Human Interleukin-36 alpha(IL1F6) ELISA kit CSB-EL011617HU CUSABIO
$50.00
In stock
SKU
CSB-EL011617HU
Product Details
| Target Name | interleukin 1 family, member 6 (epsilon) |
| Uniprot No. | Q9UHA7 |
| Species | Homo sapiens (Human) |
| Storage | Store at 2-8°C. Please refer to protocol. |
| Sensitivity | 3.9 pg/mL |
| Troubleshooting and FAQs | ELISA kit FAQs |
| Lead Time | 3-5 working days after you place the order, and it takes another 3-5 days for delivery via DHL or FedEx. |
| Associated names | FIL1 ELISA Kit; FIL1 epsilon ELISA Kit; FIL1(EPSILON) ELISA Kit; FIL1E ELISA Kit; IL 1 epsilon ELISA Kit; IL 1F6 ELISA Kit; IL 1H1 ELISA Kit; IL-1 epsilon ELISA Kit; IL-1F6 ELISA Kit; IL1E ELISA Kit; IL1F6 ELISA Kit; IL1F6_HUMAN ELISA Kit; IL1RP2 ELISA Kit; IL36 alpha ELISA Kit; IL36A ELISA Kit; Interleukin 1 epsilon ELISA Kit; Interleukin 1 family member 6 (epsilon) ELISA Kit; Interleukin 1 family member 6 ELISA Kit; Interleukin 36 alpha ELISA Kit; Interleukin-1 epsilon ELISA Kit; Interleukin-1 family member 6 ELISA Kit; MGC129552 ELISA Kit; MGC129553 ELISA Kit; MGC151479 ELISA Kit; MGC151481 ELISA Kit; OTTMUSP00000012798 ELISA Kit; RP23-176J12.4 ELISA Kit |
| Abbreviation | IL36A |
| Sample Types | serum, plasma, tissue homogenates, cell culture supernates |
| Detection Range | 15.6 pg/mL-1000 pg/mL |
| Assay Time | 1-5h |
| Sample Loading Volume | 50-100ul |
| Detection Wavelength | 450 nm |
| Research Area | Immunology |
| Assay Principle | quantitative |
| Measurement | Sandwich |
| Precision | headers: Intra-assay Precision (Precision within an assay): CV%<8%; rows: Intra-assay Precision (Precision within an assay): CV%<8%: Intra-assay Precision (Precision within an assay): CV%<8%; 1: Three samples of known concentration were tested twenty times on one plate to assess.; Intra-assay Precision (Precision within an assay): CV%<8%: Inter-assay Precision (Precision between assays): CV%<10%; 1: Three samples of known concentration were tested in twenty assays to assess.; |
| Linearity | headers: To assess the linearity of the assay, samples were spiked with high concentrations of human IL1F6 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.; rows: To assess the linearity of the assay, samples were spiked with high concentrations of human IL1F6 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.: To assess the linearity of the assay, samples were spiked with high concentrations of human IL1F6 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.; 2: Sample; 3: Serum(n=4); 1: 1:1; 2: Average %; 3: 99; 1: Range %; 2: 94-103; 1: 1:2; 2: Average %; 3: 87; 1: Range %; 2: 80-95; 1: 1:4; 2: Average %; 3: 95; 1: Range %; 2: 91-99; 1: 1:8; 2: Average %; 3: 93; 1: Range %; 2: 86-98 |
| Recovery | headers: The recovery of human IL1F6 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.; rows: The recovery of human IL1F6 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.: The recovery of human IL1F6 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.; 1: Sample Type; 2: Average % Recovery; 3: Range; 1: Serum (n=5); 2: 92; 3: 86-98; 1: EDTA plasma (n=4); 2: 97; 3: 92-102; ; |
| Typical Data | headers: These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.; rows: These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.: These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.; 1: pg/ml OD1 OD2 Average Corrected 1000 1.966 1.957 1.962 1.861 500 1.432 1.501 1.467 1.366 250 0.950 0.920 0.935 0.834 125 0.680 0.689 0.685 0.584 62.5 0.465 0.502 0.484 0.383 31.2 0.275 0.287 0.281 0.180 15.6 0.182 0.178 0.180 0.079 0 0.098 0.103 0.101; 2: pg/ml; 3: OD1; 4: OD2; 5: Average; 6: Corrected; 8: 1000; 9: 1.966; 10: 1.957; 11: 1.962; 12: 1.861; 14: 500; 15: 1.432; 16: 1.501; 17: 1.467; 18: 1.366; 20: 250; 21: 0.950; 22: 0.920; 23: 0.935; 24: 0.834; 26: 125; 27: 0.680; 28: 0.689; 29: 0.685; 30: 0.584; 32: 62.5; 33: 0.465; 34: 0.502; 35: 0.484; 36: 0.383; 38: 31.2; 39: 0.275; 40: 0.287; 41: 0.281; 42: 0.180; 44: 15.6; 45: 0.182; 46: 0.178; 47: 0.180; 48: 0.079; 50: 0; 51: 0.098; 52: 0.103; 53: 0.101; 1: pg/ml; 2: OD1; 3: OD2; 4: Average; 5: Corrected; 1: 1000; 2: 1.966; 3: 1.957; 4: 1.962; 5: 1.861; 1: 500; 2: 1.432; 3: 1.501; 4: 1.467; 5: 1.366; 1: 250; 2: 0.950; 3: 0.920; 4: 0.935; 5: 0.834; 1: 125; 2: 0.680; 3: 0.689; 4: 0.685; 5: 0.584; 1: 62.5; 2: 0.465; 3: 0.502; 4: 0.484; 5: 0.383; 1: 31.2; 2: 0.275; 3: 0.287; 4: 0.281; 5: 0.180; 1: 15.6; 2: 0.182; 3: 0.178; 4: 0.180; 5: 0.079; 1: 0; 2: 0.098; 3: 0.103; 4: 0.101 |
| ELISA Data Analysis | ELISA Standard Curve & Curve Expert software |
| Shelf Life | 6 months |
| Description | The human interleukin-36 alpha (IL36A) ELISA kit is suitable for quantitatively determining the human IL36A complex in multiple samples, including serum, plasma, tissue homogenates, or cell culture supernates. This assay employs the bi-antibody sandwich technique and enzyme-substrate chromogenic reaction to quantify human IL36A levels in the sample. The amount of synthesized colored product is positively related to the analyte of interest in the sample. IL36A, also called IL1F6, is expressed during embryonic development and is highly abundant in epithelial cells, as well as monocytes, B cells, and T cells. IL36A has been shown to exert strong pro-inflammatory effects in the lungs of mice. Along with its modulation by EGF, IL36A plays an important role in skin barrier function and homeostasis. Ichii et al. indicated an association between intrarenal IL36A overexpression and the progression of tubulointerstitial inflammations and morpho-functional changes of distal tubule (DT) epithelial cells. IL36A may be a novel kidney injury marker useful for evaluating DT damages. |
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