Human Thrombin ELISA Kit CSB-E14235h CUSABIO
$50.00
In stock
SKU
CSB-E14235h
Product Details
| Target Name | Thrombin |
| Uniprot No. | P00734 |
| Species | Homo sapiens (Human) |
| Storage | Store at 2-8°C. Please refer to protocol. |
| Sensitivity | 1.95 ng/mL |
| Troubleshooting and FAQs | ELISA kit FAQs |
| Lead Time | 3-5 working days after you place the order, and it takes another 3-5 days for delivery via DHL or FedEx. |
| Associated names | coagulation factor II (thrombin) ELISA Kit; Coagulation factor II ELISA Kit; F2 ELISA Kit; Factor II ELISA Kit; Prepro coagulation factor II ELISA Kit; Prothrombin ELISA Kit; prothrombin B-chain ELISA Kit; PT ELISA Kit; RPRGL2 ELISA Kit; serine protease ELISA Kit; THPH1 ELISA Kit; THRB ELISA Kit; THRB_HUMAN ELISA Kit; Thrombin heavy chain ELISA Kit |
| Abbreviation | Thrombin |
| Sample Types | serum, plasma |
| Detection Range | 7.8 ng/mL-500 ng/mL |
| Assay Time | 1-5h |
| Sample Loading Volume | 50-100ul |
| Detection Wavelength | 450 nm |
| Research Area | Cardiovascular |
| Assay Principle | quantitative |
| Measurement | Sandwich |
| Precision | headers: Intra-assay Precision (Precision within an assay): CV%<8%; rows: Intra-assay Precision (Precision within an assay): CV%<8%: Intra-assay Precision (Precision within an assay): CV%<8%; 1: Three samples of known concentration were tested twenty times on one plate to assess.; Intra-assay Precision (Precision within an assay): CV%<8%: Inter-assay Precision (Precision between assays): CV%<10%; 1: Three samples of known concentration were tested in twenty assays to assess.; |
| Linearity | headers: To assess the linearity of the assay, samples were spiked with high concentrations of human Thrombin in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.; rows: To assess the linearity of the assay, samples were spiked with high concentrations of human Thrombin in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.: To assess the linearity of the assay, samples were spiked with high concentrations of human Thrombin in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.; 2: Sample; 3: Serum(n=4); 1: 1:1000; 2: Average %; 3: 87; 1: Range %; 2: 80-95; 1: 1:2000; 2: Average %; 3: 97; 1: Range %; 2: 91-105; 1: 1:4000; 2: Average %; 3: 97; 1: Range %; 2: 92-104; 1: 1:8000; 2: Average %; 3: 95; 1: Range %; 2: 86-101 |
| Recovery | headers: The recovery of human Thrombin spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.; rows: The recovery of human Thrombin spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.: The recovery of human Thrombin spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.; 1: Sample Type; 2: Average % Recovery; 3: Range; 1: Serum (n=5); 2: 94; 3: 89-98; 1: EDTA plasma (n=4); 2: 97; 3: 90-109; ; |
| Typical Data | headers: These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.; rows: These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.: These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.; 1: ng/ml OD1 OD2 Average Corrected 500 2.771 2.806 2.789 2.656 250 2.347 2.357 2.352 2.219 125 1.631 1.695 1.663 1.530 62.5 1.056 1.104 1.080 0.947 31.2 0.591 0.576 0.584 0.451 15.6 0.373 0.389 0.381 0.248 7.8 0.285 0.296 0.291 0.158 0 0.134 0.132 0.133; 2: ng/ml; 3: OD1; 4: OD2; 5: Average; 6: Corrected; 8: 500; 9: 2.771; 10: 2.806; 11: 2.789; 12: 2.656; 14: 250; 15: 2.347; 16: 2.357; 17: 2.352; 18: 2.219; 20: 125; 21: 1.631; 22: 1.695; 23: 1.663; 24: 1.530; 26: 62.5; 27: 1.056; 28: 1.104; 29: 1.080; 30: 0.947; 32: 31.2; 33: 0.591; 34: 0.576; 35: 0.584; 36: 0.451; 38: 15.6; 39: 0.373; 40: 0.389; 41: 0.381; 42: 0.248; 44: 7.8; 45: 0.285; 46: 0.296; 47: 0.291; 48: 0.158; 50: 0; 51: 0.134; 52: 0.132; 53: 0.133; 1: ng/ml; 2: OD1; 3: OD2; 4: Average; 5: Corrected; 1: 500; 2: 2.771; 3: 2.806; 4: 2.789; 5: 2.656; 1: 250; 2: 2.347; 3: 2.357; 4: 2.352; 5: 2.219; 1: 125; 2: 1.631; 3: 1.695; 4: 1.663; 5: 1.530; 1: 62.5; 2: 1.056; 3: 1.104; 4: 1.080; 5: 0.947; 1: 31.2; 2: 0.591; 3: 0.576; 4: 0.584; 5: 0.451; 1: 15.6; 2: 0.373; 3: 0.389; 4: 0.381; 5: 0.248; 1: 7.8; 2: 0.285; 3: 0.296; 4: 0.291; 5: 0.158; 1: 0; 2: 0.134; 3: 0.132; 4: 0.133 |
| ELISA Data Analysis | ELISA Standard Curve & Curve Expert software |
| Shelf Life | 6 months |
| Description | This Human Thrombin ELISA Kit was designed for the quantitative measurement of Human Thrombin protein in serum, plasma. It is a Sandwich ELISA kit, its detection range is 7.8 ng/mL-500 ng/mL and the sensitivity is 1.95 ng/mL . |
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