Human TAR DNA-binding protein 43 (TARDBP/TDP43) ELISA kit CSB-E17007h CUSABIO
$50.00
In stock
SKU
CSB-E17007h
Product Details
| Target Name | TAR DNA binding protein |
| Uniprot No. | Q13148 |
| Species | Homo sapiens (Human) |
| Storage | Store at 2-8°C. Please refer to protocol. |
| Sensitivity | 0.078 ng/mL |
| Troubleshooting and FAQs | ELISA kit FAQs |
| Lead Time | 3-5 working days after you place the order, and it takes another 3-5 days for delivery via DHL or FedEx. |
| Associated names | ALS10 ELISA Kit; OTTHUMP00000002171 ELISA Kit; OTTHUMP00000002172 ELISA Kit; OTTHUMP00000002173 ELISA Kit; TADBP_HUMAN ELISA Kit; TAR DNA binding protein 43 ELISA Kit; TAR DNA binding protein ELISA Kit; TAR DNA-binding protein 43 ELISA Kit; TARDBP ELISA Kit; TDP 43 ELISA Kit; TDP-43 ELISA Kit; TDP43 ELISA Kit |
| Abbreviation | TARDBP |
| Sample Types | serum, plasma, cell culture supernates, cerebrospinal fluid (CSF) |
| Detection Range | 0.312 ng/mL-20 ng/mL |
| Assay Time | 1-5h |
| Sample Loading Volume | 50-100ul |
| Detection Wavelength | 450 nm |
| Research Area | Others |
| Assay Principle | quantitative |
| Measurement | Sandwich |
| Precision | headers: Intra-assay Precision (Precision within an assay): CV%<8%; rows: Intra-assay Precision (Precision within an assay): CV%<8%: Intra-assay Precision (Precision within an assay): CV%<8%; 1: Three samples of known concentration were tested twenty times on one plate to assess.; Intra-assay Precision (Precision within an assay): CV%<8%: Inter-assay Precision (Precision between assays): CV%<10%; 1: Three samples of known concentration were tested in twenty assays to assess.; |
| Linearity | headers: To assess the linearity of the assay, samples were spiked with high concentrations of human TDP43 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.; rows: To assess the linearity of the assay, samples were spiked with high concentrations of human TDP43 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.: To assess the linearity of the assay, samples were spiked with high concentrations of human TDP43 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.; 2: Sample; 3: Serum(n=4); 1: 1:1; 2: Average %; 3: 86; 1: Range %; 2: 80-93; 1: 1:2; 2: Average %; 3: 100; 1: Range %; 2: 95-104; 1: 1:4; 2: Average %; 3: 92; 1: Range %; 2: 88-95; 1: 1:8; 2: Average %; 3: 97; 1: Range %; 2: 90-104 |
| Recovery | headers: The recovery of human TDP43 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.; rows: The recovery of human TDP43 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.: The recovery of human TDP43 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.; 1: Sample Type; 2: Average % Recovery; 3: Range; 1: Serum (n=5); 2: 98; 3: 91-104; 1: EDTA plasma (n=4); 2: 96; 3: 90-102; ; |
| Typical Data | headers: These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.; rows: These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.: These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.; 1: ng/ml OD1 OD2 Average Corrected 20 2.360 2.296 2.328 2.201 10 1.568 1.570 1.569 1.442 5 1.026 1.061 1.044 0.917 2.5 0.653 0.679 0.666 0.539 1.25 0.433 0.459 0.446 0.319 0.625 0.304 0.321 0.313 0.186 0.312 0.219 0.223 0.221 0.094 0 0.128 0.126 0.127; 2: ng/ml; 3: OD1; 4: OD2; 5: Average; 6: Corrected; 8: 20; 9: 2.360; 10: 2.296; 11: 2.328; 12: 2.201; 14: 10; 15: 1.568; 16: 1.570; 17: 1.569; 18: 1.442; 20: 5; 21: 1.026; 22: 1.061; 23: 1.044; 24: 0.917; 26: 2.5; 27: 0.653; 28: 0.679; 29: 0.666; 30: 0.539; 32: 1.25; 33: 0.433; 34: 0.459; 35: 0.446; 36: 0.319; 38: 0.625; 39: 0.304; 40: 0.321; 41: 0.313; 42: 0.186; 44: 0.312; 45: 0.219; 46: 0.223; 47: 0.221; 48: 0.094; 50: 0; 51: 0.128; 52: 0.126; 53: 0.127; 1: ng/ml; 2: OD1; 3: OD2; 4: Average; 5: Corrected; 1: 20; 2: 2.360; 3: 2.296; 4: 2.328; 5: 2.201; 1: 10; 2: 1.568; 3: 1.570; 4: 1.569; 5: 1.442; 1: 5; 2: 1.026; 3: 1.061; 4: 1.044; 5: 0.917; 1: 2.5; 2: 0.653; 3: 0.679; 4: 0.666; 5: 0.539; 1: 1.25; 2: 0.433; 3: 0.459; 4: 0.446; 5: 0.319; 1: 0.625; 2: 0.304; 3: 0.321; 4: 0.313; 5: 0.186; 1: 0.312; 2: 0.219; 3: 0.223; 4: 0.221; 5: 0.094; 1: 0; 2: 0.128; 3: 0.126; 4: 0.127 |
| Materials provided | A micro ELISA plate --- The 96-well plate has been pre-coated with an anti-human TDP43 antibody. This dismountable microplate can be divided into 12 x 8 strip plates. Two vials lyophilized standard ---Dilute a bottle of the standard at dilution series, read the OD values, and then draw a standard curve. One vial Biotin-labeled TDP43 antibody (100 x concentrate) (120 μl/bottle) ---Act as the detection antibody. One vial HRP-avidin (100 x concentrate) (120 μl/bottle) ---Bind to the detection antibody and react with the TMB substrate to make the solution chromogenic. One vial Biotin-antibody Diluent (15 ml/bottle) ---Dilute the Biotin-antibody. One vial HRP-avidin Diluent (15 ml/bottle) ---Dilute the HRP-avidin solution. One vial Sample Diluent (50 ml/bottle)---Dilute the sample to an appropriate concentration. One vial Wash Buffer (25 x concentrate) (20 ml/bottle) ---Wash away unbound or free substances. One vial TMB Substrate (10 ml/bottle) ---Act as the chromogenic agent. TMB interacts with HRP, eliciting the solution turns blue. One vial Stop Solution (10 ml/bottle) ---Stop the color reaction. The solution color immediately turns from blue to yellow. Four Adhesive Strips (For 96 wells) --- Cover the microplate when incubation. An instruction manual |
| Materials not provided | A microplate reader capable of measuring absorbance at 450 nm, with the correction wavelength set at 540 nm or 570 nm. An incubator can provide stable incubation conditions up to 37°C±5°C. Centrifuge Vortex Squirt bottle, manifold dispenser, or automated microplate washer Absorbent paper for blotting the microtiter plate 50-300ul multi-channel micropipette Pipette tips Single-channel micropipette with different ranges 100ml and 500ml graduated cylinders Deionized or distilled water Timer Test tubes for dilution |
| ELISA Data Analysis | ELISA Standard Curve & Curve Expert software |
| Shelf Life | 6 months |
| Description | TAR DNA-binding protein 43 (TARDBP/TDP-43) is a highly conserved nuclear protein that plays essential roles in RNA metabolism, including transcriptional regulation, RNA splicing, and mRNA transport. Under normal physiological conditions, TDP-43 predominantly localizes to the nucleus where it binds to TAR DNA sequences and regulates gene expression. This protein has attracted considerable research attention due to its involvement in neurodegenerative diseases. Pathological aggregation and mislocalization of TDP-43 from the nucleus to the cytoplasm represents a hallmark feature of several proteinopathies, making it an important biomarker for neurological research. The Human TAR DNA-binding protein 43 ELISA kit (CSB-E17007h) uses a quantitative sandwich measurement principle for detecting TARDBP in human samples. This assay accommodates multiple sample types including serum, plasma, cell culture supernatants, and cerebrospinal fluid. The detection range spans 0.312 ng/mL to 20 ng/mL with sensitivity of 0.078 ng/mL. The protocol requires 50-100 μL sample volume and can be completed within 1-5 hours, with detection performed at 450 nm wavelength. Application Examples Note: The following application examples are drawn from a selection of publications citing this product. For additional applications, please refer to the full list of references in the "Citations" section. Based on the provided information, no citation excerpts were available to document specific research applications for this ELISA kit targeting TAR DNA binding protein in human samples. Without published research citations, it is not possible to provide a summary of validated applications or research contexts where this kit has been used in scientific studies. |
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