Citations：260
| CAS：77-86-1 | Molecular Formula：C4H11NO3 |
| Molecular Weight：121.14 | Storage：Store at RT，7 years |
| Appearance：White to light yellow crystalline powder | Purity：≥99.5% |
| Name Tris (Hydroxymethyl) Aminomethane | CAS 77-86-1 |
| Molecular Formula C4H11NO3 | Molecular Weight 121.14 |
| Storage Store at RT，7 years | Specification 5kg ; 100g ; 1kg ; 500g |
| Appearance White to light yellow crystalline powder | Purity ≥99.5% |
| Grade Ultra Pure Grade | Solubility 121mg/mL in Water |
| Unit Bottle | Tris is an organic compound, white to slightly yellow crystalline powder or block, chemical formula C4H11NO3, soluble in ethanol and water, slightly soluble in ethyl acetate, benzene, insoluble in ether, carbon tetrachloride. Solubility at 25℃ (mg/mL) : water 550, ethylene glycol 79.1, methanol 26, absolute ethanol 14.6, 95% ethanol 22.0, N, N-dimethylformamide 14, pentanol 2.6, propanol 2.0, ethyl acetate 0.5, cyclohexane 0.1. The aqueous solution does not absorb carbon dioxide and is corrosive and irritating to copper and aluminum. It is a solvent for nucleic acids and proteins and is widely used in the preparation of buffers in biochemical and molecular biology experiments. |
| Tris is a weak base with a pKa of 8.1 at 25 ℃. According to buffer theory, the effective buffer range of Tris buffer is between pH7.0 and 9.2. The pH of 0.1mol/l aqueous solution is 10.4, and generally hydrochloric acid is added to adjust the pH value to obtain the buffer with the desired pH value. But at the same time, attention should be paid to the effect of temperature on the pKa of Tris. For Tris buffer, for every 1 ℃ decrease in temperature, the pH increases by about 0.03 units, and for every 10 times dilution of concentration, the pH decreases by 0.03-0.05 units. For precise applications, a carefully calibrated pH meter with a glass/calomel composite electrode is used. | 1. 1M Tris-HCl 6.8, 1.5M Tris-HCl 8.8 and 5× Tris-glycine electrophoresis buffer were the most commonly used reagents for SDS-PAGE. |
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