SARS-CoV-2 Neutralizing Antibody ELISA Kit CSB-EL33243HU CUSABIO
$50.00
In stock
SKU
CSB-EL33243HU
Product Details
| Species | Homo sapiens (Human) |
| Storage | Store at 2-8°C. Please refer to protocol. |
| Troubleshooting and FAQs | ELISA kit FAQs |
| Lead Time | 3-5 working days after you place the order, and it takes another 3-5 days for delivery via DHL or FedEx. |
| Associated names | 2019 Novel Coronavirus; Coronavirus; CoV; COVID-19 virus; HCoV-2; Human Coronavirus 2019; SARS2; SARS-CoV-2; Severe acute respiratory syndrome coronavirus 2 |
| Abbreviation | SARS-CoV-2 neutralizing Ab |
| Sample Types | serum, plasma |
| Assay Time | 1-5h |
| Sample Loading Volume | 50-100ul |
| Detection Wavelength | 450 nm |
| Research Area | Infectious Diseases |
| Assay Principle | qualitative |
| Measurement | Competitive |
| Precision | headers: Intra-assay Precision (Precision within an assay): CV%< 15 %; rows: Intra-assay Precision (Precision within an assay): CV%< 15 %: Intra-assay Precision (Precision within an assay): CV%< 15 %; Intra-assay Precision (Precision within an assay): CV%< 15 %: Three samples of known concentration were tested twenty times on one plate to assess.; Intra-assay Precision (Precision within an assay): CV%< 15 %: Inter-assay Precision (Precision between assays): CV%<1 5 %; Intra-assay Precision (Precision within an assay): CV%< 15 %: Three samples of known concentration were tested in twenty assays to assess. |
| Typical Data | headers: These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.; rows: These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.: These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.; |
| Materials provided | One 96-well Microtiter plate ( coated Microwells) -- This microwell plate has been pre-coated with recombinant SARS-CoV-2 S RBD antigen and can be dismountable into 12 strips of 8 wells. A bottle of Standard (10 x concentrate; 200 μl/vial) -- Dilute the standard at dilution series, read the OD values, and then draw a standard curve. A bottle of HRP-conjugated ACE2 (100 x concentrate; 60 μl/vial) -- ACE2 interacts with SARS-CoV-2 S RBD, mimicking the process of SARS-CoV-2 entry into the host cells. HRP catalyzes the TMB Substrate solution, eliciting a chromogenic reaction. A bottle of HRP-conjugate Diluent (10 ml/vial) -- Dilute the HRP-conjugated ACE2 solution. Two bottles of Sample Diluent (20 ml/vial) -- Reconstitute the standard and dilute the sample to an appropriate concentration. A bottle of Wash Buffer (25 x concentrate; 20 ml/vial) -- Wash away unbound or free substances. A bottle of TMB Substrate (10 ml/vial) -- Act as the chromogenic agent. TMB interacts with HRP, eliciting the solution turns blue. A bottle of Stop Solution (10 ml/vial) -- Stop the color reaction. The solution color immediately turns from blue to yellow. Four Adhesive Strips (For 96 wells) -- Seal the plate when incubation. One Instruction manual |
| Materials not provided | A microplate reader capable of measuring absorbance at 450 nm, with the correction wavelength set at 540 nm or 570 nm. An incubator that can provide stable incubation conditions up to 37°C±5°C. Centrifuge Vortex Squirt bottle, manifold dispenser, or automated microplate washer. Absorbent paper for blotting the microtiter plate. 50-300ul multi-channel micropipette 100ml and 500ml graduated cylinders. Deionized or distilled water. Pipette tips Timer Test tubes for dilution. |
| Shelf Life | 6 months |
| Description | CUSABIO's SARS-CoV-2 Neutralizing Antibody ELISA Kit is intended for qualitative measurement of neutralizing antibodies in human serum or plasma. SARS-CoV-2 neutralizing antibodies directly target the SARS-CoV-2 S receptor-binding domain (RBD), blocking the protein's interaction with the host ACE2 receptor, thus interfering with the viral attachment to the cell as well as its entry into the cell. When using the reference neutralizing antibody provided in the kit, the assay has a detection range of 9.75-10000 ng/ml, a sensitivity of as low as 9.75 ng/ml, and Intra- /Inter-assay Precision low than 15%. This detection kit is simple in operation and scalable in sample size (high throughput). Multiple validations display it has excellent sensitivity and high specificity. It is conducive to identify individuals with an adaptive immune response to SARS-CoV-2, indicating recent or prior infection. And it should not be used for the diagnosis of acute SARS-CoV-2 infection. |
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