Human Cytokeratin 19/KRT19/CYFRA21-1 ELISA Kit H1509991-96T Aladdin
$599.00
In stock
SKU
H1509991-96T
Product Information
| Product Name | Human Cytokeratin 19/KRT19/CYFRA21-1 ELISA Kit |
| Specifications & Purity | BioReagent |
Product Description
This kit employs a sandwich enzyme-linked immunosorbent assay (ELISA) for the quantitative detection of Human Cytokeratin 19/KRT19/CYFRA21-1 concentration in samples. The Human Cytokeratin 19/KRT19/CYFRA21-1 capture antibody is pre-coated onto the microtiter plate. Upon addition of samples or standards, Human Cytokeratin 19/KRT19/CYFRA21-1 present therein binds to the immobilized capture antibody, while other unbound components are removed through washing. Subsequently, a biotin-conjugated detection antibody specific for Human Cytokeratin 19/KRT19/CYFRA21-1 is added. This antibody binds to the captured Human Cytokeratin 19/KRT19/CYFRA21-1, forming a "sandwich" immunocomplex. Any excess, unbound material is again removed by washing. Next, an enzyme conjugate (typically Streptavidin-Horseradish Peroxidase, SA-HRP) is added.
The biotin on the detection antibody and streptavidin on the enzyme conjugate exhibit high-affinity binding, thereby linking the HRP enzyme to the immunocomplex. Following another wash step to remove unbound conjugate, a colorimetric substrate (e.g., TMB) is added. If Human Cytokeratin 19/KRT19/CYFRA21-1 is present in the sample, the HRP linked to the complex catalyzes the oxidation of the colorless substrate, yielding a blue product. The reaction is then stopped by adding a stop solution (usually an acid), which changes the color from blue to yellow. The optical density (OD) of each well is measured at 450 nm using a microplate reader. The concentration ofHuman Cytokeratin 19/KRT19/CYFRA21-1 is directly proportional to the OD450 value.
A standard curve is generated by assaying known concentrations ofHuman Cytokeratin 19/KRT19/CYFRA21-1, and the concentration of Human Cytokeratin 19/KRT19/CYFRA21-1 in unknown samples is interpolated from this curve based on their OD values.
Storage and Shipping
| Shipped In | Wet ice |
| Stability and Storage | Store at 2-8℃ for 6 months. |
| Storage Temp | Store at 2-8°C |
Additional Information
| Detected Species Object | Human |
| Detection Range | 0.312 ng/mL~20 ng/mL |
| Method OF Detection | Sandwich ELISA |
| Specificity | Detecting Human Cytokeratin 19/KRT19/CYFRA21-1 in samples, with no significant cross-reactivity to other homologs. |
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