Human SARS-CoV-2 S1 RBD IgG Antibody ELISA Kit CSB-EL33241HU CUSABIO
$50.00
In stock
SKU
CSB-EL33241HU
Product Details
| Uniprot No. | P0DTC2 |
| Species | Homo sapiens (Human) |
| Storage | Store at 2-8°C. Please refer to protocol. |
| Troubleshooting and FAQs | ELISA kit FAQs |
| Lead Time | 3-5 working days after you place the order, and it takes another 3-5 days for delivery via DHL or FedEx. |
| Associated names | S; 2; Spike glycoprotein; S glycoprotein; E2; Peplomer protein) |
| Abbreviation | SARS-CoV-2 S RBD Ab (IgG) |
| Sample Types | serum, plasma |
| Assay Time | 1-5h |
| Sample Loading Volume | 50-100ul |
| Detection Wavelength | 450 nm |
| Research Area | Infectious Diseases |
| Assay Principle | qualitative |
| Measurement | Indirect |
| Typical Data | headers: ; rows: ; ; 1: Test parameter; 2: specification; 3: test result; 1: Positive control; 2: >0.8; 3: 1.256; 1: Negative control; 2: <0.2; 3: 0.163; 1: Positive rate; 2: 20，Positive; 3: 100%; 1: Negative rate; 2: 20，Negative; 3: 100% |
| Materials provided | A 96-well Coated assay plate 1 -- This microplate has been pre-coated with human SARS-CoV-2 S1 RBD antigen. Negative Control (1 x 800 μl) -- It is free of the SARS-CoV-2 S1 RBD IgG antibody and used to preclude the false positive. Positive Control (1 x 800 μl) -- Used to evaluate the validity, stability, and comparability of the test results. HRP-conjugated anti-Human IgG antibody (100 x concentrate) (1 x 120 μl) -- Act as the detection antibody. HRP-conjugate Diluent (1 x 20 ml) -- Dilute the HRPconjugated anti-Human IgG antibody. Sample Diluent (2 x 20 ml) -- Dilute the sample solution. Wash Buffer (25 x concentrate) (1 x 20 ml) -- Wash the unbound regeat. TMB Substrate (1 x 10 ml) -- React with HRP, eliciting a chromogenic color reaction. Stop Solution (1 x 10 ml) -- Stop the color reaction. The solution turns from blue to yellow. Four Adhesive Strips (For 96 wells) -- Seal the microplate when incubation. An Instruction manual |
| Materials not provided | A microplate reader capable of measuring absorbance at 450 nm, with the correction wavelength set at 540 nm or 570 nm. An incubator that can provide stable incubation conditions up to 37°C±5°C. Centrifuge Vortex Squirt bottle, manifold dispenser, or automated microplate washer. Absorbent paper for blotting the microtiter plate. 50-300ul multi-channel micropipette 100ml and 500ml graduated cylinders. Deionized or distilled water. Pipette tips Timer Test tubes for dilution. |
| Shelf Life | 6 months |
| Description | The product CSB-EL33241HU is an easy-to-use ELISA Kit intended for the qualitative detection of SARS-CoV-2 S1 RBD IgG antibody in human serum and plasma in vitro. SARS-CoV-2 S1 RBD IgG is an indicator of a recent or prior COVID infection. Infected patients develop IgG against SARS-CoV-2 S1 RBD 7 days post-infection, and IgG content peaks in the third week (IgM was close to vanishing at this time). IgG lasts in the blood for months or even years. After secondary infection, IgG levels in the body of patients increase rapidly and substantially in the short term and remain in the body for a long time, while IgM rarely increases. Serological testing may help to diagnose suspected patients with negative RT–PCR (real-time polymerase chain reaction) results and identify asymptomatic infections. |
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