| Catalog No. | Size | Price (USD) |
| rp163946-gmp-25μg | 25μg | $709.90 |
| rp163946-gmp-100μg | 100μg | $1,659.90 |
| rp163946-gmp-500μg | 500μg | $4,979.90 |
Product Information
| Product Name | Recombinant Human Sonic Hedgehog/Shh (C24II) GMP Protein |
| Short Description | GMP, ≥95% (SDS-PAGE&SEC-HPLC), Active, E. coli, No tag, 24-197 aa (C24II) |
| Expressed Host | E. coli |
| Accession | NP_000184.1 |
| Amino Acids | 24-197 aa (C24II) |
| Protein TAG | No tag |
| Specifications & Purity | Animal Free,Carrier Free,Bioactive,ActiBioPure™,High Performance,PBS Only,≥95%(SDS-PAGE&SEC-HPLC) |
| Endotoxin Concentration | <0.01 EU/μg |
| Biological Activity | Measured by its ability to induce alkaline phosphatase production by C3H10T1/2 mouse embryonic fibroblast cells. The ED50 for this effect is 0.1-0.4 µg/mL. |
| SDS Page | 22 kDa, under reducing conditions |
| Sequence | MIIGPGRGFGKRRHPKKLTPLAYKQFIPNVAEKTLGASGRYEGKISRNSERFKELTPNYNPDIIFKDEENTGADRLMTQRCKDKLNALAISVMNQWPGVKLRVTEGWDEDGHHSEESLHYEGRAVDITTSDRDRSKYGMLARLAVEAGFDWVYYESKAHIHCSVKAENSVAAKSGG |
| Synonyms | HHG1 | HHG-1 | HLP3 | HPE3 | MCOPCB5 | MCOPCB5sonic hedgehog (Drosophila) homolog | Shh | ShhNC | SMMCI | SMMCIsonic hedgehog homolog (Drosophila) | sonic hedgehog homolog | sonic hedgehog protein | Sonic Hedgehog | TPT | TPTPS |
Reconstitution
Reconstitute at 100-200 μg/mL in PBS, and allow up to 24 hours at 2 to 8 °C for complete reconstitution.
Storage and Shipping
| Shipped In | Ice chest + Ice pads |
| Stability and Storage | A minimum of 12 months when stored at ≤ -20 ℃ as supplied. 1 month, 2 to 8 ℃ under sterile conditions after reconstitution. 3 months, ≤ -28 ℃ under sterile conditions after reconstitution. Upon receipt, it is recommended to aliquot. Avoid freeze/thaw cycl |
| Storage Temp | Store at -20°C,Avoid repeated freezing and thawing |
Additional Information
| N Terminal Sequence | Met-Ile-Ile-Gly25-Pro-Gly-Arg-Gly-Phe-Gly |
MDSD
