Human Hyaluronidase PH-20(SPAM1) ELISA kit CSB-EL022471HU-IS CUSABIO
$50.00
In stock
SKU
CSB-EL022471HU-IS
Product Details
| Target Name | sperm adhesion molecule 1 (PH-20 hyaluronidase, zona pellucida binding) |
| Uniprot No. | P38567 |
| Species | Homo sapiens (Human) |
| Storage | Store at 2-8°C. Please refer to protocol. |
| Sensitivity | 39 pg/mL |
| Troubleshooting and FAQs | ELISA kit FAQs |
| Lead Time | 3-5 working days after you place the order, and it takes another 3-5 days for delivery via DHL or FedEx. |
| Associated names | epididymis secretory sperm binding protein Li 96n ELISA Kit; HEL-S-96n ELISA Kit; HYA1 ELISA Kit; HYAL PH-20 ELISA Kit; HYAL PH20 ELISA Kit; Hyal-PH20 ELISA Kit; HYAL1 ELISA Kit; HYAL3 ELISA Kit; HYAL5 ELISA Kit; HYALP_HUMAN ELISA Kit; Hyaluronidase PH-20 ELISA Kit; Hyaluronoglucosaminidase PH-20 ELISA Kit; MGC26532 ELISA Kit; PH-20 Hyaluronidase ELISA Kit; PH20 ELISA Kit; PH20 Hyaluronidase ELISA Kit; SPAG15 ELISA Kit; SPAM-1 ELISA Kit; Spam1 ELISA Kit; sperm adhesion molecule 1 (PH-20 hyaluronidase, zona pellucida binding) ELISA Kit; Sperm adhesion molecule 1 ELISA Kit; Sperm surface protein PH-20 ELISA Kit; Sperm surface protein PH20 ELISA Kit; zona pellucida binding ELISA Kit |
| Abbreviation | SPAM1 |
| Sample Types | cell culture supernates |
| Detection Range | 78 pg/mL-5000 pg/mL |
| Assay Time | 1-5h |
| Sample Loading Volume | 50-100ul |
| Detection Wavelength | 450 nm |
| Research Area | Signal Transduction |
| Assay Principle | quantitative |
| Measurement | Sandwich |
| Precision | headers: Intra-assay Precision (Precision within an assay): CV%<8%; rows: Intra-assay Precision (Precision within an assay): CV%<8%: Intra-assay Precision (Precision within an assay): CV%<8%; 1: Three samples of known concentration were tested twenty times on one plate to assess.; Intra-assay Precision (Precision within an assay): CV%<8%: Inter-assay Precision (Precision between assays): CV%<10%; 1: Three samples of known concentration were tested in twenty assays to assess. |
| Linearity | headers: To assess the linearity of the assay, samples were spiked with high concentrations of human SPAM1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.; rows: To assess the linearity of the assay, samples were spiked with high concentrations of human SPAM1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.: To assess the linearity of the assay, samples were spiked with high concentrations of human SPAM1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.; 2: Sample; 3: Cell Culture Supernates(n=4); 1: 1:400; 2: Average %; 3: 87; 1: Range %; 2: 81-95; 1: 1:800; 2: Average %; 3: 93; 1: Range %; 2: 89-100; 1: 1:1600; 2: Average %; 3: 106; 1: Range %; 2: 99-110; 1: 1:3200; 2: Average %; 3: 90; 1: Range %; 2: 82-93 |
| Recovery | headers: The recovery of human SPAM1 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.; rows: The recovery of human SPAM1 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.: The recovery of human SPAM1 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.; 1: Sample Type; 2: Average % Recovery; 3: Range; 1: l Cell Culture Supernates (n=5); 2: 85; 3: 80-92 |
| Typical Data | headers: These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.; rows: These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.: These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.; ; 1: pg/ml; 2: OD1; 3: OD2; 4: Average; 5: Corrected; 1: 5000; 2: 3.654; 3: 3.670; 4: 3.662; 5: 3.576; 1: 2500; 2: 3.017; 3: 3.123; 4: 3.070; 5: 2.984; 1: 1250; 2: 2.138; 3: 2.192; 4: 2.165; 5: 2.079; 1: 625; 2: 1.137; 3: 1.194; 4: 1.166; 5: 1.080; 1: 312; 2: 0.684; 3: 0.708; 4: 0.696; 5: 0.610; 1: 156; 2: 0.418; 3: 0.439; 4: 0.428; 5: 0.343; 1: 78; 2: 0.226; 3: 0.220; 4: 0.223; 5: 0.137; 1: 0; 2: 0.085; 3: 0.087; 4: 0.086 |
| ELISA Data Analysis | ELISA Standard Curve & Curve Expert software |
| Shelf Life | 6 months |
| Description | This ELISA kit provides quantitative measurement of human SPAM1 (sperm adhesion molecule 1/hyaluronidase PH-20) in research contexts spanning signal transduction and reproductive biology. Because SPAM1 is a GPI-anchored hyaluronidase that can be released from cell surfaces into conditioned media, detecting its soluble form at low concentrations demands purpose-built assay sensitivity. Detection Range: The 78–5000 pg/mL range captures the low-to-moderate concentrations of shed SPAM1 typically found in cell culture supernates from transfected lines or primary reproductive tract cells, minimizing the need for extensive sample concentration while accommodating higher-expression systems through simple dilution. Sensitivity: At 39 pg/mL, the kit detects SPAM1 in conditioned media from cells with modest endogenous expression, including epididymal or cumulus cell cultures where soluble PH-20 levels may be near the lower physiological threshold. Sample Compatibility: Validation for cell culture supernates directly supports in vitro studies of SPAM1 shedding mechanisms, GPI-anchor cleavage regulation, and hyaluronidase activity modulation without requiring cell lysis or membrane fractionation. Assay Efficiency: The 1–5 hour protocol and 50–100 µL sample volume accommodate experiments where conditioned media volumes are limited, such as small-scale primary spermatocyte or organoid cultures, enabling same-day quantification across multiple treatment conditions. This kit is well suited for studies of sperm-oocyte interaction, cumulus matrix remodeling, and SPAM1-mediated signaling in fertilization research. |
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