Human Pro Atrial Natriuretic Peptide,Pro-ANP ELISA Kit CSB-E05149h CUSABIO
$50.00
In stock
SKU
CSB-E05149h
Product Details
| Target Name | Pro Atrial Natriuretic Peptide,Pro-ANP |
| Species | Homo sapiens (Human) |
| Storage | Store at 2-8°C. Please refer to protocol. |
| Sensitivity | 15.6 pg/mL |
| Troubleshooting and FAQs | ELISA kit FAQs |
| Lead Time | 3-5 working days after you place the order, and it takes another 3-5 days for delivery via DHL or FedEx. |
| Abbreviation | Pro-ANP |
| Sample Types | serum, plasma, tissue homogenates |
| Detection Range | 62.5 pg/mL-4000 pg/mL |
| Assay Time | 1-5h |
| Sample Loading Volume | 50-100ul |
| Detection Wavelength | 450 nm |
| Research Area | Cardiovascular |
| Assay Principle | quantitative |
| Measurement | Sandwich |
| Precision | headers: Intra-assay Precision (Precision within an assay): CV%<8%; rows: Intra-assay Precision (Precision within an assay): CV%<8%: Intra-assay Precision (Precision within an assay): CV%<8%; 1: Three samples of known concentration were tested twenty times on one plate to assess.; Intra-assay Precision (Precision within an assay): CV%<8%: Inter-assay Precision (Precision between assays): CV%<10%; 1: Three samples of known concentration were tested in twenty assays to assess.; |
| Linearity | headers: To assess the linearity of the assay, samples were spiked with high concentrations of human Pro-ANP in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.; rows: To assess the linearity of the assay, samples were spiked with high concentrations of human Pro-ANP in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.: To assess the linearity of the assay, samples were spiked with high concentrations of human Pro-ANP in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.; 2: Sample; 3: Serum(n=4); 1: 1:1; 2: Average %; 3: 93; 1: Range %; 2: 86-97; 1: 1:2; 2: Average %; 3: 85; 1: Range %; 2: 80-89; 1: 1:4; 2: Average %; 3: 92; 1: Range %; 2: 87-98; 1: 1:8; 2: Average %; 3: 104; 1: Range %; 2: 96-108 |
| Recovery | headers: The recovery of human Pro-ANP spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.; rows: The recovery of human Pro-ANP spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.: The recovery of human Pro-ANP spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.; ; 1: Sample Type; 2: Average % Recovery; 3: Range; 1: Serum (n=5); 2: 89; 3: 84-95; 1: EDTA plasma (n=4); 2: 87; 3: 82-93; ; |
| Typical Data | headers: These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.; rows: These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.: These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.; ; 1: pg/ml OD1 OD2 Average Corrected 4000 1.943 1.896 1.920 1.766 2000 1.452 1.366 1.409 1.255 1000 0.998 0.968 0.983 0.829 500 0.646 0.657 0.652 0.498 250 0.467 0.454 0.461 0.307 125 0.322 0.306 0.314 0.160 62.5 0.209 0.203 0.206 0.052 0 0.155 0.153 0.154; 2: pg/ml; 3: OD1; 4: OD2; 5: Average; 6: Corrected; 9: 4000; 10: 1.943; 11: 1.896; 12: 1.920; 13: 1.766; 16: 2000; 17: 1.452; 18: 1.366; 19: 1.409; 20: 1.255; 23: 1000; 24: 0.998; 25: 0.968; 26: 0.983; 27: 0.829; 30: 500; 31: 0.646; 32: 0.657; 33: 0.652; 34: 0.498; 37: 250; 38: 0.467; 39: 0.454; 40: 0.461; 41: 0.307; 44: 125; 45: 0.322; 46: 0.306; 47: 0.314; 48: 0.160; 51: 62.5; 52: 0.209; 53: 0.203; 54: 0.206; 55: 0.052; 58: 0; 59: 0.155; 60: 0.153; 61: 0.154; 1: pg/ml; 2: OD1; 3: OD2; 4: Average; 5: Corrected; 1: 4000; 2: 1.943; 3: 1.896; 4: 1.920; 5: 1.766; 1: 2000; 2: 1.452; 3: 1.366; 4: 1.409; 5: 1.255; 1: 1000; 2: 0.998; 3: 0.968; 4: 0.983; 5: 0.829; 1: 500; 2: 0.646; 3: 0.657; 4: 0.652; 5: 0.498; 1: 250; 2: 0.467; 3: 0.454; 4: 0.461; 5: 0.307; 1: 125; 2: 0.322; 3: 0.306; 4: 0.314; 5: 0.160; 1: 62.5; 2: 0.209; 3: 0.203; 4: 0.206; 5: 0.052; 1: 0; 2: 0.155; 3: 0.153; 4: 0.154 |
| ELISA Data Analysis | ELISA Standard Curve & Curve Expert software |
| Shelf Life | 6 months |
| Description | This Human Pro-ANP ELISA Kit was designed for the quantitative measurement of Human Pro-ANP protein in serum, plasma, tissue homogenates. It is a Sandwich ELISA kit, its detection range is 62.5 pg/mL-4000 pg/mL and the sensitivity is 15.6 pg/mL. |
MDSD
Reviews
Write Your Own Review