Human Transforming Growth factor β1,TGF-β1 ELISA kit CSB-E04725h-IS CUSABIO
$50.00
In stock
SKU
CSB-E04725h-IS
Product Details
| Uniprot No. | P01137 |
| Species | Homo sapiens (Human) |
| Storage | Store at 2-8°C. Please refer to protocol. |
| Sensitivity | 5.8 pg/ml |
| Troubleshooting and FAQs | ELISA kit FAQs |
| Lead Time | 3-5 working days after you place the order, and it takes another 3-5 days for delivery via DHL or FedEx. |
| Associated names | Cartilage-inducing factor ELISA Kit; CED ELISA Kit; Differentiation inhibiting factor ELISA Kit; DPD1 ELISA Kit; LAP ELISA Kit; Latency-associated peptide ELISA Kit; Prepro transforming growth factor beta 1 ELISA Kit; TGF beta 1 ELISA Kit; TGF beta ELISA Kit; TGF beta 1 protein ELISA Kit; TGF-beta 1 protein ELISA Kit; TGF-beta-1 ELISA Kit; TGF-beta-5 ELISA Kit; TGF-beta1 ELISA Kit; TGFB ELISA Kit; Tgfb-1 ELISA Kit; tgfb1 ELISA Kit; TGFB1_HUMAN ELISA Kit; TGFbeta ELISA Kit; TGFbeta1 ELISA Kit; Transforming Growth Factor b1 ELISA Kit; Transforming Growth Factor beta 1 ELISA Kit; Transforming growth factor beta 1a ELISA Kit; transforming growth factor beta-1 ELISA Kit; transforming growth factor, beta 1 ELISA Kit |
| Abbreviation | TGFB1 |
| Sample Types | serum, plasma, cell culture supernates |
| Detection Range | 23.5 pg/ml-1500 pg/ml |
| Assay Time | 1-5h |
| Sample Loading Volume | 50-100ul |
| Detection Wavelength | 450 nm |
| Research Area | Signal Transduction |
| Assay Principle | quantitative |
| Measurement | Sandwich |
| Precision | headers: Intra-assay Precision (Precision within an assay): CV%<8%; rows: Intra-assay Precision (Precision within an assay): CV%<8%: Intra-assay Precision (Precision within an assay): CV%<8%; 1: Three samples of known concentration were tested twenty times on one plate to assess.; Intra-assay Precision (Precision within an assay): CV%<8%: Inter-assay Precision (Precision between assays): CV%<10%; 1: Three samples of known concentration were tested in twenty assays to assess. |
| Linearity | headers: To assess the linearity of the assay, samples were spiked with high concentrations of human TGF-β1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.; rows: To assess the linearity of the assay, samples were spiked with high concentrations of human TGF-β1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.: To assess the linearity of the assay, samples were spiked with high concentrations of human TGF-β1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.; 2: Sample; 3: Serum(n=4); 1: 1:50; 2: Average %; 3: 99; 1: Range %; 2: 92-106; 1: 1:100; 2: Average %; 3: 92; 1: Range %; 2: 85-99; 1: 1:200; 2: Average %; 3: 89; 1: Range %; 2: 84-94; 1: 1:400; 2: Average %; 3: 92; 1: Range %; 2: 86-98 |
| Recovery | headers: The recovery of human TGF-β1 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.; rows: The recovery of human TGF-β1 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.: The recovery of human TGF-β1 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.; 1: Sample Type; 2: Average % Recovery; 3: Range; 1: Serum (n=5); 2: 100; 3: 95-106; 1: EDTA plasma (n=4); 2: 93; 3: 88-99 |
| Typical Data | headers: These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.; rows: These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.: These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.; ; ; 1: pg/ml; 2: OD1; 3: OD2; 4: Average; 5: Corrected; 1: 1500; 2: 2.690; 3: 2.638; 4: 2.664; 5: 2.491; 1: 750; 2: 2.196; 3: 2.147; 4: 2.171; 5: 1.999; 1: 375; 2: 1.630; 3: 1.608; 4: 1.619; 5: 1.446; 1: 187.5; 2: 1.061; 3: 1.039; 4: 1.050; 5: 0.877; 1: 94; 2: 0.602; 3: 0.631; 4: 0.617; 5: 0.444; 1: 47; 2: 0.428; 3: 0.403; 4: 0.416; 5: 0.243; 1: 23.5; 2: 0.246; 3: 0.234; 4: 0.240; 5: 0.067; 1: 0; 2: 0.175; 3: 0.171; 4: 0.173 |
| ELISA Data Analysis | ELISA Standard Curve & Curve Expert software |
| Shelf Life | 6 months |
| Description | This ELISA kit provides quantitative measurement of TGF-β1 in human serum, plasma, and cell culture supernatants, supporting research into its central role in immune regulation, fibrosis, and growth signaling. Because TGF-β1 circulates in latent complexes and is released at widely varying levels depending on activation state and disease context, accurate quantification demands a kit calibrated to biologically meaningful concentrations. Detection Range: The 23.5–1500 pg/ml range spans the activated TGF-β1 concentrations typically observed in human plasma (low hundreds of pg/ml in healthy donors) through the elevated levels found in fibrotic, oncologic, and chronic inflammatory conditions, minimizing the need for extensive serial dilutions while capturing disease-relevant shifts. Sensitivity: At 5.8 pg/ml, the kit detects TGF-β1 in low-abundance samples such as conditioned media from regulatory T-cell cultures or early-stage fibrotic tissue explants where secreted levels may fall well below baseline plasma concentrations. Sample Compatibility: Validation across serum, plasma, and cell culture supernatants enables researchers to correlate circulating TGF-β1 biomarker levels with in vitro mechanistic studies of epithelial-mesenchymal transition or immunosuppressive signaling within a single assay platform. Assay Efficiency: A 50–100 µl sample requirement and 1–5 hour protocol accommodate limited primary cell harvests and time-sensitive multi-cytokine profiling experiments common in TGF-β1 research. This kit is well suited for studies in organ fibrosis progression, tumor immune evasion, and regulatory T-cell functional characterization. |
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