Human G1/S-specific cyclin-E1(CCNE1) ELISA kit CSB-EL004817HU CUSABIO
$50.00
In stock
SKU
CSB-EL004817HU
Product Details
| Target Name | cyclin E1 |
| Uniprot No. | P24864 |
| Species | Homo sapiens (Human) |
| Storage | Store at 2-8°C. Please refer to protocol. |
| Sensitivity | 6.25 pg/mL |
| Troubleshooting and FAQs | ELISA kit FAQs |
| Lead Time | 3-5 working days after you place the order, and it takes another 3-5 days for delivery via DHL or FedEx. |
| Associated names | CCNE ELISA Kit; Ccne1 ELISA Kit; CCNE1_HUMAN ELISA Kit; cyclin E variant ex5del ELISA Kit; cyclin E variant ex7del ELISA Kit; Cyclin E1 ELISA Kit; Cyclin Es ELISA Kit; Cyclin Et ELISA Kit; CyclinE ELISA Kit; G1/S specific cyclin E ELISA Kit; G1/S-specific cyclin-E1 ELISA Kit |
| Abbreviation | CCNE1 |
| Sample Types | serum, plasma, tissue homogenates, cell lysates |
| Detection Range | 25 pg/mL-1600 pg/mL |
| Assay Time | 1-5h |
| Sample Loading Volume | 50-100ul |
| Detection Wavelength | 450 nm |
| Research Area | Cell Biology |
| Assay Principle | quantitative |
| Measurement | Sandwich |
| Precision | headers: Intra-assay Precision (Precision within an assay): CV%<8%; rows: Intra-assay Precision (Precision within an assay): CV%<8%: Intra-assay Precision (Precision within an assay): CV%<8%; 1: Three samples of known concentration were tested twenty times on one plate to assess.; Intra-assay Precision (Precision within an assay): CV%<8%: Inter-assay Precision (Precision between assays): CV%<10%; 1: Three samples of known concentration were tested in twenty assays to assess.; |
| Linearity | headers: To assess the linearity of the assay, samples were spiked with high concentrations of human CCNE1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.; rows: To assess the linearity of the assay, samples were spiked with high concentrations of human CCNE1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.: To assess the linearity of the assay, samples were spiked with high concentrations of human CCNE1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.; 2: Sample; 3: Serum(n=4); 1: 1:1; 2: Average %; 3: 100; 1: Range %; 2: 95-104; 1: 1:2; 2: Average %; 3: 95; 1: Range %; 2: 90-100; 1: 1:4; 2: Average %; 3: 91; 1: Range %; 2: 86-95; 1: 1:8; 2: Average %; 3: 84; 1: Range %; 2: 80-88 |
| Recovery | headers: The recovery of human CCNE1 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.; rows: The recovery of human CCNE1 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.: The recovery of human CCNE1 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.; ; 1: Sample Type; 2: Average % Recovery; 3: Range; 1: Serum (n=5); 2: 93; 3: 89-97; 1: EDTA plasma (n=4); 2: 88; 3: 84-92; ; |
| Typical Data | headers: These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.; rows: These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.: These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.; ; 1: pg/ml OD1 OD2 Average Corrected 1600 1.921 1.837 1.879 1.741 800 1.294 1.228 1.261 1.123 400 0.819 0.783 0.801 0.663 200 0.543 0.519 0.531 0.393 100 0.362 0.376 0.369 0.231 50 0.280 0.301 0.291 0.153 25 0.207 0.194 0.201 0.063 0 0.141 0.135 0.138; 2: pg/ml; 3: OD1; 4: OD2; 5: Average; 6: Corrected; 9: 1600; 10: 1.921; 11: 1.837; 12: 1.879; 13: 1.741; 16: 800; 17: 1.294; 18: 1.228; 19: 1.261; 20: 1.123; 23: 400; 24: 0.819; 25: 0.783; 26: 0.801; 27: 0.663; 30: 200; 31: 0.543; 32: 0.519; 33: 0.531; 34: 0.393; 37: 100; 38: 0.362; 39: 0.376; 40: 0.369; 41: 0.231; 44: 50; 45: 0.280; 46: 0.301; 47: 0.291; 48: 0.153; 51: 25; 52: 0.207; 53: 0.194; 54: 0.201; 55: 0.063; 58: 0; 59: 0.141; 60: 0.135; 61: 0.138; 1: pg/ml; 2: OD1; 3: OD2; 4: Average; 5: Corrected; 1: 1600; 2: 1.921; 3: 1.837; 4: 1.879; 5: 1.741; 1: 800; 2: 1.294; 3: 1.228; 4: 1.261; 5: 1.123; 1: 400; 2: 0.819; 3: 0.783; 4: 0.801; 5: 0.663; 1: 200; 2: 0.543; 3: 0.519; 4: 0.531; 5: 0.393; 1: 100; 2: 0.362; 3: 0.376; 4: 0.369; 5: 0.231; 1: 50; 2: 0.280; 3: 0.301; 4: 0.291; 5: 0.153; 1: 25; 2: 0.207; 3: 0.194; 4: 0.201; 5: 0.063; 1: 0; 2: 0.141; 3: 0.135; 4: 0.138 |
| ELISA Data Analysis | ELISA Standard Curve & Curve Expert software |
| Shelf Life | 6 months |
| Description | This Human CCNE1 ELISA Kit was designed for the quantitative measurement of Human CCNE1 protein in serum, plasma, tissue homogenates, cell lysates. It is a Sandwich ELISA kit, its detection range is 25 pg/mL-1600 pg/mL and the sensitivity is 6.25 pg/mL. |
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