Human Myostatin,MSTN ELISA Kit CSB-E11300h CUSABIO
$50.00
In stock
SKU
CSB-E11300h
Product Details
| Target Name | myostatin |
| Uniprot No. | O14793 |
| Species | Homo sapiens (Human) |
| Storage | Store at 2-8°C. Please refer to protocol. |
| Sensitivity | 0.312 ng/mL |
| Troubleshooting and FAQs | ELISA kit FAQs |
| Lead Time | 3-5 working days after you place the order, and it takes another 3-5 days for delivery via DHL or FedEx. |
| Associated names | Cmpt ELISA Kit; GDF-8 ELISA Kit; GDF8_HUMAN ELISA Kit; Growth/differentiation factor 8 ELISA Kit; MSLHP ELISA Kit; MSTN ELISA Kit; MSTN myostatin ELISA Kit; Myostatin ELISA Kit; Myostatin Propeptide ELISA Kit |
| Abbreviation | MSTN |
| Sample Types | serum, plasma, tissue homogenates |
| Detection Range | 0.625 ng/mL-20 ng/mL |
| Assay Time | 1-5h |
| Sample Loading Volume | 50-100ul |
| Detection Wavelength | 450 nm |
| Research Area | Signal Transduction |
| Assay Principle | quantitative |
| Measurement | Sandwich |
| Precision | headers: Intra-assay Precision (Precision within an assay): CV%<15%; rows: Intra-assay Precision (Precision within an assay): CV%<15%: Intra-assay Precision (Precision within an assay): CV%<15%; 1: Three samples of known concentration were tested twenty times on one plate to assess.; Intra-assay Precision (Precision within an assay): CV%<15%: Inter-assay Precision (Precision between assays): CV%<15%; 1: Three samples of known concentration were tested in twenty assays to assess.; |
| Linearity | headers: To assess the linearity of the assay, samples were spiked with high concentrations of human MSTN in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.; rows: To assess the linearity of the assay, samples were spiked with high concentrations of human MSTN in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.: To assess the linearity of the assay, samples were spiked with high concentrations of human MSTN in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.; 2: Sample; 3: Serum(n=4); 1: 1:1; 2: Average %; 3: 95; 1: Range %; 2: 92-99; 1: 1:2; 2: Average %; 3: 94; 1: Range %; 2: 91-98; 1: 1:4; 2: Average %; 3: 101; 1: Range %; 2: 97-105; 1: 1:8; 2: Average %; 3: 86; 1: Range %; 2: 82-90 |
| Recovery | headers: The recovery of human MSTN spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.; rows: The recovery of human MSTN spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.: The recovery of human MSTN spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.; 1: Sample Type; 2: Average % Recovery; 3: Range; 1: Serum (n=5); 2: 93; 3: 87-97; 1: EDTA plasma (n=4); 2: 86; 3: 82-91; ; |
| Typical Data | headers: These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.; rows: These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.: These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.; 1: ng/ml OD1 OD2 Average Corrected 20 1.851 1.992 1.922 1.878 10 1.058 1.136 1.097 1.053 4.375 0.502 0.524 0.513 0.469 1.875 0.268 0.255 0.262 0.218 0.625 0.116 0.119 0.118 0.074 0 0.043 0.045 0.044; 2: ng/ml; 3: OD1; 4: OD2; 5: Average; 6: Corrected; 8: 20; 9: 1.851; 10: 1.992; 11: 1.922; 12: 1.878; 14: 10; 15: 1.058; 16: 1.136; 17: 1.097; 18: 1.053; 20: 4.375; 21: 0.502; 22: 0.524; 23: 0.513; 24: 0.469; 26: 1.875; 27: 0.268; 28: 0.255; 29: 0.262; 30: 0.218; 32: 0.625; 33: 0.116; 34: 0.119; 35: 0.118; 36: 0.074; 38: 0; 39: 0.043; 40: 0.045; 41: 0.044; 1: ng/ml; 2: OD1; 3: OD2; 4: Average; 5: Corrected; 1: 20; 2: 1.851; 3: 1.992; 4: 1.922; 5: 1.878; 1: 10; 2: 1.058; 3: 1.136; 4: 1.097; 5: 1.053; 1: 4.375; 2: 0.502; 3: 0.524; 4: 0.513; 5: 0.469; 1: 1.875; 2: 0.268; 3: 0.255; 4: 0.262; 5: 0.218; 1: 0.625; 2: 0.116; 3: 0.119; 4: 0.118; 5: 0.074; 1: 0; 2: 0.043; 3: 0.045; 4: 0.044 |
| Materials provided | A micro ELISA plate ---The 96-well plate has been pre-coated with an anti-human MSTN antibody. Six vials standard (5 ml/bottle) ---Dilute a bottle of the standard at dilution series, read the OD values, and then draw a standard curve. One vial HRP-conjugated MSTN antibody (6 ml/bottle) ---Bind to the MSTN in the samples or standards and react with the substrate to make the solution chromogenic. Substrate A (1 x 7 ml) Substrate B (1 x 7 ml) One vial Stop Solution (7ml/bottle) ---Stop the color reaction. The solution color immediately turns from blue to yellow. Four Adhesive Strips (For 96 wells) ---Cover the microplate when incubation. An instruction manual |
| Materials not provided | A microplate reader capable of measuring absorbance at 450 nm, with the correction wavelength set at 600 nm or 630 nm. An incubator can provide stable incubation conditions up to 37°C±5°C. Centrifuge Vortex Squirt bottle, manifold dispenser, or automated microplate washer Absorbent paper for blotting the microtiter plate 50-300ul multi-channel micropipette Pipette tips Single-channel micropipette with different ranges 100ml and 500ml graduated cylinders Deionized or distilled water Timer Test tubes for dilution |
| ELISA Data Analysis | ELISA Standard Curve & Curve Expert software |
| Shelf Life | 6 months |
| Description | This Human MSTN ELISA Kit was designed for the quantitative measurement of Human MSTN protein in serum, plasma, tissue homogenates. It is a Sandwich ELISA kit, its detection range is 0.625 ng/mL-20 ng/mL and the sensitivity is 0.312 ng/mL. |
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