Human Nesfatin-1 ELISA Kit CSB-E15050h CUSABIO
$50.00
In stock
SKU
CSB-E15050h
Product Details
| Target Name | Nesfatin-1 |
| Species | Homo sapiens (Human) |
| Storage | Store at 2-8°C. Please refer to protocol. |
| Sensitivity | 7.81 pg/mL |
| Troubleshooting and FAQs | ELISA kit FAQs |
| Lead Time | 3-5 working days after you place the order, and it takes another 3-5 days for delivery via DHL or FedEx. |
| Abbreviation | Nesfatin-1 |
| Sample Types | serum, plasma, tissue homogenates |
| Detection Range | 31.25 pg/mL-2000 pg/mL |
| Assay Time | 1-5h |
| Sample Loading Volume | 50-100ul |
| Detection Wavelength | 450 nm |
| Research Area | Others |
| Assay Principle | quantitative |
| Measurement | Sandwich |
| Precision | headers: Intra-assay Precision (Precision within an assay): CV%<8%; rows: Intra-assay Precision (Precision within an assay): CV%<8%: Intra-assay Precision (Precision within an assay): CV%<8%; 1: Three samples of known concentration were tested twenty times on one plate to assess.; Intra-assay Precision (Precision within an assay): CV%<8%: Inter-assay Precision (Precision between assays): CV%<10%; 1: Three samples of known concentration were tested in twenty assays to assess.; |
| Linearity | headers: To assess the linearity of the assay, samples were spiked with high concentrations of human Nesfatin-1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.; rows: To assess the linearity of the assay, samples were spiked with high concentrations of human Nesfatin-1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.: To assess the linearity of the assay, samples were spiked with high concentrations of human Nesfatin-1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.; 2: Sample; 3: Serum(n=4); 1: 1:5; 2: Average %; 3: 96; 1: Range %; 2: 80-105; 1: 1:10; 2: Average %; 3: 99; 1: Range %; 2: 91-111; 1: 1:20; 2: Average %; 3: 104; 1: Range %; 2: 92-112; 1: 1:40; 2: Average %; 3: 93; 1: Range %; 2: 86-100 |
| Recovery | headers: The recovery of human Nesfatin-1 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.; rows: The recovery of human Nesfatin-1 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.: The recovery of human Nesfatin-1 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.; 1: Sample Type; 2: Average % Recovery; 3: Range; 1: Serum (n=5); 2: 94; 3: 89-98; 1: EDTA plasma (n=4); 2: 97; 3: 90-107; ; |
| Typical Data | headers: These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.; rows: These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.: These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.; 1: pg/ml OD1 OD2 Average Corrected 2000 2.617 2.557 2.587 2.463 1000 2.205 2.356 2.281 2.157 500 1.636 1.767 1.702 1.578 250 1.043 1.058 1.051 0.927 125 0.609 0.642 0.626 0.502 62.5 0.352 0.346 0.349 0.225 31.25 0.226 0.243 0.235 0.111 0 0.122 0.125 0.124; 2: pg/ml; 3: OD1; 4: OD2; 5: Average; 6: Corrected; 8: 2000; 9: 2.617; 10: 2.557; 11: 2.587; 12: 2.463; 14: 1000; 15: 2.205; 16: 2.356; 17: 2.281; 18: 2.157; 20: 500; 21: 1.636; 22: 1.767; 23: 1.702; 24: 1.578; 26: 250; 27: 1.043; 28: 1.058; 29: 1.051; 30: 0.927; 32: 125; 33: 0.609; 34: 0.642; 35: 0.626; 36: 0.502; 38: 62.5; 39: 0.352; 40: 0.346; 41: 0.349; 42: 0.225; 44: 31.25; 45: 0.226; 46: 0.243; 47: 0.235; 48: 0.111; 50: 0; 51: 0.122; 52: 0.125; 53: 0.124; 1: pg/ml; 2: OD1; 3: OD2; 4: Average; 5: Corrected; 1: 2000; 2: 2.617; 3: 2.557; 4: 2.587; 5: 2.463; 1: 1000; 2: 2.205; 3: 2.356; 4: 2.281; 5: 2.157; 1: 500; 2: 1.636; 3: 1.767; 4: 1.702; 5: 1.578; 1: 250; 2: 1.043; 3: 1.058; 4: 1.051; 5: 0.927; 1: 125; 2: 0.609; 3: 0.642; 4: 0.626; 5: 0.502; 1: 62.5; 2: 0.352; 3: 0.346; 4: 0.349; 5: 0.225; 1: 31.25; 2: 0.226; 3: 0.243; 4: 0.235; 5: 0.111; 1: 0; 2: 0.122; 3: 0.125; 4: 0.124 |
| ELISA Data Analysis | ELISA Standard Curve & Curve Expert software |
| Shelf Life | 6 months |
| Description | This human nesfatin-1 ELISA kit employs the quantitative sandwich enzyme immunoassay technique to measure the levels of human nesfatin-1 in different samples, including serum, plasma, or tissue homogenates. The enzyme-substrate chromogenic reaction is also used to amplify the signal and quantify the levels of the analyte through the intensity of the colored product. The color intensity positively correlates with the amount of nesfatin-1 bound in the initial step. Nesfatin-1 is a potent anorexigenic peptide that is processed from the precursor-peptide NEFA/nucleobindin 2 (NUCB2) and is involved in the modulation of homeostatic feeding. In the hypothalamus and brainstem, nesfatin-1 recruits the oxytocin, the melancortin, and other systems to relay its anorexigenic effects. In addition to anorexigenic properties, nesfatin-1 has also been found to perform other important functions, especially associated with energy homeostasis. such as energy expenditure and glucose homeostasis. Nesfatin-1 also has pleiotropic effects on the level of cardiovascular and digestive systems, as well as being implicated in stress response, sleep, and reproduction. |
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