Human Interleukin 1β,IL-1β ELISA Kit CSB-E08053h CUSABIO
$50.00
In stock
SKU
CSB-E08053h
Product Details
| Uniprot No. | P01584 |
| Species | Homo sapiens (Human) |
| Storage | Store at 2-8°C. Please refer to protocol. |
| Sensitivity | 1.95 pg/ml |
| Troubleshooting and FAQs | ELISA kit FAQs |
| Lead Time | 3-5 working days after you place the order, and it takes another 3-5 days for delivery via DHL or FedEx. |
| Associated names | Catabolin ELISA Kit; H1 ELISA Kit; IFN beta inducing factor ELISA Kit; IL 1 ELISA Kit; IL 1 beta ELISA Kit; IL-1 beta ELISA Kit; IL1 ELISA Kit; IL1 BETA ELISA Kit; IL1B ELISA Kit; IL1B_HUMAN ELISA Kit; IL1F2 ELISA Kit; Interleukin 1 beta ELISA Kit; Interleukin 1 beta precursor ELISA Kit; interleukin 1; beta ELISA Kit; Interleukin-1 beta ELISA Kit; OAF ELISA Kit; Osteoclast activating factor ELISA Kit; OTTHUMP00000162031 ELISA Kit; Preinterleukin 1 beta ELISA Kit; Preinterleukin beta ELISA Kit; Pro interleukin 1 beta ELISA Kit |
| Abbreviation | IL1B |
| Sample Types | serum, plasma, tissue homogenates |
| Detection Range | 7.8 pg/ml - 500 pg/ml |
| Assay Time | 1-5h |
| Sample Loading Volume | 50-100ul |
| Detection Wavelength | 450 nm |
| Research Area | Immunology |
| Assay Principle | quantitative |
| Measurement | Sandwich |
| Precision | headers: Intra-assay Precision (Precision within an assay): CV%<8%; rows: Intra-assay Precision (Precision within an assay): CV%<8%: Intra-assay Precision (Precision within an assay): CV%<8%; 1: Three samples of known concentration were tested twenty times on one plate to assess.; Intra-assay Precision (Precision within an assay): CV%<8%: Inter-assay Precision (Precision between assays): CV%<10%; 1: Three samples of known concentration were tested in twenty assays to assess. |
| Linearity | headers: To assess the linearity of the assay, samples were spiked with high concentrations of human IL-1β in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.; rows: To assess the linearity of the assay, samples were spiked with high concentrations of human IL-1β in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.: To assess the linearity of the assay, samples were spiked with high concentrations of human IL-1β in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.; 2: Sample; 3: Serum(n=4); 1: 1:1; 2: Average %; 3: 90; 1: Range %; 2: 80-95; 1: 1:2; 2: Average %; 3: 96; 1: Range %; 2: 91-105; 1: 1:4; 2: Average %; 3: 107; 1: Range %; 2: 103-112; 1: 1:8; 2: Average %; 3: 93; 1: Range %; 2: 86-98 |
| Recovery | headers: The recovery of human IL-1β spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.; rows: The recovery of human IL-1β spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.: The recovery of human IL-1β spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.; 1: Sample Type; 2: Average % Recovery; 3: Range; 1: Serum (n=5); 2: 95; 3: 89-98; 1: EDTA plasma (n=4); 2: 92; 3: 88-96 |
| Typical Data | headers: These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.; rows: These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.: These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.; ; ; 1: pg/ml; 2: OD1; 3: OD2; 4: Average; 5: Corrected; 1: 500; 2: 2.439; 3: 2.604; 4: 2.522; 5: 2.343; 1: 250; 2: 1.493; 3: 1.467; 4: 1.480; 5: 1.301; 1: 125; 2: 0.864; 3: 0.858; 4: 0.861; 5: 0.683; 1: 62.5; 2: 0.566; 3: 0.589; 4: 0.578; 5: 0.399; 1: 31.2; 2: 0.378; 3: 0.361; 4: 0.370; 5: 0.191; 1: 15.6; 2: 0.309; 3: 0.293; 4: 0.301; 5: 0.122; 1: 7.8; 2: 0.267; 3: 0.254; 4: 0.261; 5: 0.082; 1: 0; 2: 0.182; 3: 0.175; 4: 0.179 |
| ELISA Data Analysis | ELISA Standard Curve & Curve Expert software |
| Shelf Life | 6 months |
| Description | The human IL1B ELISA Kit is intended to measure human IL1B in serum, plasma, or tissue homogenates. This assay uses the bi-antibody sandwich technique and enzyme-substrate chromogenic reaction to quantify human IL1B concentration. The intensity of the colored product is directly proportional to the concentration of IL1B present in the sample. This human IL1B ELISA kit has high specificity and excellent sensitivity and has been described in more than 83 publications. IL1B is a potent pro-inflammatory cytokine that plays a prominent role in the host defense against infections, as well as in tissue homeostasis and repair. IL-1B binds to its receptor and activates a cascade of downstream signaling pathways, including the MAPK, cyclooxygenase, and NK–kB pathways, resulting in macrophage activation, intratumoral accumulation of immunosuppressive myeloid cells, and tumor growth, invasiveness, metastasis, and angiogenesis. Abnormal IL1B signaling drives tumorigenesis by promoting epithelial-to-mesenchymal transition, increasing the production of inflammatory cytokines and chemokines, inducing immune repression, and apoptosis resistance, as well as increasing leukocyte adhesions. Aberrant activation of IL1B is related to poor prognosis in most cancer types, including colon, lung, and breast cancer. IL1B-causing inflammation occurs in several disease states, including diabetes, arthritis, and atherosclerosis. |
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