Human Interleukin 1β,IL-1β ELISA Kit CSB-E08053h-IS CUSABIO
$50.00
In stock
SKU
CSB-E08053h-IS
Product Details
| Uniprot No. | P01584 |
| Species | Homo sapiens (Human) |
| Storage | Store at 2-8°C. Please refer to protocol. |
| Sensitivity | 0.586 pg/ml |
| Troubleshooting and FAQs | ELISA kit FAQs |
| Lead Time | 3-5 working days after you place the order, and it takes another 3-5 days for delivery via DHL or FedEx. |
| Associated names | Catabolin ELISA Kit; H1 ELISA Kit; IFN beta inducing factor ELISA Kit; IL 1 ELISA Kit; IL 1 beta ELISA Kit; IL-1 beta ELISA Kit; IL1 ELISA Kit; IL1 BETA ELISA Kit; IL1B ELISA Kit; IL1B_HUMAN ELISA Kit; IL1F2 ELISA Kit; Interleukin 1 beta ELISA Kit; Interleukin 1 beta precursor ELISA Kit; interleukin 1; beta ELISA Kit; Interleukin-1 beta ELISA Kit; OAF ELISA Kit; Osteoclast activating factor ELISA Kit; OTTHUMP00000162031 ELISA Kit; Preinterleukin 1 beta ELISA Kit; Preinterleukin beta ELISA Kit; Pro interleukin 1 beta ELISA Kit |
| Abbreviation | IL1B |
| Sample Types | serum, plasma, tissue homogenates, cell culture supernates |
| Detection Range | 2.344 pg/ml-150 pg/ml |
| Assay Time | 1-5h |
| Sample Loading Volume | 50-100ul |
| Detection Wavelength | 450 nm |
| Research Area | Immunology |
| Assay Principle | quantitative |
| Measurement | Sandwich |
| Precision | headers: Intra-assay Precision (Precision within an assay): CV%<8%; rows: Intra-assay Precision (Precision within an assay): CV%<8%: Intra-assay Precision (Precision within an assay): CV%<8%; 1: Three samples of known concentration were tested twenty times on one plate to assess.; Intra-assay Precision (Precision within an assay): CV%<8%: Inter-assay Precision (Precision between assays): CV%<10%; 1: Three samples of known concentration were tested in twenty assays to assess. |
| Linearity | headers: To assess the linearity of the assay, samples were spiked with high concentrations of human IL-1β in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.; rows: To assess the linearity of the assay, samples were spiked with high concentrations of human IL-1β in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.: To assess the linearity of the assay, samples were spiked with high concentrations of human IL-1β in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.; 2: Sample; 3: Serum(n=4); 1: 1:1; 2: Average %; 3: 103; 1: Range %; 2: 98-108; 1: 1:2; 2: Average %; 3: 99; 1: Range %; 2: 92-105; 1: 1:4; 2: Average %; 3: 97; 1: Range %; 2: 92-104; 1: 1:8; 2: Average %; 3: 93; 1: Range %; 2: 86-98 |
| Recovery | headers: The recovery of human IL-1β spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.; rows: The recovery of human IL-1β spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.: The recovery of human IL-1β spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.; 1: Sample Type; 2: Average % Recovery; 3: Range; 1: Serum (n=5); 2: 95; 3: 89-98; 1: EDTA plasma (n=4); 2: 100; 3: 95-106 |
| Typical Data | headers: These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.; rows: These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.: These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.; ; ; 1: pg/ml; 2: OD1; 3: OD2; 4: Average; 5: Corrected; 1: 150; 2: 3.049; 3: 2.934; 4: 2.991; 5: 2.864; 1: 75; 2: 2.404; 3: 2.467; 4: 2.436; 5: 2.308; 1: 37.5; 2: 1.507; 3: 1.528; 4: 1.517; 5: 1.390; 1: 18.75; 2: 0.845; 3: 0.829; 4: 0.837; 5: 0.710; 1: 9.375; 2: 0.475; 3: 0.451; 4: 0.463; 5: 0.335; 1: 4.688; 2: 0.300; 3: 0.293; 4: 0.296; 5: 0.169; 1: 2.344; 2: 0.221; 3: 0.214; 4: 0.218; 5: 0.090; 1: 0; 2: 0.130; 3: 0.125; 4: 0.128 |
| ELISA Data Analysis | ELISA Standard Curve & Curve Expert software |
| Shelf Life | 6 months |
| Description | This ELISA kit provides quantitative measurement of interleukin-1β (IL-1β) in human samples, supporting research into innate immune activation, inflammasome biology, and inflammatory disease pathogenesis. Because IL-1β is secreted at low picogram-level concentrations and its detection must span both homeostatic and acutely inflamed states, the kit is engineered to address the dynamic range and sensitivity demands specific to this cytokine. Detection Range: The 2.344–150 pg/ml range captures IL-1β concentrations from baseline circulating levels in healthy serum (~1–5 pg/ml) through the elevated concentrations observed in sepsis, autoinflammatory syndromes, and stimulated monocyte supernatants, minimizing the need for sample dilution or concentration in most experimental contexts. Sensitivity: At 0.586 pg/ml, the kit detects sub-physiological IL-1β levels, enabling measurement in unstimulated cell culture supernatants, early-phase inflammatory responses, and plasma samples where mature IL-1β secretion is limited prior to full inflammasome activation. Sample Compatibility: Validation across serum, plasma, tissue homogenates, and cell culture supernatants allows researchers to correlate systemic IL-1β levels with local tissue production and in vitro inflammasome activation studies within a single assay platform. Assay Efficiency: Requiring only 50–100 µl per well and 1–5 hours to complete, the kit accommodates limited-volume samples from primary human macrophage cultures and supports same-day data acquisition alongside parallel NLRP3 or caspase-1 activity assays. This kit is particularly suited for studies in CAPS and other autoinflammatory disorders, sepsis immunophenotyping, and NLRP3 inflammasome pathway characterization. |
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