o-Phenylenediamine(OPD), CAS No.95-54-5 O755484-50g Aladdin
| Catalog No. | Size | Price (USD) |
| o755484-25g | 25g | $109.90 |
| o755484-100g | 100g | $705.90 |
| Catalog No. | Size | Price (USD) |
| o755484-25g | 25g | $109.90 |
| o755484-100g | 100g | $705.90 |
Product Information
| Product Name | o-Phenylenediamine(OPD), CAS No.95-54-5 |
| CAS | 95-54-5 |
| Specifications & Purity | ≥98%, Peroxidase substrate |
Product Description
o
-Phenylenediamine (OPD) Free Base is a peroxidase substrate suitable for use in enzyme linked immunosorbent assay (ELISA) procedures. The substrate produces a soluble product that is orange-brown in color and can be read spectrophotometrically at 450 nm. The OPD reaction may be stopped with 3N HCl or 3M H
2
SO
4
and read at 492 nm.
Application:
o
-Phenylenediamine has been used in the microtiter?based assay for hyaluronan quantification.
It has also been used in enzyme linked immunosorbent assay (ELISA) for color development.
Storage and Shipping
| Shipped In | Wet ice |
| Storage Temp | Store at 2-8°C |
Additional Information
| China Danger Code | w |
| EC Number | 202-430-6 |
| MDL Number | MFCD00007721 |
| Molecular Formula | C6H8N2 |
| Pubchem CID | 7243 |
| Solubility | ethanol: 50 mg/mL, clear, colorless to faintly yellow |
| Concentration | Peroxidase substrate |
1. Xuyuan Sun, Minmin Liu, Hao Liu, Li Li, Yaping Ding. (2024) A molecularly imprinted electrochemical aptasensor-based dual recognition elements for selective detection of dexamethasone. TALANTA, [PMID:38879945] [10.1016/j.talanta.2024.126404]
2. Wanjin Huang, Shufei Tang, Wei Xiao, Yafei Chen, Lijun Li, Jianping Li. (2024) A molecularly imprinted photoelectrochemical sensor based on an rGO/MoSSe heterojunction for the detection of chlortetracycline. ANALYST, [PMID:38404152] [10.1039/D4AN00172A]
3. Lijun Wang, Jiangchuan Tang, Shanshan Ren, Haixia Hu, Xiao Yang, Xianggui Chen. (2025) A novel dual-mode colorimetric and ratiometric fluorescence method for detecting isocarbophos based on S, N-CDs inhibiting Fe3O4@Cu-BTC nanozyme activity. TALANTA, [PMID:40020615] [10.1016/j.talanta.2025.127810]
4. Xuhui Xia, Peng Zhao, Jia Zheng, Xuheng Li, Xin Zeng, Dianhui Men, Yiyao Luo, Changjun Hou, Danqun Huo. (2025) A novel quantum dot-based ratiometric fluorescence sensor array: For reducing substances detection and Baijiu quality discrimination. ANALYTICA CHIMICA ACTA, [PMID:40024655] [10.1016/j.aca.2025.343785]
5. Siying Wu, Xuehua Zhang, Wenyan Tang, Yue Zhang, Xin Lv, Yu-Ting Zhuang. (2024) A ratiometric fluorescence platform based on bifunctional MOFs for sensitive detection of organophosphorus pesticides. JOURNAL OF FOOD COMPOSITION AND ANALYSIS, [PMID:] [10.1016/j.jfca.2024.106971]
6. Zihan Wang, Yihong Chen, Zexian Chen, Jiarong Duan, Huiping Wang, Erhua Guo, Jianhui Zhi, Shaomin Shuang, Chuan Dong, Jun Li, Xiaojuan Gong. (2025) A trinity sensing platform for uric acid detection in clinical samples using Fe-functionalized carbon quantum dot nanozymes. TALANTA, [PMID:40164024] [10.1016/j.talanta.2025.128007]
7. Wenpeng Jiang, Wenli Zhu, Qingli Yang, Wei Wu, Xiudan Hou. (2025) An electrochemical/colorimetric dual-mode sensor for the detection of AFB1 in agricultural products based on CoFe2O4@Au nanoparticles and smartphone imaging. FOOD CHEMISTRY, [PMID:39923524] [10.1016/j.foodchem.2025.143201]
8. Yinnan Jing, Jiamin Wang, Xuan Liu, Shurong Li, Qinkai Yang, Mengnan Wang, Xueyi Fan, Guangsheng Ge, Kai Zheng, Jianlong Wang, Xiaoqian Tang, Qi Zhang, Yanru Wang. (2025) An Enhanced Ratiometric Fluorescence Immunosensor for Rapid Detection of AFB1 Based on the Dual-Enzyme Synergistic Co-Catalytic Effect of Natural Enzymes and Nanozymes. JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY, [PMID:40131343] [10.1021/acs.jafc.5c01127]
9. Shao Huikai, Zhao Lingguo, Liu Zhenping, Li Jinqi. (2024) An epitope imprinted electrochemical sensor for highly sensitive and selective determination of prostate-specific antigen. MICROCHIMICA ACTA, 191 (11): (1-11). [PMID:39387910] [10.1007/s00604-024-06737-y]
10. Lijun Yang, Zhiyi Zhang, Tiantian Zhou, Wei Qi, Mengfan Wang. (2025) An oxidase-like nanozyme-based sensor array for the specific detection and discrimination of catechins. Analytical Methods, [PMID:40094452] [10.1039/D4AY02133A]
11. Si Haibin, Du Dexin, Jiao Chengcheng, Sun Yan, Li Lu, Tang Bo. (2024) Biomimetic synergistic effect of redox site and Lewis acid for construction of efficient artificial enzyme. Nature Communications, 15 (1): (1-16). [PMID:39060279] [10.1038/s41467-024-50687-1]
12. Ding Shurong, Liu Shasha, Yang Yuzhuo, Shi Linlin, Zhang Yongqiang, Yu Jingkun, Song Haoqiang, Shi Yingge, Lu Siyu. (2025) Carbon dots self-assembly enables near-infrared emission. Science China-Chemistry, [PMID:] [10.1007/s11426-024-2308-3]
13. Xi Wang, Guiquan Liu, Xueyu Pu, Tangjun Ren, Fan Zhang, MengJie Shen, Yan Zhu, Alexander Kros, Jian Yang. (2025) Combating cisplatin-resistant lung cancer using a coiled-coil lipopeptides modified membrane fused drug delivery system. JOURNAL OF CONTROLLED RELEASE, [PMID:39756686] [10.1016/j.jconrel.2025.01.004]
14. Zhen Chen, Yan Wang, Zebing Ning, Qi Huang, Jia Zhou, Faquan Yu. (2024) Conjugated Microporous Polymer/TiS2 Composite Cathode for High-Performance Lithium-Rechargeable Batteries. ACS Applied Polymer Materials, [PMID:] [10.1021/acsapm.4c02203]
15. Binbin Zhou, Hao Xie, Xinyi Li, Yongbo Zhu, Lijun Huang, Ming Zhong, Liang Chen. (2024) Construction of a self–reporting molecularly–imprinted electrochemical sensor based on CuHCF modified by rGNR–rGO for the detection of zearalenone. FOOD CHEMISTRY, [PMID:38555687] [10.1016/j.foodchem.2024.139154]
16. Meng Zhou, Miaomiao Chen, Yunyun Wang, Caihong Mao, Yu Zhang, Junmei Liu, Xikun Zhang, Shujie Pang, Xudong Yang. (2025) Construction of carbon dot-embedded fluorescent hydrogels based on oxidized gum arabic-gelatin Schiff base for Cr(VI) detection applications. INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES, [PMID:39761895] [10.1016/j.ijbiomac.2025.139466]
17. Yuqing Huang, Yulong Zhu, Chao Li, Guangsong Zheng, Chenxi Zhang, Yang Nan, Qing Lou, Xu Chen, Yongsheng Zhu, Jinyang Zhu. (2024) Construction of photoluminescence-afterglow dual-mode white emission materials from carbon dots via Förster resonance energy transfer. Journal of Materials Chemistry C, 12 (36): (14246-14252). [PMID:] [10.1039/D4TC01765J]
18. Shao-Hua Wen, Ning Wang, Ziqi Yuan, Hao Wu, Gege Yan, Hengyuan Zhang, Sha Yu, Junping Ma, Yuanzhen Zhou. (2025) Detection of arsenate in colored grains using an interference-free dual-signal ratiometric HEC sensor. FOOD CHEMISTRY, [PMID:39938269] [10.1016/j.foodchem.2025.143290]
19. Xin-He Duan, Hong-Wei Li, Yi Li, Zhijiu Huang, Sha Liu, Yuqing Wu. (2024) Determination of acetaldehyde in Chinese Baijiu based on the light-activated oxidase-mimic activity of fluorine-containing carbon dots (dF-CDs). SENSORS AND ACTUATORS B-CHEMICAL, [PMID:] [10.1016/j.snb.2024.136356]
20. Chengchen Tan, Xiaolong Yan, Xingchang Lu, Jianxiu Wang, Xinyao Yi. (2024) Dual-mode colorimetric and fluorescent detection of BRCA1 based on a CRISPR-Cas12a system. ANALYST, [PMID:39171896] [10.1039/D4AN01035C]