Human Interferon β,IFN-β/IFNB ELISA Kit CSB-E09889h-IS CUSABIO
$50.00
In stock
SKU
CSB-E09889h-IS
Product Details
| Target Name | interferon, beta 1,fibroblast |
| Uniprot No. | P01574 |
| Species | Homo sapiens (Human) |
| Storage | Store at 2-8°C. Please refer to protocol. |
| Sensitivity | 15.6pg/mL |
| Troubleshooting and FAQs | ELISA kit FAQs |
| Lead Time | 3-5 working days |
| Associated names | beta-interferon ELISA Kit; Fibroblast interferon ELISA Kit; IFB ELISA Kit; IFF ELISA Kit; IFN beta ELISA Kit; IFN-beta ELISA Kit; IFNB 1 ELISA Kit; IFNB ELISA Kit; IFNB_HUMAN ELISA Kit; IFNB1 ELISA Kit; Interferon beta 1 fibroblast ELISA Kit; Interferon beta ELISA Kit; Interferon beta precursor ELISA Kit; MGC96956 ELISA Kit |
| Abbreviation | IFNB1 |
| Sample Types | serum, plasma, cell culture supernates, saliva, tissue homogenates |
| Detection Range | 31.25pg/mL-2000pg/mL |
| Assay Time | 1-5h |
| Sample Loading Volume | 50-100ul |
| Detection Wavelength | 450 nm |
| Research Area | Immunology |
| Assay Principle | quantitative |
| Measurement | Sandwich |
| Precision | headers: Intra-assay Precision (Precision within an assay): CV%<8%; rows: Intra-assay Precision (Precision within an assay): CV%<8%: Intra-assay Precision (Precision within an assay): CV%<8%; 1: Three samples of known concentration were tested twenty times on one plate to assess.; Intra-assay Precision (Precision within an assay): CV%<8%: Inter-assay Precision (Precision between assays): CV%<10%; 1: Three samples of known concentration were tested in twenty assays to assess. |
| Linearity | headers: To assess the linearity of the assay, samples were spiked with high concentrations of human IFN-β/IFNB in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.; rows: To assess the linearity of the assay, samples were spiked with high concentrations of human IFN-β/IFNB in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.: To assess the linearity of the assay, samples were spiked with high concentrations of human IFN-β/IFNB in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.; 2: Sample; 3: Serum(n=4); 1: 1:1; 2: Average %; 3: 98; 1: Range %; 2: 91-105; 1: 1:2; 2: Average %; 3: 92; 1: Range %; 2: 88-97; 1: 1:4; 2: Average %; 3: 97; 1: Range %; 2: 91-105; 1: 1:8; 2: Average %; 3: 93; 1: Range %; 2: 86-99 |
| Recovery | headers: The recovery of human IFN-β/IFNB spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.; rows: The recovery of human IFN-β/IFNB spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.: The recovery of human IFN-β/IFNB spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.; 1: Sample Type; 2: Average % Recovery; 3: Range; 1: Serum (n=5); 2: 91; 3: 84-98; 1: EDTA plasma (n=4); 2: 97; 3: 90-104 |
| Typical Data | headers: These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.; rows: These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.: These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.; ; 1: pg/ml; 2: OD1; 3: OD2; 4: Average; 5: Corrected; 1: 2000; 2: 3.098; 3: 2.934; 4: 3.016; 5: 2.899; 1: 1000; 2: 2.131; 3: 2.267; 4: 2.199; 5: 2.082; 1: 500; 2: 1.320; 3: 1.348; 4: 1.334; 5: 1.217; 1: 250; 2: 0.666; 3: 0.695; 4: 0.681; 5: 0.563; 1: 125; 2: 0.382; 3: 0.399; 4: 0.390; 5: 0.273; 1: 62.5; 2: 0.257; 3: 0.268; 4: 0.262; 5: 0.145; 1: 31.25; 2: 0.190; 3: 0.184; 4: 0.187; 5: 0.070; 1: 0; 2: 0.120; 3: 0.115; 4: 0.117 |
| ELISA Data Analysis | ELISA Standard Curve & Curve Expert software |
| Shelf Life | 6 months |
| Description | This ELISA kit provides quantitative measurement of human interferon-beta (IFNB1) in biological samples, supporting research into type I interferon-mediated innate and adaptive immune responses. Because IFNB1 is rapidly secreted at low concentrations following viral sensing or autoimmune flare and varies dramatically across sample matrices, selecting an assay calibrated to its expected biological range is critical. Detection Range: The 31.25–2000 pg/mL range spans from baseline circulating IFNB1 levels through the elevated concentrations observed during acute viral infection or in stimulated PBMC supernatants, minimizing the need for extensive sample dilution or concentration steps in most experimental contexts. Sensitivity: At 15.6 pg/mL, this kit detects IFNB1 in unstimulated or early-timepoint samples where interferon production is just initiating — critical for capturing the kinetics of innate antiviral signaling before peak secretion occurs. Sample Compatibility: Validation across serum, plasma, cell culture supernatants, saliva, and tissue homogenates enables researchers to correlate systemic IFNB1 levels with local tissue production or in vitro stimulation data within a single study, bridging clinical and mechanistic experiments. Assay Efficiency: Requiring only 50–100 µL and 1–5 hours, the protocol accommodates limited-volume samples from primary plasmacytoid dendritic cell cultures or longitudinal patient collections without exhausting precious biobank material. This kit is well-suited for studies in multiple sclerosis therapeutic monitoring, COVID-19 innate immune profiling, and interferonopathy characterization. |
Reviews
Write Your Own Review
Related Products
Check items to add to the cart or
