Human FGF-1/FGF-acidic ELISA Kit H1509780-96T Aladdin
$599.00
In stock
SKU
H1509780-96T
Product Information
| Product Name | Human FGF-1/FGF-acidic ELISA Kit |
| Specifications & Purity | BioReagent |
Product Description
This kit employs a sandwich enzyme-linked immunosorbent assay (ELISA) for the quantitative detection of Human FGF-1/FGF-acidic concentration in samples. The Human FGF-1/FGF-acidic capture antibody is pre-coated onto the microtiter plate. Upon addition of samples or standards, Human FGF-1/FGF-acidic present therein binds to the immobilized capture antibody, while other unbound components are removed through washing. Subsequently, a biotin-conjugated detection antibody specific for Human FGF-1/FGF-acidic is added. This antibody binds to the captured Human FGF-1/FGF-acidic, forming a "sandwich" immunocomplex. Any excess, unbound material is again removed by washing. Next, an enzyme conjugate (typically Streptavidin-Horseradish Peroxidase, SA-HRP) is added.
The biotin on the detection antibody and streptavidin on the enzyme conjugate exhibit high-affinity binding, thereby linking the HRP enzyme to the immunocomplex. Following another wash step to remove unbound conjugate, a colorimetric substrate (e.g., TMB) is added. If Human FGF-1/FGF-acidic is present in the sample, the HRP linked to the complex catalyzes the oxidation of the colorless substrate, yielding a blue product. The reaction is then stopped by adding a stop solution (usually an acid), which changes the color from blue to yellow. The optical density (OD) of each well is measured at 450 nm using a microplate reader. The concentration ofHuman FGF-1/FGF-acidic is directly proportional to the OD450 value.
A standard curve is generated by assaying known concentrations ofHuman FGF-1/FGF-acidic, and the concentration of Human FGF-1/FGF-acidic in unknown samples is interpolated from this curve based on their OD values.
Storage and Shipping
| Shipped In | Wet ice |
| Stability and Storage | Store at 2-8℃ for 6 months. |
| Storage Temp | Store at 2-8°C |
Additional Information
| Detected Species Object | Human |
| Detection Range | 31.25 pg/mL~2,000 pg/mL |
| Method OF Detection | Sandwich ELISA |
| Specificity | Detecting Human FGF-1/FGF-acidic in samples, with no significant cross-reactivity to other homologs. |
Reviews
Write Your Own Review