Human Neurogenic locus notch homolog protein 1(NOTCH1) ELISA kit CSB-EL015949HU CUSABIO
$50.00
In stock
SKU
CSB-EL015949HU
Product Details
| Target Name | Notch homolog 1, translocation-associated (Drosophila) |
| Uniprot No. | P46531 |
| Species | Homo sapiens (Human) |
| Storage | Store at 2-8°C. Please refer to protocol. |
| Sensitivity | 19.5 pg/mL |
| Troubleshooting and FAQs | ELISA kit FAQs |
| Lead Time | 3-5 working days after you place the order, and it takes another 3-5 days for delivery via DHL or FedEx. |
| Associated names | hN1 ELISA Kit; Neurogenic locus Notch homolog protein 1 ELISA Kit; NICD ELISA Kit; NOTC1_HUMAN ELISA Kit; Notch 1 ELISA Kit; Notch 1 intracellular domain ELISA Kit; Notch homolog 1 translocation associated ELISA Kit; notch1 ELISA Kit; TAN1 ELISA Kit; Translocation associated Notch protein TAN1 ELISA Kit; Translocation-associated notch protein TAN-1 ELISA Kit |
| Abbreviation | NOTCH1 |
| Sample Types | serum, plasma, tissue homogenates |
| Detection Range | 78 pg/mL-5000 pg/mL |
| Assay Time | 1-5h |
| Sample Loading Volume | 50-100ul |
| Detection Wavelength | 450 nm |
| Research Area | Epigenetics and Nuclear Signaling |
| Assay Principle | quantitative |
| Measurement | Sandwich |
| Precision | headers: Intra-assay Precision (Precision within an assay): CV%<8%; rows: Intra-assay Precision (Precision within an assay): CV%<8%: Intra-assay Precision (Precision within an assay): CV%<8%; 1: Three samples of known concentration were tested twenty times on one plate to assess.; Intra-assay Precision (Precision within an assay): CV%<8%: Inter-assay Precision (Precision between assays): CV%<10%; 1: Three samples of known concentration were tested in twenty assays to assess.; 1: Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 20 20 20 20 20 20 Mean(pg/ml) 631.041 602.691 606.472 587.560 598.909 629.151 SD 0.025 0.021 0.026 0.029 0.033 0.031 CV(%) 5.144 4.459 5.497 6.263 7.036 6.392; 3: Intra-Assay Precision; 4: Inter-Assay Precision; 5: Sample; 6: 1; 7: 2; 8: 3; 9: 1; 10: 2; 11: 3; 12: n; 13: 20; 14: 20; 15: 20; 16: 20; 17: 20; 18: 20; 19: Mean(pg/ml); 20: 631.041; 21: 602.691; 22: 606.472; 23: 587.560; 24: 598.909; 25: 629.151; 26: SD; 27: 0.025; 28: 0.021; 29: 0.026; 30: 0.029; 31: 0.033; 32: 0.031; 33: CV(%); 34: 5.144; 35: 4.459; 36: 5.497; 37: 6.263; 38: 7.036; 39: 6.392; 2: Intra-Assay Precision; 3: Inter-Assay Precision; 1: Sample; 2: 1; 3: 2; 4: 3; 5: 1; 6: 2; 7: 3; 1: n; 2: 20; 3: 20; 4: 20; 5: 20; 6: 20; 7: 20; 1: Mean(pg/ml); 2: 631.041; 3: 602.691; 4: 606.472; 5: 587.560; 6: 598.909; 7: 629.151; 1: SD; 2: 0.025; 3: 0.021; 4: 0.026; 5: 0.029; 6: 0.033; 7: 0.031; 1: CV(%); 2: 5.144; 3: 4.459; 4: 5.497; 5: 6.263; 6: 7.036; 7: 6.392; |
| Linearity | headers: To assess the linearity of the assay, samples were spiked with high concentrations of Human NOTCH1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.; rows: To assess the linearity of the assay, samples were spiked with high concentrations of Human NOTCH1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.: To assess the linearity of the assay, samples were spiked with high concentrations of Human NOTCH1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.; 2: Sample; 3: Serum(n=4); 1: 1:2; 2: Average %; 3: 97; 1: Range %; 2: 94-101; 1: 1:4; 2: Average %; 3: 95; 1: Range %; 2: 89-99; 1: 1:8; 2: Average %; 3: 93; 1: Range %; 2: 89-97; 1: 1:16; 2: Average %; 3: 86; 1: Range %; 2: 82-92; |
| Recovery | headers: The recovery of Human NOTCH1 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.; rows: The recovery of Human NOTCH1 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.: The recovery of Human NOTCH1 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.; 1: Sample Type; 2: Average % Recovery; 3: Range%; 1: Serum (n=5); 2: 96; 3: 88-102; 1: EDTA plasma (n=4); 2: 89; 3: 83-94; |
| Typical Data | headers: These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.; rows: These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.: These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.; ; ; 1: pg/ml; 2: OD1; 3: OD2; 4: Average; 5: Corrected; 1: 5000; 2: 2.426; 3: 2.614; 4: 2.520; 5: 2.375; 1: 2500; 2: 1.508; 3: 1.423; 4: 1.466; 5: 1.321; 1: 1250; 2: 0.784; 3: 0.825; 4: 0.805; 5: 0.660; 1: 625; 2: 0.496; 3: 0.477; 4: 0.487; 5: 0.342; 1: 312; 2: 0.309; 3: 0.317; 4: 0.313; 5: 0.168; 1: 156; 2: 0.255; 3: 0.264; 4: 0.260; 5: 0.115; 1: 78; 2: 0.197; 3: 0.192; 4: 0.195; 5: 0.050; 1: 0; 2: 0.144; 3: 0.145; 4: 0.145; |
| ELISA Data Analysis | ELISA Standard Curve & Curve Expert software |
| Shelf Life | 6 months |
| Description | The human neurogenic locus notch homolog protein 1 (NOTCH1) ELISA kit is suitable for quantitatively determining human NOTCH1 in serum, plasma, or tissue homogenates. This assay employs the bi-antibody sandwich technique and enzyme-substrate chromogenic reaction to quantify human NOTCH1 levels in the sample. The amount of synthesized colored product is positively related to the analyte of interest in the sample. NOTCH1 plays an essential role in angiogenic vascular remodeling and embryonic development. Loss of either NOTCH1 or components of the Notch signaling pathway leads to early embryonic demise related to defects in vasculogenesis, somitogenesis, and cardiogenesis. Recent studies have shown that NOTCH1 is involved in numerous human cancers, including breast cancer, leukemias, brain tumors, and many others. NOTCH1 promotes cell growth, survival, apoptosis, migration, and invasion of tumor cells which are essential for cancer development and progression. |
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