Human Thiamin pyrophosphokinase 1(TPK1) ELISA kit CSB-EL024103HU CUSABIO
$50.00
In stock
SKU
CSB-EL024103HU
Product Details
| Target Name | thiamin pyrophosphokinase 1 |
| Uniprot No. | Q9H3S4 |
| Species | Homo sapiens (Human) |
| Storage | Store at 2-8°C. Please refer to protocol. |
| Sensitivity | 7.8 pg/mL |
| Troubleshooting and FAQs | ELISA kit FAQs |
| Lead Time | 3-5 working days after you place the order, and it takes another 3-5 days for delivery via DHL or FedEx. |
| Associated names | hTPK1 ELISA Kit; Placental protein 20 ELISA Kit; PP20 ELISA Kit; Thiamin pyrophosphokinase 1 ELISA Kit; Thiamine diphosphokinase ELISA Kit; Thiamine kinase ELISA Kit; Thiamine pyrophosphokinase 1 ELISA Kit; THMD5 ELISA Kit; TPK1 ELISA Kit; TPK1_HUMAN ELISA Kit |
| Abbreviation | TPK1 |
| Sample Types | serum, plasma, tissue homogenates, cell lysates |
| Detection Range | 31.25 pg/mL-2000 pg/mL |
| Assay Time | 1-5h |
| Sample Loading Volume | 50-100ul |
| Detection Wavelength | 450 nm |
| Research Area | Others |
| Assay Principle | quantitative |
| Measurement | Sandwich |
| Precision | headers: Intra-assay Precision (Precision within an assay): CV%<8%; rows: Intra-assay Precision (Precision within an assay): CV%<8%: Intra-assay Precision (Precision within an assay): CV%<8%; 1: Three samples of known concentration were tested twenty times on one plate to assess.; Intra-assay Precision (Precision within an assay): CV%<8%: Inter-assay Precision (Precision between assays): CV%<10%; 1: Three samples of known concentration were tested in twenty assays to assess.; |
| Linearity | headers: To assess the linearity of the assay, samples were spiked with high concentrations of human TPK1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.; rows: To assess the linearity of the assay, samples were spiked with high concentrations of human TPK1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.: To assess the linearity of the assay, samples were spiked with high concentrations of human TPK1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.; 2: Sample; 3: Serum(n=4); 1: 1:1; 2: Average %; 3: 91; 1: Range %; 2: 86-95; 1: 1:2; 2: Average %; 3: 102; 1: Range %; 2: 97-107; 1: 1:4; 2: Average %; 3: 91; 1: Range %; 2: 85-97; 1: 1:8; 2: Average %; 3: 97; 1: Range %; 2: 91-103 |
| Recovery | headers: The recovery of human TPK1 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.; rows: The recovery of human TPK1 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.: The recovery of human TPK1 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.; 1: Sample Type; 2: Average % Recovery; 3: Range; 1: Serum (n=5); 2: 95; 3: 89-98; 1: EDTA plasma (n=4); 2: 97; 3: 90-100; ; |
| Typical Data | headers: These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.; rows: These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.: These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.; 1: pg/ml OD1 OD2 Average Corrected 2000 2.329 2.368 2.349 2.243 1000 1.876 1.857 1.867 1.761 500 1.339 1.368 1.354 1.248 250 0.843 0.839 0.841 0.735 125 0.566 0.599 0.583 0.477 62.5 0.411 0.423 0.417 0.311 31.25 0.250 0.259 0.255 0.149 0 0.105 0.107 0.106; 2: pg/ml; 3: OD1; 4: OD2; 5: Average; 6: Corrected; 8: 2000; 9: 2.329; 10: 2.368; 11: 2.349; 12: 2.243; 14: 1000; 15: 1.876; 16: 1.857; 17: 1.867; 18: 1.761; 20: 500; 21: 1.339; 22: 1.368; 23: 1.354; 24: 1.248; 26: 250; 27: 0.843; 28: 0.839; 29: 0.841; 30: 0.735; 32: 125; 33: 0.566; 34: 0.599; 35: 0.583; 36: 0.477; 38: 62.5; 39: 0.411; 40: 0.423; 41: 0.417; 42: 0.311; 44: 31.25; 45: 0.250; 46: 0.259; 47: 0.255; 48: 0.149; 50: 0; 51: 0.105; 52: 0.107; 53: 0.106; 1: pg/ml; 2: OD1; 3: OD2; 4: Average; 5: Corrected; 1: 2000; 2: 2.329; 3: 2.368; 4: 2.349; 5: 2.243; 1: 1000; 2: 1.876; 3: 1.857; 4: 1.867; 5: 1.761; 1: 500; 2: 1.339; 3: 1.368; 4: 1.354; 5: 1.248; 1: 250; 2: 0.843; 3: 0.839; 4: 0.841; 5: 0.735; 1: 125; 2: 0.566; 3: 0.599; 4: 0.583; 5: 0.477; 1: 62.5; 2: 0.411; 3: 0.423; 4: 0.417; 5: 0.311; 1: 31.25; 2: 0.250; 3: 0.259; 4: 0.255; 5: 0.149; 1: 0; 2: 0.105; 3: 0.107; 4: 0.106 |
| ELISA Data Analysis | ELISA Standard Curve & Curve Expert software |
| Shelf Life | 6 months |
| Description | This Human TPK1 ELISA Kit was designed for the quantitative measurement of Human TPK1 protein in serum, plasma, tissue homogenates, cell lysates. It is a Sandwich ELISA kit, its detection range is 31.25 pg/mL-2000 pg/mL and the sensitivity is 7.8 pg/mL. |
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