Recombinant Human C-C motif chemokine 2 protein (CCL2) (Active) CSB-AP000821HU CUSABIO
$142.00
In stock
SKU
CSB-AP000821HU
Product Details
| Uniprot No. | P13500 |
| Alternative Names | C-C motif chemokine 2; CCL2; CCL2_HUMAN; Chemokine (C C motif) ligand 2; GDCF 2; GDCF-2; GDCF2; HC11; HSMCR30; JE; MCAF; MCP 1; MCP-1; MCP1; MGC9434; Monocyte chemoattractant protein 1; Monocyte chemotactic and activating factor; Monocyte chemotactic protein 1; Monocyte secretory protein JE; SCYA2; Small inducible cytokine A2 (monocyte chemotactic protein 1; homologous to mouse Sig je); Small inducible cytokine A2; Small inducible cytokine subfamily A (Cys Cys); member 2; Small-inducible cytokine A2; SMC CF; SMC-CF; SMCCF |
| Species | Homo sapiens (Human) |
| Source | E.coli |
| Expression Region | 24-99aa |
| Purity | >96% as determined by SDS-PAGE. |
| Endotoxin | Less than 1.0 EU/μg as determined by LAL method. |
| Activity | Fully biologically active when compared to standard. The biological activity determined by a chemotaxis bioassay using human monocytes is in a concentration range of 10-100 ng/ml. |
| Tag Info | Tag-Free |
| Form | Lyophilized powder |
| Buffer | Lyophilized from a 0.2 µm filtered PBS, pH 7.4 |
| Reconstitution | We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. Our default final concentration of glycerol is 50%. Customers could use it as reference. |
| Troubleshooting and FAQs | Protein FAQs |
| Notes | Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week. |
| Lead Time | 5-10 business days |
| Target Names | CCL2 |
| Research Area | Immunology |
| Complete Sequence | QPDAINAPVT CCYNFTNRKI SVQRLASYRR ITSSKCPKEA VIFKTIVAKE ICADPKQKWV QDSMDHLDKQ TQTPKT |
| Mol. Weight | 8.7 kDa |
| Protein Length | Full Length of Mature Protein |
| Storage Condition | Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles. |
| Shelf Life | The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C. |
| Datasheet & COA | Please contact us to get it. |
| Description | CCL2 (MCP-1) is a pivotal monocyte chemoattractant that drives innate immune cell recruitment during inflammation, making validated bioactivity essential for meaningful functional studies. This tag-free recombinant human CCL2, encompassing the full-length mature sequence (residues 24–99), induces directed monocyte migration in chemotaxis bioassays at 10–100 ng/ml, confirming signaling potency through CCR2-dependent pathways suitable for Transwell migration assays, NF-κB and MAPK pathway activation studies, and in vivo inflammation or tumor biology models. Endotoxin levels below 1.0 EU/μg minimize LPS-driven artifacts that commonly confound monocyte and macrophage functional readouts, which supports use in sensitive cell-based activation and differentiation experiments as well as serving as a reliable reference standard in cytokine detection ELISAs. Produced in *E. coli* with >96% purity by SDS-PAGE, this protein also provides a suitable basis for antibody development workflows including immunization, coating antigen preparation, and Western blot positive controls. |
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