Bovine Insulin-like growth factor II(IGF2) ELISA kit CSB-EL011088BO CUSABIO
$50.00
In stock
SKU
CSB-EL011088BO
Product Details
| Target Name | insulin-like growth factor 2 (somatomedin A) |
| Uniprot No. | P07456 |
| Species | Bos taurus (Bovine) |
| Storage | Store at 2-8°C. Please refer to protocol. |
| Sensitivity | 7.81 ng/mL |
| Troubleshooting and FAQs | ELISA kit FAQs |
| Lead Time | 3-5 working days after you place the order, and it takes another 3-5 days for delivery via DHL or FedEx. |
| Associated names | IGF2Insulin-like growth factor II ELISA Kit; IGF-II ELISA Kit; Erythrotropin) [Cleaved into: Insulin-like growth factor II; Preptin] ELISA Kit |
| Abbreviation | IGF2 |
| Sample Types | serum, plasma |
| Detection Range | 31.25 ng/mL-2000 ng/mL |
| Assay Time | 1-5h |
| Sample Loading Volume | 50-100ul |
| Detection Wavelength | 450 nm |
| Research Area | Metabolism |
| Assay Principle | quantitative |
| Measurement | Sandwich |
| Precision | headers: Intra-assay Precision (Precision within an assay): CV%<8%; rows: Intra-assay Precision (Precision within an assay): CV%<8%: Intra-assay Precision (Precision within an assay): CV%<8%; 1: Three samples of known concentration were tested twenty times on one plate to assess.; Intra-assay Precision (Precision within an assay): CV%<8%: Inter-assay Precision (Precision between assays): CV%<10%; 1: Three samples of known concentration were tested in twenty assays to assess.; |
| Linearity | headers: To assess the linearity of the assay, samples were spiked with high concentrations of bovine IGF2 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.; rows: To assess the linearity of the assay, samples were spiked with high concentrations of bovine IGF2 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.: To assess the linearity of the assay, samples were spiked with high concentrations of bovine IGF2 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.; 2: Sample; 3: Serum(n=4); 1: 1:1; 2: Average %; 3: 107; 1: Range %; 2: 100-114; 1: 1:2; 2: Average %; 3: 103; 1: Range %; 2: 101-106; 1: 1:4; 2: Average %; 3: 87; 1: Range %; 2: 84-91; 1: 1:8; 2: Average %; 3: 94; 1: Range %; 2: 90-96 |
| Recovery | headers: The recovery of bovine IGF2 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.; rows: The recovery of bovine IGF2 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.: The recovery of bovine IGF2 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.; ; 1: Sample Type; 2: Average % Recovery; 3: Range; 1: Serum (n=5); 2: 95; 3: 90-100; 1: EDTA plasma (n=4); 2: 88; 3: 85-91; ; |
| Typical Data | headers: These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.; rows: These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.: These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.; ; 1: ng/ml OD1 OD2 Average Corrected 2000 1.958 1.952 1.955 1.858 1000 1.166 1.076 1.121 1.024 500 0.671 0.654 0.663 0.566 250 0.398 0.389 0.394 0.297 125 0.263 0.254 0.259 0.162 62.5 0.177 0.169 0.173 0.076 31.25 0.135 0.129 0.132 0.035 0 0.096 0.097 0.097; 2: ng/ml; 3: OD1; 4: OD2; 5: Average; 6: Corrected; 9: 2000; 10: 1.958; 11: 1.952; 12: 1.955; 13: 1.858; 16: 1000; 17: 1.166; 18: 1.076; 19: 1.121; 20: 1.024; 23: 500; 24: 0.671; 25: 0.654; 26: 0.663; 27: 0.566; 30: 250; 31: 0.398; 32: 0.389; 33: 0.394; 34: 0.297; 37: 125; 38: 0.263; 39: 0.254; 40: 0.259; 41: 0.162; 44: 62.5; 45: 0.177; 46: 0.169; 47: 0.173; 48: 0.076; 51: 31.25; 52: 0.135; 53: 0.129; 54: 0.132; 55: 0.035; 58: 0; 59: 0.096; 60: 0.097; 61: 0.097; 1: ng/ml; 2: OD1; 3: OD2; 4: Average; 5: Corrected; 1: 2000; 2: 1.958; 3: 1.952; 4: 1.955; 5: 1.858; 1: 1000; 2: 1.166; 3: 1.076; 4: 1.121; 5: 1.024; 1: 500; 2: 0.671; 3: 0.654; 4: 0.663; 5: 0.566; 1: 250; 2: 0.398; 3: 0.389; 4: 0.394; 5: 0.297; 1: 125; 2: 0.263; 3: 0.254; 4: 0.259; 5: 0.162; 1: 62.5; 2: 0.177; 3: 0.169; 4: 0.173; 5: 0.076; 1: 31.25; 2: 0.135; 3: 0.129; 4: 0.132; 5: 0.035; 1: 0; 2: 0.096; 3: 0.097; 4: 0.097 |
| ELISA Data Analysis | ELISA Standard Curve & Curve Expert software |
| Shelf Life | 6 months |
| Description | This Bovine IGF2 ELISA Kit was designed for the quantitative measurement of Bovine IGF2 protein in serum, plasma. It is a Sandwich ELISA kit, its detection range is 31.25 ng/mL-2000 ng/mL and the sensitivity is 7.81 ng/mL. |
MDSD
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