Rat IL-3 ELISA Kit R1509908-96T Aladdin
$599.00
In stock
SKU
R1509908-96T
Product Information
| Product Name | Rat IL-3 ELISA Kit |
| Specifications & Purity | BioReagent |
Product Description
This kit employs a sandwich enzyme-linked immunosorbent assay (ELISA) for the quantitative detection of Rat IL-3 concentration in samples. The Rat IL-3 capture antibody is pre-coated onto the microtiter plate. Upon addition of samples or standards, Rat IL-3 present therein binds to the immobilized capture antibody, while other unbound components are removed through washing. Subsequently, a biotin-conjugated detection antibody specific for Rat IL-3 is added. This antibody binds to the captured Rat IL-3, forming a "sandwich" immunocomplex. Any excess, unbound material is again removed by washing. Next, an enzyme conjugate (typically Streptavidin-Horseradish Peroxidase, SA-HRP) is added. The biotin on the detection antibody and streptavidin on the enzyme conjugate exhibit high-affinity binding, thereby linking the HRP enzyme to the immunocomplex.
Following another wash step to remove unbound conjugate, a colorimetric substrate (e.g., TMB) is added. If Rat IL-3 is present in the sample, the HRP linked to the complex catalyzes the oxidation of the colorless substrate, yielding a blue product. The reaction is then stopped by adding a stop solution (usually an acid), which changes the color from blue to yellow. The optical density (OD) of each well is measured at 450 nm using a microplate reader. The concentration ofRat IL-3 is directly proportional to the OD450 value. A standard curve is generated by assaying known concentrations ofRat IL-3, and the concentration of Rat IL-3 in unknown samples is interpolated from this curve based on their OD values.
Storage and Shipping
| Shipped In | Wet ice |
| Stability and Storage | Store at 2-8℃ for 6 months. |
| Storage Temp | Store at 2-8°C |
Additional Information
| Detected Species Object | Rat |
| Detection Range | 7.8 pg/mL~500 pg/mL |
| Method OF Detection | Sandwich ELISA |
| Specificity | Detecting Rat IL-3 in samples, with no significant cross-reactivity to other homologs. |
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