Human Interleukin 13,IL-13 ELISA KIT CSB-E04601h-IS CUSABIO
$50.00
In stock
SKU
CSB-E04601h-IS
Product Details
| Uniprot No. | P35225 |
| Species | Homo sapiens (Human) |
| Storage | Store at 2-8°C. Please refer to protocol. |
| Sensitivity | 0.78 pg/mL |
| Troubleshooting and FAQs | ELISA kit FAQs |
| Lead Time | 3-5 working days after you place the order, and it takes another 3-5 days for delivery via DHL or FedEx. |
| Associated names | Allergic rhinitis ELISA Kit; ALRH ELISA Kit; BHR 1 ELISA Kit; BHR1 ELISA Kit; Bronchial hyperresponsiveness 1 (bronchial asthma) ELISA Kit; IL 13 ELISA Kit; IL-13 ELISA Kit; Il13 ELISA Kit; IL13_HUMAN ELISA Kit; Interleukin 13 ELISA Kit; Interleukin-13 ELISA Kit; interleukin13 ELISA Kit; MGC116786 ELISA Kit; MGC116788 ELISA Kit; MGC116789 ELISA Kit; NC 30 ELISA Kit; NC30 ELISA Kit; P 600 ELISA Kit; P600 ELISA Kit |
| Abbreviation | IL13 |
| Sample Types | serum, plasma, tissue homogenates, cell culture supernates |
| Detection Range | 3.12 pg/mL-200 pg/mL |
| Assay Time | 1-5h |
| Sample Loading Volume | 50-100ul |
| Detection Wavelength | 450 nm |
| Research Area | Immunology |
| Assay Principle | quantitative |
| Measurement | Sandwich |
| Precision | headers: Intra-assay Precision (Precision within an assay): CV%<8%; rows: Intra-assay Precision (Precision within an assay): CV%<8%: Intra-assay Precision (Precision within an assay): CV%<8%; 1: Three samples of known concentration were tested twenty times on one plate to assess.; Intra-assay Precision (Precision within an assay): CV%<8%: Inter-assay Precision (Precision between assays): CV%<10%; 1: Three samples of known concentration were tested in twenty assays to assess. |
| Linearity | headers: To assess the linearity of the assay, samples were spiked with high concentrations of human IL-13 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.; rows: To assess the linearity of the assay, samples were spiked with high concentrations of human IL-13 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.: To assess the linearity of the assay, samples were spiked with high concentrations of human IL-13 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.; 2: Sample; 3: Serum(n=4); 1: 1:1; 2: Average %; 3: 87; 1: Range %; 2: 82-94; 1: 1:2; 2: Average %; 3: 95; 1: Range %; 2: 88-101; 1: 1:4; 2: Average %; 3: 92; 1: Range %; 2: 87-98; 1: 1:8; 2: Average %; 3: 100; 1: Range %; 2: 95-105 |
| Recovery | headers: The recovery of human IL-13 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.; rows: The recovery of human IL-13 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.: The recovery of human IL-13 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.; 1: Sample Type; 2: Average % Recovery; 3: Range; 1: Serum (n=5); 2: 105; 3: 97-111; 1: EDTA plasma (n=4); 2: 96; 3: 90-100 |
| Typical Data | headers: These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.; rows: These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.: These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.; ; ; 1: pg/ml; 2: OD1; 3: OD2; 4: Average; 5: Corrected; 1: 200; 2: 3.074; 3: 2.934; 4: 3.004; 5: 2.879; 1: 100; 2: 2.111; 3: 2.167; 4: 2.139; 5: 2.015; 1: 50; 2: 1.264; 3: 1.328; 4: 1.296; 5: 1.172; 1: 25; 2: 0.793; 3: 0.819; 4: 0.806; 5: 0.682; 1: 12.5; 2: 0.413; 3: 0.421; 4: 0.417; 5: 0.293; 1: 6.25; 2: 0.263; 3: 0.253; 4: 0.258; 5: 0.134; 1: 3.12; 2: 0.169; 3: 0.174; 4: 0.171; 5: 0.047; 1: 0; 2: 0.124; 3: 0.125; 4: 0.124 |
| ELISA Data Analysis | ELISA Standard Curve & Curve Expert software |
| Shelf Life | 6 months |
| Description | This ELISA kit provides quantitative measurement of interleukin-13 (IL-13) in human samples, supporting research into Th2-mediated immune responses, allergic inflammation, and tissue fibrosis. Because circulating IL-13 is typically present at low picogram-per-milliliter concentrations and rises modestly even during active disease, precise detection within a narrow dynamic window is essential for meaningful data. Detection Range: The 3.12–200 pg/mL range aligns with reported IL-13 levels in human serum and plasma, which rarely exceed low double-digit pg/mL at baseline and may reach the tens-of-pg/mL range during allergic exacerbation or helminth infection. Most samples can be run neat or with minimal dilution, simplifying assay planning for clinical and translational studies. Sensitivity: At 0.78 pg/mL, the kit resolves the characteristically low basal IL-13 concentrations found in healthy donor serum and unstimulated cell culture supernates, where this cytokine often falls below 5 pg/mL. Sample Compatibility: Validation across serum, plasma, tissue homogenates, and cell culture supernates enables researchers to correlate systemic IL-13 levels with local tissue expression and in vitro Th2 polarization or mast cell stimulation outputs within a single assay platform. Assay Efficiency: The 50–100 µL sample volume and 1–5 hour protocol accommodate limited-volume specimens from bronchoscopy lavage fractions or pediatric blood draws while supporting same-day results. This kit is directly applicable to studies of asthma pathogenesis, atopic dermatitis biomarker profiling, and idiopathic pulmonary fibrosis. |
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