Human Hyaluronan-binding protein 2(HABP2) ELISA kit CSB-EL010113HU CUSABIO
$50.00
In stock
SKU
CSB-EL010113HU
Product Details
| Target Name | hyaluronan binding protein 2 |
| Uniprot No. | Q14520 |
| Species | Homo sapiens (Human) |
| Storage | Store at 2-8°C. Please refer to protocol. |
| Sensitivity | 0.078 ng/mL |
| Troubleshooting and FAQs | ELISA kit FAQs |
| Lead Time | 3-5 working days after you place the order, and it takes another 3-5 days for delivery via DHL or FedEx. |
| Associated names | Factor seven activating protease ELISA Kit; Factor seven-activating protease ELISA Kit; Factor VII activating protein ELISA Kit; Factor VII-activating protease ELISA Kit; FSAP ELISA Kit; HABP 2 ELISA Kit; HABP ELISA Kit; Habp2 ELISA Kit; HABP2_HUMAN ELISA Kit; Hepatocyte growth factor activator like protein ELISA Kit; Hepatocyte growth factor activator-like protein ELISA Kit; HGFAL ELISA Kit; Hyaluronan binding protein 2 ELISA Kit; Hyaluronan-binding protein 2 27 kDa light chain alternate form ELISA Kit; Hyaluronic acid binding protein 2 ELISA Kit; PHBP ELISA Kit; Plasma hyaluronan binding protein ELISA Kit; Plasma hyaluronan-binding protein ELISA Kit |
| Abbreviation | HABP2 |
| Sample Types | serum, plasma, tissue homogenates |
| Detection Range | 0.312 ng/mL-20 ng/mL |
| Assay Time | 1-5h |
| Sample Loading Volume | 50-100ul |
| Detection Wavelength | 450 nm |
| Research Area | Cardiovascular |
| Assay Principle | quantitative |
| Measurement | Sandwich |
| Precision | headers: Intra-assay Precision (Precision within an assay): CV%<8%; rows: Intra-assay Precision (Precision within an assay): CV%<8%: Intra-assay Precision (Precision within an assay): CV%<8%; 1: Three samples of known concentration were tested twenty times on one plate to assess.; Intra-assay Precision (Precision within an assay): CV%<8%: Inter-assay Precision (Precision between assays): CV%<10%; 1: Three samples of known concentration were tested in twenty assays to assess.; |
| Linearity | headers: To assess the linearity of the assay, samples were spiked with high concentrations of human HABP2 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.; rows: To assess the linearity of the assay, samples were spiked with high concentrations of human HABP2 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.: To assess the linearity of the assay, samples were spiked with high concentrations of human HABP2 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.; 2: Sample; 3: Serum(n=4); 1: 1:20; 2: Average %; 3: 88; 1: Range %; 2: 82-96; 1: 1:40; 2: Average %; 3: 89; 1: Range %; 2: 85-95; 1: 1:80; 2: Average %; 3: 96; 1: Range %; 2: 91-101; 1: 1:160; 2: Average %; 3: 100; 1: Range %; 2: 97-103 |
| Recovery | headers: The recovery of human HABP2 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.; rows: The recovery of human HABP2 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.: The recovery of human HABP2 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.; 1: Sample Type; 2: Average % Recovery; 3: Range; 1: Serum (n=5); 2: 103; 3: 99-105; 1: EDTA plasma (n=4); 2: 92; 3: 85-98; ; |
| Typical Data | headers: These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.; rows: These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.: These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.; 1: ng/ml OD1 OD2 Average Corrected 20 2.875 2.775 2.825 2.725 10 2.524 2.345 2.435 2.335 5 1.818 1.787 1.803 1.703 2.5 0.964 0.901 0.933 0.833 1.25 0.602 0.586 0.594 0.494 0.625 0.400 0.425 0.413 0.313 0.312 0.205 0.195 0.200 0.100 0 0.101 0.098 0.100; 2: ng/ml; 3: OD1; 4: OD2; 5: Average; 6: Corrected; 8: 20; 9: 2.875; 10: 2.775; 11: 2.825; 12: 2.725; 14: 10; 15: 2.524; 16: 2.345; 17: 2.435; 18: 2.335; 20: 5; 21: 1.818; 22: 1.787; 23: 1.803; 24: 1.703; 26: 2.5; 27: 0.964; 28: 0.901; 29: 0.933; 30: 0.833; 32: 1.25; 33: 0.602; 34: 0.586; 35: 0.594; 36: 0.494; 38: 0.625; 39: 0.400; 40: 0.425; 41: 0.413; 42: 0.313; 44: 0.312; 45: 0.205; 46: 0.195; 47: 0.200; 48: 0.100; 50: 0; 51: 0.101; 52: 0.098; 53: 0.100; 1: ng/ml; 2: OD1; 3: OD2; 4: Average; 5: Corrected; 1: 20; 2: 2.875; 3: 2.775; 4: 2.825; 5: 2.725; 1: 10; 2: 2.524; 3: 2.345; 4: 2.435; 5: 2.335; 1: 5; 2: 1.818; 3: 1.787; 4: 1.803; 5: 1.703; 1: 2.5; 2: 0.964; 3: 0.901; 4: 0.933; 5: 0.833; 1: 1.25; 2: 0.602; 3: 0.586; 4: 0.594; 5: 0.494; 1: 0.625; 2: 0.400; 3: 0.425; 4: 0.413; 5: 0.313; 1: 0.312; 2: 0.205; 3: 0.195; 4: 0.200; 5: 0.100; 1: 0; 2: 0.101; 3: 0.098; 4: 0.100 |
| ELISA Data Analysis | ELISA Standard Curve & Curve Expert software |
| Shelf Life | 6 months |
| Description | The human HABP2 ELISA Kit is engineered for accurate measurement of human HABP2 levels from samples including serum, plasma, or tissue homogenates. It uses the Sandwich-ELISA mechanism in combination with the enzyme-substrate chromogenic reaction to measure the human HABP2 content in the sample. The color intensity is positively correlated with HABP2 content in the sample. This kit has been validated against standards of sensitivity, specificity, precision, linearity, recovery, and lot-to-lot consistency. HABP2, also called FSAP, is an extracellular serine protease involved in the extrinsic pathway of blood coagulation via activation of factor VII and fibrinolysis via activation of pro-urokinase type plasminogen activator (pro-uPA). It negatively regulates vascular integrity via activation of PAR receptor/RhoA/ROCK signaling. It has been implicated in several disease processes including atherosclerosis and deep venous thrombosis. Upregulation of HABP2 has been found in the pulmonary vasculature with acute lung injury (ALI) and promotes disruption of vascular integrity. |
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