Bovine Fibronectin,FN ELISA Kit CSB-E13781B CUSABIO
$50.00
In stock
SKU
CSB-E13781B
Product Details
| Target Name | fibronectin 1 |
| Uniprot No. | P07589 |
| Species | Bos taurus (Bovine) |
| Storage | Store at 2-8°C. Please refer to protocol. |
| Sensitivity | 0.137 μg/mL |
| Troubleshooting and FAQs | ELISA kit FAQs |
| Lead Time | 3-5 working days after you place the order, and it takes another 3-5 days for delivery via DHL or FedEx. |
| Associated names | FN1Fibronectin ELISA Kit; FN) [Cleaved into: Anastellin] ELISA Kit |
| Abbreviation | FN1 |
| Sample Types | serum, plasma, cell culture supernates, tissue homogenates, cell lysates |
| Detection Range | 0.137 μg/mL-100 μg/mL |
| Assay Time | 1-5h |
| Sample Loading Volume | 50-100ul |
| Detection Wavelength | 450 nm |
| Research Area | Immunology |
| Assay Principle | quantitative |
| Measurement | Sandwich |
| Precision | headers: Intra-assay Precision (Precision within an assay): CV%<8%; rows: Intra-assay Precision (Precision within an assay): CV%<8%: Intra-assay Precision (Precision within an assay): CV%<8%; 1: Three samples of known concentration were tested twenty times on one plate to assess.; Intra-assay Precision (Precision within an assay): CV%<8%: Inter-assay Precision (Precision between assays): CV%<10%; 1: Three samples of known concentration were tested in twenty assays to assess.; |
| Linearity | headers: To assess the linearity of the assay, samples were spiked with high concentrations of bovine FN in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.; rows: To assess the linearity of the assay, samples were spiked with high concentrations of bovine FN in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.: To assess the linearity of the assay, samples were spiked with high concentrations of bovine FN in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.; 2: Sample; 3: Serum(n=4); 1: 1:5; 2: Average %; 3: 92; 1: Range %; 2: 85-102; 1: 1:10; 2: Average %; 3: 97; 1: Range %; 2: 91-110; 1: 1:20; 2: Average %; 3: 89; 1: Range %; 2: 83-96; 1: 1:40; 2: Average %; 3: 95; 1: Range %; 2: 84-100 |
| Recovery | headers: The recovery of bovine FN spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.; rows: The recovery of bovine FN spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.: The recovery of bovine FN spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.; ; 1: Sample Type; 2: Average % Recovery; 3: Range; 1: Serum (n=5); 2: 96; 3: 88-105; 1: EDTA plasma (n=4); 2: 97; 3: 92-105; ; |
| Typical Data | headers: These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.; rows: These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.: These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.; ; 1: µg/ml OD1 OD2 Average Corrected 100 1.839 1.803 1.821 1.677 33 1.657 1.649 1.653 1.509 11 1.451 1.435 1.443 1.299 3.7 0.958 0.992 0.975 0.831 1.2 0.643 0.667 0.655 0.511 0.4 0.372 0.354 0.363 0.219 0.13 0.251 0.243 0.247 0.103 0 0.145 0.142 0.144; 2: µg/ml; 3: OD1; 4: OD2; 5: Average; 6: Corrected; 9: 100; 10: 1.839; 11: 1.803; 12: 1.821; 13: 1.677; 16: 33; 17: 1.657; 18: 1.649; 19: 1.653; 20: 1.509; 23: 11; 24: 1.451; 25: 1.435; 26: 1.443; 27: 1.299; 30: 3.7; 31: 0.958; 32: 0.992; 33: 0.975; 34: 0.831; 37: 1.2; 38: 0.643; 39: 0.667; 40: 0.655; 41: 0.511; 44: 0.4; 45: 0.372; 46: 0.354; 47: 0.363; 48: 0.219; 51: 0.13; 52: 0.251; 53: 0.243; 54: 0.247; 55: 0.103; 58: 0; 59: 0.145; 60: 0.142; 61: 0.144; 1: µg/ml; 2: OD1; 3: OD2; 4: Average; 5: Corrected; 1: 100; 2: 1.839; 3: 1.803; 4: 1.821; 5: 1.677; 1: 33; 2: 1.657; 3: 1.649; 4: 1.653; 5: 1.509; 1: 11; 2: 1.451; 3: 1.435; 4: 1.443; 5: 1.299; 1: 3.7; 2: 0.958; 3: 0.992; 4: 0.975; 5: 0.831; 1: 1.2; 2: 0.643; 3: 0.667; 4: 0.655; 5: 0.511; 1: 0.4; 2: 0.372; 3: 0.354; 4: 0.363; 5: 0.219; 1: 0.13; 2: 0.251; 3: 0.243; 4: 0.247; 5: 0.103; 1: 0; 2: 0.145; 3: 0.142; 4: 0.144 |
| ELISA Data Analysis | ELISA Standard Curve & Curve Expert software |
| Shelf Life | 6 months |
| Description | This Bovine FN1 ELISA Kit was designed for the quantitative measurement of Bovine FN1 protein in serum, plasma, cell culture supernates, tissue homogenates, cell lysates. It is a Sandwich ELISA kit, its detection range is 0.137 μg/mL-100 μg/mL and the sensitivity is 0.137 μg/mL. |
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