Mouse ACE ELISA Kit M1509836-96T Aladdin
$599.00
In stock
SKU
M1509836-96T
Product Information
| Product Name | Mouse ACE ELISA Kit |
| Specifications & Purity | BioReagent |
Product Description
This kit employs a sandwich enzyme-linked immunosorbent assay (ELISA) for the quantitative detection of Mouse ACE concentration in samples. The Mouse ACE capture antibody is pre-coated onto the microtiter plate. Upon addition of samples or standards, Mouse ACE present therein binds to the immobilized capture antibody, while other unbound components are removed through washing. Subsequently, a biotin-conjugated detection antibody specific for Mouse ACE is added. This antibody binds to the captured Mouse ACE, forming a "sandwich" immunocomplex. Any excess, unbound material is again removed by washing. Next, an enzyme conjugate (typically Streptavidin-Horseradish Peroxidase, SA-HRP) is added. The biotin on the detection antibody and streptavidin on the enzyme conjugate exhibit high-affinity binding, thereby linking the HRP enzyme to the immunocomplex.
Following another wash step to remove unbound conjugate, a colorimetric substrate (e.g., TMB) is added. If Mouse ACE is present in the sample, the HRP linked to the complex catalyzes the oxidation of the colorless substrate, yielding a blue product. The reaction is then stopped by adding a stop solution (usually an acid), which changes the color from blue to yellow. The optical density (OD) of each well is measured at 450 nm using a microplate reader. The concentration ofMouse ACE is directly proportional to the OD450 value. A standard curve is generated by assaying known concentrations ofMouse ACE, and the concentration of Mouse ACE in unknown samples is interpolated from this curve based on their OD values.
Storage and Shipping
| Shipped In | Wet ice |
| Stability and Storage | Store at 2-8℃ for 6 months. |
| Storage Temp | Store at 2-8°C |
Additional Information
| Detected Species Object | Mouse |
| Detection Range | 93.8 pg/mL~6,000 pg/mL |
| Method OF Detection | Sandwich ELISA |
| Specificity | Detecting Mouse ACE in samples, with no significant cross-reactivity to other homologs. |
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