Human Interleukin 12,IL-12/P70 ELISA Kit CSB-E04599h-IS CUSABIO
$50.00
In stock
SKU
CSB-E04599h-IS
Product Details
| Species | Homo sapiens (Human) |
| Storage | Store at 2-8°C. Please refer to protocol. |
| Sensitivity | 0.975 pg/ml |
| Troubleshooting and FAQs | ELISA kit FAQs |
| Lead Time | 3-5 working days after you place the order, and it takes another 3-5 days for delivery via DHL or FedEx. |
| Abbreviation | IL-12/P70 |
| Sample Types | serum, plasma, cell culture supernates |
| Detection Range | 3.9 pg/ml-250 pg/ml |
| Assay Time | 1-5h |
| Sample Loading Volume | 50-100ul |
| Detection Wavelength | 450 nm |
| Research Area | Immunology |
| Assay Principle | quantitative |
| Measurement | Sandwich |
| Precision | headers: Intra-assay Precision (Precision within an assay): CV%<8%; rows: Intra-assay Precision (Precision within an assay): CV%<8%: Intra-assay Precision (Precision within an assay): CV%<8%; 1: Three samples of known concentration were tested twenty times on one plate to assess.; Intra-assay Precision (Precision within an assay): CV%<8%: Inter-assay Precision (Precision between assays): CV%<10%; 1: Three samples of known concentration were tested in twenty assays to assess. |
| Linearity | headers: To assess the linearity of the assay, samples were spiked with high concentrations of human IL-12/P70 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.; rows: To assess the linearity of the assay, samples were spiked with high concentrations of human IL-12/P70 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.: To assess the linearity of the assay, samples were spiked with high concentrations of human IL-12/P70 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.; 2: Sample; 3: Serum(n=4); 1: 1:1; 2: Average %; 3: 104; 1: Range %; 2: 97-111; 1: 1:2; 2: Average %; 3: 92; 1: Range %; 2: 85-99; 1: 1:4; 2: Average %; 3: 96; 1: Range %; 2: 90-101; 1: 1:8; 2: Average %; 3: 90; 1: Range %; 2: 83-97 |
| Recovery | headers: The recovery of human IL-12/P70 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.; rows: The recovery of human IL-12/P70 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.: The recovery of human IL-12/P70 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.; 1: Sample Type; 2: Average % Recovery; 3: Range; 1: Serum (n=5); 2: 98; 3: 91-104; 1: EDTA plasma (n=4); 2: 92; 3: 85-99 |
| Typical Data | headers: These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.; rows: These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.: These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.; ; ; 1: pg/ml; 2: OD1; 3: OD2; 4: Average; 5: Corrected; 1: 250; 2: 2.333; 3: 2.434; 4: 2.383; 5: 2.196; 1: 125; 2: 1.229; 3: 1.297; 4: 1.263; 5: 1.075; 1: 62.5; 2: 0.853; 3: 0.828; 4: 0.840; 5: 0.652; 1: 31.25; 2: 0.571; 3: 0.559; 4: 0.565; 5: 0.377; 1: 15.6; 2: 0.345; 3: 0.358; 4: 0.351; 5: 0.164; 1: 7.8; 2: 0.287; 3: 0.273; 4: 0.280; 5: 0.092; 1: 3.9; 2: 0.240; 3: 0.238; 4: 0.239; 5: 0.051; 1: 0; 2: 0.191; 3: 0.185; 4: 0.188 |
| Materials provided | A micro ELISA plate ---The 96-well plate has been pre-coated with an anti-human IL-12 antibody. This dismountable microplate can be divided into 12 x 8 strip plates. Two vials lyophilized standard ---Dilute a bottle of the standard at dilution series, read the OD values, and then draw a standard curve. One vial Biotin-labeled IL-12 antibody (100 x concentrate) (120 μl/bottle) ---Act as the detection antibody. One vial HRP-avidin (100 x concentrate) (120 μl/bottle) ---Bind to the detection antibody and react with the TMB substrate to make the solution chromogenic. One vial Biotin-antibody Diluent (15 ml/bottle) ---Dilute the Biotin-antibody. One vial HRP-avidin Diluent (15 ml/bottle) ---Dilute the HRP-avidin solution. One vial Sample Diluent (50 ml/bottle)---Dilute the sample to an appropriate concentration. One vial Wash Buffer (25 x concentrate) (20 ml/bottle) ---Wash away unbound or free substances. One vial TMB Substrate (10 ml/bottle) ---Act as the chromogenic agent. TMB interacts with HRP, eliciting the solution turns blue. One vial Stop Solution (10 ml/bottle) ---Stop the color reaction. The solution color immediately turns from blue to yellow. Four Adhesive Strips (For 96 wells) --- Cover the microplate when incubation. An instruction manual |
| Materials not provided | A microplate reader capable of measuring absorbance at 450 nm, with the correction wavelength set at 540 nm or 570 nm. An incubator can provide stable incubation conditions up to 37°C±5°C. Centrifuge Vortex Squirt bottle, manifold dispenser, or automated microplate washer Absorbent paper for blotting the microtiter plate 50-300ul multi-channel micropipette Pipette tips Single-channel micropipette with different ranges 100ml and 500ml graduated cylinders Deionized or distilled water Timer Test tubes for dilution |
| ELISA Data Analysis | ELISA Standard Curve & Curve Expert software |
| Shelf Life | 6 months |
| Description | This ELISA kit provides quantitative measurement of human IL-12/p70, the bioactive heterodimer (p35/p40) critical for Th1 polarization and IFN-γ induction, in serum, plasma, and cell culture supernatants. Because IL-12/p70 circulates at very low concentrations and is often the rate-limiting signal in Th1 commitment studies, precise detection at the picogram level is essential for meaningful data. Detection Range: The 3.9–250 pg/ml range spans both the low baseline levels of IL-12/p70 found in healthy human serum (typically <5–20 pg/ml) and the elevated concentrations observed during active infection or following dendritic cell stimulation in vitro, minimizing the need for sample dilution or concentration in most experimental contexts. Sensitivity: At 0.975 pg/ml, this kit detects IL-12/p70 in unstimulated or weakly stimulated conditions—critical for capturing early dendritic cell activation kinetics or distinguishing subtle differences in monocyte-derived IL-12 production between patient cohorts. Sample Compatibility: Validation across serum, plasma, and cell culture supernatants enables direct comparison between ex vivo patient cytokine levels and in vitro stimulation assays using PBMCs or monocyte-derived dendritic cells within a single study. Assay Efficiency: The 1–5 hour protocol requiring only 50–100 µl accommodates limited clinical specimens and small-scale primary cell cultures where IL-12/p70 output per well is constrained by low cell yields. This kit supports research in sepsis immunopathology, vaccine adjuvant development, and Th1-mediated autoimmune disease profiling. |
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