Deoxyribonuclease I, CAS No.9003-98-9 D128589-2KU Aladdin
$382.00
In stock
SKU
D128589-2KU
Product Information
| Product Name | Deoxyribonuclease I, CAS No.9003-98-9 |
| CAS | 9003-98-9 |
| Specifications & Purity | EnzymoPure™, Recombinant, ≥2 units/ul;from Pichia pastoris;Solution |
Product Description
description：
Bovine pancreatic deoxyribonuclease I produced recombinantly in yeast, Pichia pastoris, to decrease levels of contaminating RNase and eliminate potential pathogens associated with animal based materials.
Bovine pancreas is a rich source of RNase A which is often found in many commercial DNase preparations. Producing DNase I by recombinant means in an organism with much lower levels of endogenous RNase greatly facilitates purification of an enzyme with undetectable levels of RNase. The processes involved in the production and isolation of recombinant DNase I are completely devoid of animal based components which eliminates the possibility of introducing animal derived pathogens into bioprocessing procedures.
Animal Free/AF. Recombinant Bovine pancreatic deoxyribonuclease 1 produced in Pichia pastoris. Chromatographically purified. Free of animal derived components, RNases & proteases. A liquid preparation in 5mM Calcium Acetate, 4mg/ml glycine, pH 5.0 and 50% glycerol. Supplied with 10x reaction buffer.
Storage Buffer: 5mM calcium acetate, 4mg/ml glycine, pH 5.0 and 50% glycerol.
DNase I Reaction Buffer (10X): 500mM Tris-HCl, 10mM MgSO4, 1mM CaCl2, pH 7.8, provided.
application：
Recombinant DNase I is suitable for such applications as:
• Removing genomic DNA from RNA preparations prior to RT-PCR
• Degradation of DNA templates after transcription reactions
• Removing unwanted DNA from samples prior to Northern blotting
• Removing DNA during biopharma and bioprocessing procedures
Storage and Shipping
| Shipped In | Ice chest + Ice pads |
| Storage Temp | Store at -20°C |
Additional Information
| EC Number | 232-667-0 |
| MDL Number | MFCD00130918 |
| Pubchem CID | 597667 |
| Unit Definition | One Unit causes an increase in absorbance at 260nm of 0.001 per minute at 25℃ when acting upon highly polymerized DNA at pH 5.0. |
| Concentration | ≥2 units/ul;from Pichia pastoris;Solution |
1. Shaopeng Liu, Dan Peng, Shuohan He, Xuan Li, Yi Wu, Xin Liu, Yang Zhang, Peng Liu, Kaiyong Cai. (2023) Ternary low-temperature phototherapy nano-systems for the treatment of diabetic wounds. Journal of Materials Chemistry B, 12 (1): (264-274). [PMID:38088036] [10.1039/D3TB02224B]
2. Chenfei Han, Yu Xiao, Zhenjiang Liu, Daolin Du, Ming Li. (2023) Cascade amplifying aptasensor for positively correlated detecting OTA: Based on DNase I-assisted cyclic enzyme digestion and AgNPs@gel-enhanced fluorescence. FOOD CONTROL, [PMID:] [10.1016/j.foodcont.2023.109970]
3. Ningning Lei, Xu Peng, Mengyue Hu, Chang Wan, Xixun Yu. (2023) Research on essential performance of oxidized chitosan-crosslinked acellular porcine aorta modified with bioactive SCPP/DOPA for esophageal scaffold with enhanced mechanical strength, biocompatibility and anti-inflammatory. INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES, [PMID:37100332] [10.1016/j.ijbiomac.2023.124522]
4. Hui-Zhen Zhang, Mei Shu, Wen-Yu Yang, Hong Pan, Men-Xuan Tang, Yuan-Yang Zhao, Chan Zhong, Guo-Ping Wu. (2022) Isolation and characterization of a novel Salmonella bacteriophage JNwz02 capable of lysing Escherichia coli O157:H7 and its antibacterial application in foods. LWT-FOOD SCIENCE AND TECHNOLOGY, [PMID:] [10.1016/j.lwt.2022.114251]
5. Luan Zhaohui, Liu Shuang, Wang Wei, Xu Kaige, Ye Shaosong, Dan Ruijue, Zhang Hong, Shu Zhenzhen, Wang Tongchuan, Fan Chaoqiang, Xing Malcolm, Yang Shiming. (2022) Aligned nanofibrous collagen membranes from fish swim bladder as a tough and acid-resistant suture for pH-regulated stomach perforation and tendon rupture. Biomaterials Research, 26 (1): (1-20). [PMID:36348451] [10.1186/s40824-022-00306-1]
6. Can Cheng, Xu Peng, Linjie Xi, Yihao Luo, Yuhang Wang, Yufan Zhou, Xixun Yu. (2022) Feasibility study of oxidized naringin as a novel crosslinking agent for crosslinking decellularized porcine Achilles tendon and its potential application for anterior cruciate ligament repair. JOURNAL OF BIOMEDICAL MATERIALS RESEARCH PART A, 111 (2): (170-184). [PMID:36054309] [10.1002/jbm.a.37440]
7. Guangquan Hu, Xiaojie Ding, Feng Gao, Jiehua Li. (2022) Calcium and integrin binding protein 1 (CIB1) induces myocardial fibrosis in myocardial infarction via regulating the PI3K/Akt pathway. EXPERIMENTAL ANIMALS, [PMID:34349085] [10.1538/expanim.21-0063]
8. Jiansheng Liu, Xueqin Qing, Qin Zhang, Ningyue Yu, Mengbin Ding, Zhaohui Li, Zhen Zhao, Zhiling Zhou, Jingchao Li. (2021) Oxygen-producing proenzyme hydrogels for photodynamic-mediated metastasis-inhibiting combinational therapy. Journal of Materials Chemistry B, 9 (26): (5255-5263). [PMID:34138994] [10.1039/D1TB01009C]
9. Yu Luo, Shenyu Huang, Lie Ma. (2021) A novel detergent-based decellularization combined with carbodiimide crosslinking for improving anti-calcification of bioprosthetic heart valve. Biomedical Materials, 16 (4): (045022). [PMID:33979785] [10.1088/1748-605X/ac0088]
10. Yu Luo, Lie Ma. (2020) Bioprosthetic heart valves with reduced immunogenic residuals using vacuum-assisted decellularization treatment. Biomedical Materials, 15 (6): (065012). [PMID:33016260] [10.1088/1748-605X/abaabf]
11. Yu Luo, Dong Lou, Lie Ma, Changyou Gao. (2019) Optimizing detergent concentration and processing time to balance the decellularization efficiency and properties of bioprosthetic heart valves. JOURNAL OF BIOMEDICAL MATERIALS RESEARCH PART A, 107 (10): (2235-2243). [PMID:31125175] [10.1002/jbm.a.36732]
12. Xuancheng Zhang, Zhaoyi Fang, Eunshinae Cho, Kai Huang, Jinzhong Zhao, Jia Jiang, Xiaoqiao Huangfu. (2019) Use of a Novel, Reinforced, Low Immunogenic, Porcine Small Intestine Submucosa Patch to Repair a Supraspinatus Tendon Defect in a Rabbit Model. Biomed Research International, [PMID:31073531] [10.1155/2019/9346567]
13. Ma Liang, Guo Ting, Pan Shuli, Zhang Yuhao. (2018) A fluorometric aptasensor for patulin based on the use of magnetized graphene oxide and DNase I-assisted target recycling amplification. MICROCHIMICA ACTA, 185 (10): (1-6). [PMID:30276550] [10.1007/s00604-018-3023-z]
14. Yuanting Xu, Chengcheng Huang, Li Li, Xixun Yu, Xu Wang, Hong Peng, Zhipeng Gu, Yaping Wang. (2013) In vitro enzymatic degradation of a biological tissue fixed by alginate dialdehyde. CARBOHYDRATE POLYMERS, [PMID:23618251] [10.1016/j.carbpol.2013.03.021]
15. Yuanting Xu, Li Li, Hao Wang, Xixun Yu, Zhipeng Gu, Chengcheng Huang, Hong Peng. (2012) In vitro cytocompatibility evaluation of alginate dialdehyde for biological tissue fixation. CARBOHYDRATE POLYMERS, [PMID:23218319] [10.1016/j.carbpol.2012.09.096]
16. Zongyou Jiang, Zhongzuo Huang, Yating Li, Xiaoxiao Yu, Yannan Ma, Jinyan Yu, Yifan Xiang, Yue Lv, Shaoqi Gao, Yicheng Luo, Bo Wang. (2024) A vascularized adipose organoid model using stromal vascular fraction cells from ruminant animals. STAR Protocols, [PMID:38635394] [10.1016/j.xpro.2024.103019]
17. Jingyao Li, Huixi Yi, Yuanyuan Fu, Jiani Zhuang, Zhixiong Zhan, Liyou Guo, Ji Zheng, Xiyong Yu, Dong-Yang Zhang. (2024) Biodegradable iridium coordinated nanodrugs potentiate photodynamic therapy and immunotherapy of lung cancer. JOURNAL OF COLLOID AND INTERFACE SCIENCE, [PMID:39488900] [10.1016/j.jcis.2024.10.156]
18. Zhoujiang Chen, Lianlin Long, Ji Wang, Mingli Jiang, Wei Li, Wenguo Cui, Liang Zou. (2024) Enhanced Tumor Site Accumulation and Therapeutic Efficacy of Extracellular Matrix-Drug Conjugates Targeting Tumor Cells. Small, [PMID:38829027] [10.1002/smll.202402040]
19. Jingnan Li, Chengyan Luo, Tingyu Sun, Yinglin Zhou, Xinchang Huang, Dezhou Wu, Xirui Luo, Chao Zeng, Huanan Li. (2024) Hypoxia-Specific Metal–Organic Frameworks Augment Cancer Immunotherapy of High-Intensity Focused Ultrasound. ACS Nano, [PMID:38949962] [10.1021/acsnano.4c02921]
20. yuke cen, Lin Zhang, Yue Jiang, Xiang-Fu Meng, Yuan Li, Chao Xiang, Ya-Ping Xue, Yu-Guo Zheng. (2024) Not exclusively the activity, but the sweet spot: a dehydrogenase point mutation synergistically boosts activity, substrate tolerance, thermo-stability and yield. ORGANIC & BIOMOLECULAR CHEMISTRY, [PMID:38529785] [10.1039/D4OB00211C]
Reviews
Write Your Own Review