Urease (URH), CAS No.9002-13-5 U1492281-250U Aladdin
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U1492281-250U
Product Information
| Product Name | Urease (URH), CAS No.9002-13-5 |
| Accession | Q03282,Q03283,Q03284 |
| CAS | 9002-13-5 |
| Specifications & Purity | EnzymoPure™, ActiBioPure™, Recombinant, Bioactive, High Performance, >150 U/mg protein; expressed in E.coli |
| Biological Activity | >150 U/mg protein |
| Synonyms | ureA | ureB | ureC |
Product Description
Ureases, functionally, belong to the superfamily of amidohydrolases and phosphotriesterases. It is an enzyme that catalyzes the hydrolysis of urea into carbon dioxide and ammonia.
Reaction Equation
Specification
Specific Activity: >150 U/mg protein
Contaminants:
NADPH oxidase: <0.001%
Properties
pH stability: pH 8.0-9.5 (37°C, 1 weeks)
Thermal stability: ≤ 65°C (pH 8.0, 10 min.)
Optimum pH: 6.0
Optimum temp.: ≥37°C
Km value: 1.94× 10⁻⁵ mmol/L (Urea)
Molecular weight: 60.3 kDa α subunit, 11.7 kDa β subunit, 11.1 kDa λ subunit (SDS-PAGE)
Assay Procedure
I Spectrophotometric Method
Wavelength: 340 nm, Light path length: 1 cm
Temperature: 25°C
Pipette the following reagents into a cuvette:
3.00 mL: Triethanolamine-HCl buffer (0.1 mol/L, pH 7.0) containing Urea (1 mol/L)、α-Ketoglutarate (5 mmol/L)、NADPH (0.24 mmol/L)、GIDH (20 U/mL)
0.02 mL: rUrease (approx. 1.5 U/mL)
II Calculation
ΔA/min: The change in absorbance at 340 nm per minute
V: Total volume of reaction mixture (3.02 mL)
D: Enzyme dilution factor
6.2: mmol/L extinction coefficient of NADPH (L⋅mmol −1⋅cm −1)
d: Light path length (1 cm)
v: Volume of enzyme sample (0.02 mL)
Reference Data
Storage and Shipping
| Shipped In | Ice chest + Ice pads |
| Stability and Storage | Store at -20℃ long term.Upon reconstitution, it is recommended to aliquot. Avoid freeze/thaw cycle. |
| Storage Temp | Store at -20°C,Avoid repeated freezing and thawing |
Additional Information
| EC Number | 232-656-0 |
| Concentration | >150 U/mg protein; expressed in E.coli |
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