Human Macrophage Inflammatory Protein-1α,MIP-1α ELISA kit CSB-E04662h CUSABIO
$50.00
In stock
SKU
CSB-E04662h
Product Details
| Uniprot No. | P10147 |
| Species | Homo sapiens (Human) |
| Storage | Store at 2-8°C. Please refer to protocol. |
| Sensitivity | 9.75 pg/ml |
| Troubleshooting and FAQs | ELISA kit FAQs |
| Lead Time | 3-5 working days after you place the order, and it takes another 3-5 days for delivery via DHL or FedEx. |
| Associated names | C C motif chemokine 3 ELISA Kit; CCL 3 ELISA Kit; CCL3 ELISA Kit; CCL3_HUMAN ELISA Kit; Chemokine (C C motif) ligand 3 ELISA Kit; Chemokine C C motif ligand 3 ELISA Kit; Chemokine ligand 3 ELISA Kit; G0/G1 switch regulatory protein 19 1 ELISA Kit; G0/G1 switch regulatory protein 19-1 ELISA Kit; G0S19 1 ELISA Kit; G0S19 1 protein ELISA Kit; Heparin binding chemotaxis protein ELISA Kit; L2G25B ELISA Kit; LD78 alpha ELISA Kit; LD78-alpha(4-69) ELISA Kit; LD78alpha ELISA Kit; Macrophage inflammatory protein 1 alpha ELISA Kit; Macrophage inflammatory protein 1-alpha ELISA Kit; macrophage inflammatory protein 1a ELISA Kit; MIP 1 alpha ELISA Kit; MIP 1A ELISA Kit; MIP-1-alpha ELISA Kit; MIP-1-alpha(4-69) ELISA Kit; MIP1 alpha ELISA Kit; MIP1A ELISA Kit; PAT 464.1 ELISA Kit; SCYA 3 ELISA Kit; SCYA3 ELISA Kit; SIS alpha ELISA Kit; SIS beta ELISA Kit; SIS-beta ELISA Kit; Small inducible cytokine A3 ELISA Kit; small inducible cytokine A3 (homologous to mouse Mip-1a) ELISA Kit; Small-inducible cytokine A3 ELISA Kit; Tonsillar lymphocyte LD78 alpha protein ELISA Kit |
| Abbreviation | CCL3 |
| Sample Types | serum, plasma, tissue homogenates |
| Detection Range | 39 pg/ml - 2500 pg/ml |
| Assay Time | 1-5h |
| Sample Loading Volume | 50-100ul |
| Detection Wavelength | 450 nm |
| Research Area | Immunology |
| Assay Principle | quantitative |
| Measurement | Sandwich |
| Precision | headers: Intra-assay Precision (Precision within an assay): CV%<8%; rows: Intra-assay Precision (Precision within an assay): CV%<8%: Intra-assay Precision (Precision within an assay): CV%<8%; 1: Three samples of known concentration were tested twenty times on one plate to assess.; Intra-assay Precision (Precision within an assay): CV%<8%: Inter-assay Precision (Precision between assays): CV%<10%; 1: Three samples of known concentration were tested in twenty assays to assess. |
| Linearity | headers: To assess the linearity of the assay, samples were spiked with high concentrations of human MIP-1α in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.; rows: To assess the linearity of the assay, samples were spiked with high concentrations of human MIP-1α in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.: To assess the linearity of the assay, samples were spiked with high concentrations of human MIP-1α in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.; 2: Sample; 3: Serum(n=4); 1: 1:1; 2: Average %; 3: 86; 1: Range %; 2: 81-90; 1: 1:2; 2: Average %; 3: 99; 1: Range %; 2: 94-102; 1: 1:4; 2: Average %; 3: 99; 1: Range %; 2: 97-101; 1: 1:8; 2: Average %; 3: 94; 1: Range %; 2: 90-98 |
| Recovery | headers: The recovery of human MIP-1α spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.; rows: The recovery of human MIP-1α spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.: The recovery of human MIP-1α spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.; 1: Sample Type; 2: Average % Recovery; 3: Range; 1: Serum (n=5); 2: 93; 3: 89-96; 1: EDTA plasma (n=4); 2: 96; 3: 94-99 |
| Typical Data | headers: These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.; rows: These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.: These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.; ; ; 1: pg/ml; 2: OD1; 3: OD2; 4: Average; 5: Corrected; 1: 2500; 2: 2.483; 3: 2.468; 4: 2.476; 5: 2.372; 1: 1250; 2: 1.999; 3: 1.923; 4: 1.961; 5: 1.857; 1: 625; 2: 1.256; 3: 1.286; 4: 1.271; 5: 1.167; 1: 312.5; 2: 0.639; 3: 0.691; 4: 0.665; 5: 0.561; 1: 156.25; 2: 0.424; 3: 0.439; 4: 0.432; 5: 0.328; 1: 78; 2: 0.292; 3: 0.279; 4: 0.286; 5: 0.182; 1: 39; 2: 0.196; 3: 0.187; 4: 0.192; 5: 0.088; 1: 0; 2: 0.105; 3: 0.103; 4: 0.104 |
| ELISA Data Analysis | ELISA Standard Curve & Curve Expert software |
| Shelf Life | 6 months |
| Description | The product CSB-E04662h is a sandwich ELISA kit developed to measure concentrations of human macrophage inflammatory protein-1α (MIP-1α) in serum, plasma, or tissue homogenates. This assay uses the sandwich enzyme immunoassay technique in combination with the enzyme-substrate chromogenic reaction to quantify the analyte in the sample. The color develops positively to the amount of MIP-1α in samples. The color intensity is measured at 450 nm via a microplate reader. MIP-1α, also called CCL3, is a pro-inflammatory chemokine expressed constitutively in the bone marrow, including by osteoblasts. It is induced during inflammation. CCL3 is a chemotactic cytokine crucial for inflammatory cell recruitment in homeostatic and pathological conditions. In addition to its proinflammatory activities, CCL3 negatively regulates the proliferation of hematopoietic stem/progenitor cells (HSPCs) in the bone marrow (BM). CCL3 and its receptor CCR1 are reported to mediate the bone remodeling that occurs during orthodontic tooth movement, and may also regulate osteoclastogenesis in osteomyelitis. CCL3 might stimulate cancer progression by promoting leukocyte accumulation, angiogenesis, and tumor growth. |
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