are cytokeratin positive by immunoperoxidase staining. This line was initiated in 1972 by D.J. Giard, et al. through explant culture of lung carcinomatous tissue from a 58-year-old Caucasian male (PubMed 4357758). Further studies by M. Lieber, et al. revealed that A549 cells could synthesize lecithin with a high percentage of desaturated fatty acids utilizing the cytidine diphosphocholine pathway (PubMed 175022).
Reconstitution
Recommended reconstitute at 1-2 mg/mL in ddH2O. Use at an assay dependent concentration. Ready to load on SDS-PAGE for Western blotting, 20 µg per lane is recommended for mini gel. Lyophilized contents may appear as either a translucent film or a white power. This variance does not affect the quality of the product. Lyophilized contents may appear as either a translucent film or a white powder. This variance does not affect the quality of the product.
Storage and Shipping
| Shipped In | Dry ice packs + Cold packs |
| Stability and Storage | Shipped on Dry Ice. Lyophilizate is recommended stored at -80℃. After reconstitution, recommended aliquot and avoid repeated freeze-thaw, store at -20℃ for short-term storage or -80℃ for long-term storage. |
| Storage Temp | Store at -80°C |
Additional Information
| Application | WB |
| Concentration | 200μg, Mycoplasma free |
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