Rat NGAL ELISA Kit R1509832-96T Aladdin
$599.00
In stock
SKU
R1509832-96T
Product Information
| Product Name | Rat NGAL ELISA Kit |
| Specifications & Purity | BioReagent |
Product Description
This kit employs a sandwich enzyme-linked immunosorbent assay (ELISA) for the quantitative detection of Rat NGAL concentration in samples. The Rat NGAL capture antibody is pre-coated onto the microtiter plate. Upon addition of samples or standards, Rat NGAL present therein binds to the immobilized capture antibody, while other unbound components are removed through washing. Subsequently, a biotin-conjugated detection antibody specific for Rat NGAL is added. This antibody binds to the captured Rat NGAL, forming a "sandwich" immunocomplex. Any excess, unbound material is again removed by washing. Next, an enzyme conjugate (typically Streptavidin-Horseradish Peroxidase, SA-HRP) is added. The biotin on the detection antibody and streptavidin on the enzyme conjugate exhibit high-affinity binding, thereby linking the HRP enzyme to the immunocomplex.
Following another wash step to remove unbound conjugate, a colorimetric substrate (e.g., TMB) is added. If Rat NGAL is present in the sample, the HRP linked to the complex catalyzes the oxidation of the colorless substrate, yielding a blue product. The reaction is then stopped by adding a stop solution (usually an acid), which changes the color from blue to yellow. The optical density (OD) of each well is measured at 450 nm using a microplate reader. The concentration ofRat NGAL is directly proportional to the OD450 value. A standard curve is generated by assaying known concentrations ofRat NGAL, and the concentration of Rat NGAL in unknown samples is interpolated from this curve based on their OD values.
Storage and Shipping
| Shipped In | Wet ice |
| Stability and Storage | Store at 2-8℃ for 6 months. |
| Storage Temp | Store at 2-8°C |
Additional Information
| Detected Species Object | Rat |
| Detection Range | 0.078 ng/mL~5 ng/mL |
| Method OF Detection | Sandwich ELISA |
| Specificity | Detecting Rat NGAL in samples, with no significant cross-reactivity to other homologs. |
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