Sodium Citrate buffer(0.01mol/L, pH6.0, powder)
$16.00
In stock
SKU
C1010
Citations：198
| Storage：Store at RT，1 year. | Appearance：White powder |
| Purity：0.01M | |
| Name Sodium Citrate buffer(0.01mol/L, pH6.0, powder) | Storage Store at RT，1 year. |
| Specification 10*2L ; 100*1L ; 100*2L ; 10*1L | Appearance White powder |
| Purity 0.01M | Unit Bag |
| After the cell or tissue is fixed with paraformaldehyde, formaldehyde or other aldehydes, the intracellular antigen will form aldehyde bonds and carboxymethyl bonds, and part of the antigenic determinant will be blocked. At the same time, the cross-linking between proteins will make the antigenic determinant hidden, resulting in weakened staining signal during immune staining, and even some false negative staining results. Therefore, when immunohistochemical staining is performed, antigen repair or exposure is required first, that is, the cross-linking formed between molecules during fixation is destroyed, and the original spatial form of the antigen is restored. So the detection rate of antigen can be improved, the background staining can be reduced, and the accuracy of detection can be improved. | |
| Usually paraffin sections need to be treated with antigen repair, while frozen sections can not be treated with antigen repair. Antigen repair can improve the immunostaining effect of paraffin section and the staining effect of frozen section. When the immunostaining effect of frozen section is not satisfactory, antigen repair should be considered. | Note：Product information may be optimized and upgraded. Please refer to the actual label information for accuracy. |
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