Product Information
| Product Name | Myc tag Mouse mAb |
| Short Description | mAb (7D9-4C6-6F10); Mouse anti Myc tag Antibody; WB, ICC, IF, IP; Unconjugated |
| Form | Liquid |
| Specifications & Purity | ExactAb™, Validated, High performance, 1.0 mg/mL |
| SDS Page | 150 kDa |
| Synonyms | c-myc tag antibody | Myc Epitope Tag antibody | avian myelocytomatosis viral oncogene homolog | BHLHE39 | bHLHe39MRTL | Class E basic helix-loop-helix protein 39 | cMyc | c-Myc | myc proto-oncogene protein | Myc | Myc2 | MYCC | myc-related translation/loc |
Product Description
Mouse anti Myc tag Antibody, Monoclonal (7D9-4C6-6F10), could be used for WB, ICC, IF, IP and so on.
Application:
WB: 1/1000 - 1/10000
IF/ICC: 1/100 - 1/500
IP: 1/200
Relevance
Epitope tags are short peptide sequences that are easily recognized by tag-specific antibodies. Due to their small size, epitope tags do not affect the tagged protein鈥檚 biochemical properties. Most often sequences encoding the epitope tag are included with target DNA at the time of cloning to produce fusion proteins containing the epitope tag sequence. This allows anti-epitope tag antibodies to serve as universal detection reagents for any tag containing protein produced by recombinant means. This means that anti-epitope tag antibodies are a useful alternative to generating specific antibodies to identify, immunoprecipitate or immunoaffinity purify a recombinant protein. The anti-epitope tag antibody is usually functional in a variety of antibody-dependent experimental procedures.
Expression vectors producing epitope tag fusion proteins are available for a variety of host expression systems including bacteria, yeast, insect and mammalian cells.
Storage and Shipping
| Shipped In | Ice chest + Ice pads |
| Stability and Storage | Store at 4°C short term (1-2 weeks). Store at -20°C long term (24 months). Upon receipt, it is recommended to aliquot. Avoid freeze/thaw cycle. |
| Storage Temp | Store at -20°C,Avoid repeated freezing and thawing |
Additional Information
| Clone | 7D9-4C6-6F10 |
| Immunogen | A synthetic peptide derived from Myc-Tag (EQKLISEEDL). |
| Isotype | Mouse IgG1 |
| Positive Control | WB: 11654-myc lysates. ICC/IF: 293 cells transfected with a Myc tag protein. IP: Transfected 293 cell lysates with c-Myc tag fusion protein |
| Purification Method | Protein G purified |
| Specificity | Myc |
| Concentration | 1.0 mg/mL |