Bovine Interleukin 10,IL-10 ELISA Kit CSB-E12917B CUSABIO
$50.00
In stock
SKU
CSB-E12917B
Product Details
| Target Name | interleukin 10 |
| Uniprot No. | P43480 |
| Species | Bos taurus (Bovine) |
| Storage | Store at 2-8°C. Please refer to protocol. |
| Sensitivity | 2.5 pg/mL |
| Troubleshooting and FAQs | ELISA kit FAQs |
| Lead Time | 3-5 working days after you place the order, and it takes another 3-5 days for delivery via DHL or FedEx. |
| Associated names | IL10Interleukin-10 ELISA Kit; IL-10 ELISA Kit; Cytokine synthesis inhibitory factor ELISA Kit; CSIF ELISA Kit |
| Abbreviation | IL10 |
| Sample Types | serum, plasma, tissue homogenates |
| Detection Range | 5 pg/mL-1000 pg/mL |
| Assay Time | 1-5h |
| Sample Loading Volume | 50-100ul |
| Detection Wavelength | 450 nm |
| Research Area | Immunology |
| Assay Principle | quantitative |
| Measurement | Competitive |
| Precision | headers: Intra-assay Precision (Precision within an assay): CV%<15%; rows: Intra-assay Precision (Precision within an assay): CV%<15%: Intra-assay Precision (Precision within an assay): CV%<15%; 1: Three samples of known concentration were tested twenty times on one plate to assess.; Intra-assay Precision (Precision within an assay): CV%<15%: Inter-assay Precision (Precision between assays): CV%<15%; 1: Three samples of known concentration were tested in twenty assays to assess.; |
| Linearity | headers: To assess the linearity of the assay, samples were spiked with high concentrations of bovine IL-10 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.; rows: To assess the linearity of the assay, samples were spiked with high concentrations of bovine IL-10 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.: To assess the linearity of the assay, samples were spiked with high concentrations of bovine IL-10 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.; 2: Sample; 3: Serum(n=4); 1: 1:1; 2: Average %; 3: 94; 1: Range %; 2: 89-98; 1: 1:2; 2: Average %; 3: 95; 1: Range %; 2: 91-98; 1: 1:4; 2: Average %; 3: 96; 1: Range %; 2: 87-102; 1: 1:8; 2: Average %; 3: 97; 1: Range %; 2: 91-104 |
| Recovery | headers: The recovery of bovine IL-10 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.; rows: The recovery of bovine IL-10 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.: The recovery of bovine IL-10 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.; 1: Sample Type; 2: Average % Recovery; 3: Range; 1: Serum (n=5); 2: 97; 3: 95-100; 1: EDTA plasma (n=4); 2: 96; 3: 92-103; ; |
| Typical Data | headers: These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.; rows: These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.: These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.; 1: pg/ml OD1 OD2 Average 1000 0.098 0.095 0.097 250 0.301 0.285 0.293 80 0.487 0.458 0.473 20 0.972 0.975 0.974 5 1.364 1.316 1.340 0 2.128 2.211 2.170; 2: pg/ml; 3: OD1; 4: OD2; 5: Average; 8: 1000; 9: 0.098; 10: 0.095; 11: 0.097; 14: 250; 15: 0.301; 16: 0.285; 17: 0.293; 20: 80; 21: 0.487; 22: 0.458; 23: 0.473; 26: 20; 27: 0.972; 28: 0.975; 29: 0.974; 32: 5; 33: 1.364; 34: 1.316; 35: 1.340; 38: 0; 39: 2.128; 40: 2.211; 41: 2.170; 1: pg/ml; 2: OD1; 3: OD2; 4: Average; 1: 1000; 2: 0.098; 3: 0.095; 4: 0.097; 1: 250; 2: 0.301; 3: 0.285; 4: 0.293; 1: 80; 2: 0.487; 3: 0.458; 4: 0.473; 1: 20; 2: 0.972; 3: 0.975; 4: 0.974; 1: 5; 2: 1.364; 3: 1.316; 4: 1.340; 1: 0; 2: 2.128; 3: 2.211; 4: 2.170 |
| ELISA Data Analysis | ELISA Standard Curve & Curve Expert software |
| Shelf Life | 6 months |
| Description | Interleukin 10 (IL-10) is a potent anti-inflammatory cytokine that plays a key role in immune regulation and homeostasis. Regulatory T cells, macrophages, and B cells produce IL-10, which suppresses inflammatory responses by inhibiting pro-inflammatory cytokine production and limiting antigen presentation. This immunosuppressive activity makes IL-10 essential for preventing excessive immune activation. It also maintains tissue integrity during inflammatory processes. Research into IL-10 levels provides valuable insights into immune dysfunction, autoimmune diseases, and inflammatory conditions across various species. The Bovine Interleukin 10, IL-10 ELISA Kit (CSB-E12917B) is designed for quantitative measurement of IL-10 in Bos taurus samples. This competitive assay works with serum, plasma, and tissue homogenates. The detection range spans 5 pg/mL to 1000 pg/mL with sensitivity of 2.5 pg/mL. The assay requires 50-100 μL sample volume and can be completed within 1-5 hours. Detection occurs at 450 nm wavelength, providing researchers with a reliable tool for bovine IL-10 quantification in immunological studies. Application Examples Note: The following application examples are drawn from a selection of publications citing this product. For additional applications, please refer to the full list of references in the "Citations" section. This bovine interleukin 10 ELISA kit has been used in research investigating immune responses and inflammatory processes in cattle. The kit allows quantitative measurement of IL-10 levels in various biological samples to determine anti-inflammatory cytokine profiles and immune system function. • Immune response studies: Measurement of immunological parameters alongside other immune markers including immunoglobulins and pro-inflammatory cytokines in serum samples • Antioxidant and inflammation research: Analysis of anti-inflammatory cytokine levels in conjunction with oxidative stress markers to understand the relationship between antioxidant capacity and immune function • Pro-inflammatory response analysis: Measurement of anti-inflammatory cytokines in plasma samples as part of comprehensive cytokine profiling studies • Tissue-based cytokine analysis: Quantification of IL-10 levels in lymph node tissue samples for localized immune response studies |
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