PlusPlasma Low Abundance Protein Enrichment and Pretreatment Kit P1456360-10T Aladdin
$559.00
In stock
SKU
P1456360-10T
Product Information
| Product Name | PlusPlasma Low Abundance Protein Enrichment and Pretreatment Kit |
| Specifications & Purity | BioReagent, sterile, Proteomics grade |
| Synonyms | Low Abundance Protein Enrichment and Pretreatment Kit |
Product Description
Product Introduction:
This product is a one-stop processing kit from plasma to peptides. It utilizes nanotechnology to effectively enrich low-abundance proteins in plasma and processes plasma proteins in combination with the innovative concept of "All In One Tube". Even researchers without omics background can quickly prepare pretreated samples, which can be directly used for subsequent mass spectrometry analysis.
Product Components and Storage Conditions:
P1456360
Component
10T
25T
Storage
P1456360A
Magnetic beads
10T
25T
4℃
P1456360B
Incubation Buffer I
4.8 mL
12 mL
RT
P1456360C
Incubation Buffer II
6 mL
15 mL
RT
P1456360D
Washing Buffer
6 mL
15 mL
RT
P1456360E
Lysis Buffer
0.6 mL
1.5 mL
-20℃. Store in the dark.
P1456360F
Balance Buffer
2.8mL
7 mL
4℃
P1456360G
Digest Buffer
16 μL
40 μL
-20℃
P1456360H
Stop Buffer
300 μL
750 μL
RT
P1456360I
Wash Buffer I
4 mL
10 mL
RT
P1456360J
Wash Buffer II
2.4 mL
6 mL
RT
P1456360K
Wash Buffer III
2.4 mL
6 mL
RT
P1456360L
Elution Buffer
4.8 mL
12 mL
RT. Store in the dark.
P1456360M
Loading Buffer
120 μL
300 μL
RT
P1456360N
Tip柱
10T
25T
RT
Product Features:
Convenient and fast: The processing from plasma to peptides can be completed with one kit.
Simple operation: The instruments used are simple, requiring only a conventional centrifuge and a metal bath.
Good stability: Each batch undergoes strict quality inspection, ensuring high reproducibility of experimental results.
Operating Procedure:
1.Centrifuge the plasma sample (3000g, 10min), and take the supernatant for later use (if storage is required, store it at -80°C for long-term preservation to avoid repeated freezing and thawing).
2.Take 50-100μL of centrifuged plasma, add 400μL of Incubation buffer I, then add 30μL of Magnetic beads, vortex to mix, and incubate at room temperature on a shaking mixer for 1 hour.
3.After incubation, use a magnetic rack to magnetically separate for 3min, and discard the supernatant.
4.Remove the EP tube, add 500μL of Incubation buffer II, gently invert up and down to mix several times, magnetically separate for 3min, and discard the supernatant.
5.Remove the EP tube, add 500μL of Washing Buffer, gently invert up and down to mix several times, magnetically separate for 3min, and discard the supernatant.
6.Remove the EP tube, add 50μL of Lysis Buffer to resuspend the beads, place in a water bath at 95°C for 10min, then take it out and cool to room temperature.
7.Add 225μL of Balance buffer to the EP tube that has cooled to room temperature.
8.Add 1μL of Digest buffer to the sample, and perform enzymatic digestion with shaking in a metal bath at 37°C and 1200rpm for 3-16h.
9.After enzymat
ic digestion, take out the EP tube, add 25μL of Stop buffer to the sample, and vortex to mix.
10.Add 320μL of
Wash buffer I, shake vigorously for 3min, centrifuge at 15000rpm for 3min, and remove the upper liquid.
11.Transfer the lower layer sample into the Tip column, centrifuge at 2500rpm for 3-5min until all the liquid is centrifuged down. If the liquid flow rate is slow, the rotation speed can be appropriately increased.
12.Add 200μL of
Wash buffer II
(shake for 10-20s before use) to the desalting column, centrifuge at 2500rpm for 3-5min until all the liquid is centrifuged down.
13.Add 200μL of
Wash buffer III
to the
desalting column, centrifuge at 2500rpm for 3-5min until all the liquid is centrifuged down.
14.Put the desalting column into a new EP tube, add 200μL of Elution buffer to the desalting column, centrifuge at 2000rpm for 3-5min until all the liquid is centrifuged down.
15.Repeat the previous step, collect the eluates from both times, and freeze-dry them.
16.Add 10μL of Loading buffer, vortex vigorously for 3min, centrifuge at 2000g for 10min, take an appropriate amount of sample for mass spectrometry detection. Taking the HF-X instrument as an example, 0.5-1μg of sample is sufficient for loading.
Automated Operation Process:
It is compatible with mass spectrometry proteomics pretreatment workstations, enabling one-stop processing from plasma to peptides. This eliminates manual operation errors, improves the reproducibility of sample preparation workflows, and provides high precision and reliability for various laboratory procedures.
Storage and Shipping
| Shipped In | Ice chest + Ice pads |
| Stability and Storage | P1456360A、P1456360F: Store at 2-8℃ long term (12 months). P1456360E: Store at -20℃ long term (12 months). Store in the dark. P1456360G: Store at -20℃ long term (12 months). P1456360L: Store at room temperature long term (12 months). Store in the dark. P14 |
| Storage Temp | Store at 2-8°C,Protected from light,Room temperature,Store at -20°C |
Additional Information
| Sensitivity | Light-sensitive |
MDSD
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