Human NADPH oxidase 4(NOX4) ELISA kit CSB-EL015961HU CUSABIO
$50.00
In stock
SKU
CSB-EL015961HU
Product Details
| Target Name | NADPH oxidase 4 |
| Uniprot No. | Q9NPH5 |
| Species | Homo sapiens (Human) |
| Storage | Store at 2-8°C. Please refer to protocol. |
| Sensitivity | 2.34 pg/mL |
| Troubleshooting and FAQs | ELISA kit FAQs |
| Lead Time | 3-5 working days after you place the order, and it takes another 3-5 days for delivery via DHL or FedEx. |
| Associated names | NOX4; RENOX; NADPH oxidase 4; Kidney oxidase-1; KOX-1; Kidney superoxide-producing NADPH oxidase; Renal NAD(PH-oxidase |
| Abbreviation | NOX4 |
| Sample Types | serum, plasma, tissue homogenates, cell lysates |
| Detection Range | 9.38 pg/mL-600 pg/mL |
| Assay Time | 1-5h |
| Sample Loading Volume | 50-100ul |
| Detection Wavelength | 450 nm |
| Research Area | Cardiovascular |
| Assay Principle | quantitative |
| Measurement | Sandwich |
| Precision | headers: Intra-assay Precision (Precision within an assay): CV%<8%; rows: Intra-assay Precision (Precision within an assay): CV%<8%: Intra-assay Precision (Precision within an assay): CV%<8%; 1: Three samples of known concentration were tested twenty times on one plate to assess.; Intra-assay Precision (Precision within an assay): CV%<8%: Inter-assay Precision (Precision between assays): CV%<10%; 1: Three samples of known concentration were tested in twenty assays to assess.; |
| Linearity | headers: To assess the linearity of the assay, samples were spiked with high concentrations of human NOX4 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.; rows: To assess the linearity of the assay, samples were spiked with high concentrations of human NOX4 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.: To assess the linearity of the assay, samples were spiked with high concentrations of human NOX4 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.; 2: Sample; 3: Serum(n=4); 1: 1:5; 2: Average %; 3: 84; 1: Range %; 2: 80-88; 1: 1:10; 2: Average %; 3: 99; 1: Range %; 2: 95-103; 1: 1:20; 2: Average %; 3: 88; 1: Range %; 2: 84-92; 1: 1:40; 2: Average %; 3: 107; 1: Range %; 2: 100-115 |
| Recovery | headers: The recovery of human NOX4 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.; rows: The recovery of human NOX4 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.: The recovery of human NOX4 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.; 1: Sample Type; 2: Average % Recovery; 3: Range; 1: Serum (n=5); 2: 94; 3: 90-98; 1: EDTA plasma (n=4); 2: 90; 3: 85-94; ; |
| Typical Data | headers: These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.; rows: These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.: These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.; 1: pg/ml OD1 OD2 Average Corrected 600 2.660 2.652 2.656 2.490 300 2.194 2.075 2.135 1.969 150 1.401 1.414 1.408 1.242 75 0.802 0.789 0.796 0.630 37.5 0.469 0.486 0.478 0.312 18.75 0.315 0.329 0.322 0.156 9.38 0.208 0.216 0.212 0.046 0 0.162 0.169 0.166; 2: pg/ml; 3: OD1; 4: OD2; 5: Average; 6: Corrected; 8: 600; 9: 2.660; 10: 2.652; 11: 2.656; 12: 2.490; 14: 300; 15: 2.194; 16: 2.075; 17: 2.135; 18: 1.969; 20: 150; 21: 1.401; 22: 1.414; 23: 1.408; 24: 1.242; 26: 75; 27: 0.802; 28: 0.789; 29: 0.796; 30: 0.630; 32: 37.5; 33: 0.469; 34: 0.486; 35: 0.478; 36: 0.312; 38: 18.75; 39: 0.315; 40: 0.329; 41: 0.322; 42: 0.156; 44: 9.38; 45: 0.208; 46: 0.216; 47: 0.212; 48: 0.046; 50: 0; 51: 0.162; 52: 0.169; 53: 0.166; 1: pg/ml; 2: OD1; 3: OD2; 4: Average; 5: Corrected; 1: 600; 2: 2.660; 3: 2.652; 4: 2.656; 5: 2.490; 1: 300; 2: 2.194; 3: 2.075; 4: 2.135; 5: 1.969; 1: 150; 2: 1.401; 3: 1.414; 4: 1.408; 5: 1.242; 1: 75; 2: 0.802; 3: 0.789; 4: 0.796; 5: 0.630; 1: 37.5; 2: 0.469; 3: 0.486; 4: 0.478; 5: 0.312; 1: 18.75; 2: 0.315; 3: 0.329; 4: 0.322; 5: 0.156; 1: 9.38; 2: 0.208; 3: 0.216; 4: 0.212; 5: 0.046; 1: 0; 2: 0.162; 3: 0.169; 4: 0.166 |
| ELISA Data Analysis | ELISA Standard Curve & Curve Expert software |
| Shelf Life | 6 months |
| Description | This Human NOX4 ELISA Kit was designed for the quantitative measurement of Human NOX4 protein in serum, plasma, tissue homogenates, cell lysates. It is a Sandwich ELISA kit, its detection range is 9.38 pg/mL-600 pg/mL and the sensitivity is 2.34 pg/mL. |
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