Human Mitochondrial brown fat uncoupling protein 1(UCP1) ELISA kit CSB-EL025554HU CUSABIO
$50.00
In stock
SKU
CSB-EL025554HU
Product Details
| Target Name | uncoupling protein 1 (mitochondrial, proton carrier) |
| Uniprot No. | P25874 |
| Species | Homo sapiens (Human) |
| Storage | Store at 2-8°C. Please refer to protocol. |
| Sensitivity | 7.81 pg/mL |
| Troubleshooting and FAQs | ELISA kit FAQs |
| Lead Time | 3-5 working days after you place the order, and it takes another 3-5 days for delivery via DHL or FedEx. |
| Associated names | mitochondrial brown fat uncoupling protein ELISA Kit; Mitochondrial brown fat uncoupling protein 1 ELISA Kit; SLC25A7 ELISA Kit; Solute carrier family 25 member 7 ELISA Kit; Thermogenin ELISA Kit; UCP 1 ELISA Kit; UCP ELISA Kit; UCP1 ELISA Kit; UCP1_HUMAN ELISA Kit; uncoupling protein 1 (mitochondrial, proton carrier) ELISA Kit; Uncoupling protein 1 ELISA Kit |
| Abbreviation | UCP1 |
| Sample Types | serum, plasma, tissue homogenates |
| Detection Range | 31.25 pg/mL-2000 pg/mL |
| Assay Time | 1-5h |
| Sample Loading Volume | 50-100ul |
| Detection Wavelength | 450 nm |
| Research Area | Cancer |
| Assay Principle | quantitative |
| Measurement | Sandwich |
| Precision | headers: Intra-assay Precision (Precision within an assay): CV%<8%; rows: Intra-assay Precision (Precision within an assay): CV%<8%: Intra-assay Precision (Precision within an assay): CV%<8%; 1: Three samples of known concentration were tested twenty times on one plate to assess.; Intra-assay Precision (Precision within an assay): CV%<8%: Inter-assay Precision (Precision between assays): CV%<10%; 1: Three samples of known concentration were tested in twenty assays to assess.; |
| Linearity | headers: To assess the linearity of the assay, samples were spiked with high concentrations of human UCP1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.; rows: To assess the linearity of the assay, samples were spiked with high concentrations of human UCP1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.: To assess the linearity of the assay, samples were spiked with high concentrations of human UCP1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.; 2: Sample; 3: Serum(n=4); 1: 1:1; 2: Average %; 3: 92; 1: Range %; 2: 87-99; 1: 1:2; 2: Average %; 3: 101; 1: Range %; 2: 93-105; 1: 1:4; 2: Average %; 3: 94; 1: Range %; 2: 86-98; 1: 1:8; 2: Average %; 3: 98; 1: Range %; 2: 93-105 |
| Recovery | headers: The recovery of human UCP1 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.; rows: The recovery of human UCP1 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.: The recovery of human UCP1 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.; 1: Sample Type; 2: Average % Recovery; 3: Range; 1: Serum (n=5); 2: 95; 3: 90-98; 1: EDTA plasma (n=4); 2: 92; 3: 89-95; ; |
| Typical Data | headers: These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.; rows: These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.: These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.; 1: pg/ml OD1 OD2 Average Corrected 2000 2.093 1.952 2.023 1.940 1000 1.344 1.321 1.333 1.250 500 0.781 0.777 0.779 0.696 250 0.471 0.455 0.463 0.380 125 0.283 0.278 0.281 0.198 62.5 0.212 0.207 0.210 0.127 31.25 0.149 0.145 0.147 0.064 0 0.085 0.081 0.083; 2: pg/ml; 3: OD1; 4: OD2; 5: Average; 6: Corrected; 8: 2000; 9: 2.093; 10: 1.952; 11: 2.023; 12: 1.940; 14: 1000; 15: 1.344; 16: 1.321; 17: 1.333; 18: 1.250; 20: 500; 21: 0.781; 22: 0.777; 23: 0.779; 24: 0.696; 26: 250; 27: 0.471; 28: 0.455; 29: 0.463; 30: 0.380; 32: 125; 33: 0.283; 34: 0.278; 35: 0.281; 36: 0.198; 38: 62.5; 39: 0.212; 40: 0.207; 41: 0.210; 42: 0.127; 44: 31.25; 45: 0.149; 46: 0.145; 47: 0.147; 48: 0.064; 50: 0; 51: 0.085; 52: 0.081; 53: 0.083; 1: pg/ml; 2: OD1; 3: OD2; 4: Average; 5: Corrected; 1: 2000; 2: 2.093; 3: 1.952; 4: 2.023; 5: 1.940; 1: 1000; 2: 1.344; 3: 1.321; 4: 1.333; 5: 1.250; 1: 500; 2: 0.781; 3: 0.777; 4: 0.779; 5: 0.696; 1: 250; 2: 0.471; 3: 0.455; 4: 0.463; 5: 0.380; 1: 125; 2: 0.283; 3: 0.278; 4: 0.281; 5: 0.198; 1: 62.5; 2: 0.212; 3: 0.207; 4: 0.210; 5: 0.127; 1: 31.25; 2: 0.149; 3: 0.145; 4: 0.147; 5: 0.064; 1: 0; 2: 0.085; 3: 0.081; 4: 0.083 |
| ELISA Data Analysis | ELISA Standard Curve & Curve Expert software |
| Shelf Life | 6 months |
| Description | This Human UCP1 ELISA Kit was designed for the quantitative measurement of Human UCP1 protein in serum, plasma, tissue homogenates. It is a Sandwich ELISA kit, its detection range is 31.25 pg/mL-2000 pg/mL and the sensitivity is 7.81 pg/mL . |
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