Bovine Interleukin 1β (IL- 1β) ELISA kit CSB-E12986B CUSABIO
$50.00
In stock
SKU
CSB-E12986B
Product Details
| Target Name | interleukin 1, beta |
| Uniprot No. | P09428 |
| Species | Bos taurus (Bovine) |
| Storage | Store at 2-8°C. Please refer to protocol. |
| Sensitivity | 15.6 pg/mL |
| Troubleshooting and FAQs | ELISA kit FAQs |
| Lead Time | 3-5 working days after you place the order, and it takes another 3-5 days for delivery via DHL or FedEx. |
| Associated names | IL1B ELISA Kit; Interleukin-1 beta ELISA Kit; IL-1 beta ELISA Kit |
| Abbreviation | IL1B |
| Sample Types | serum, plasma, tissue homogenates |
| Detection Range | 62.5 pg/mL-4000 pg/mL |
| Assay Time | 1-5h |
| Sample Loading Volume | 50-100ul |
| Detection Wavelength | 450 nm |
| Research Area | Immunology |
| Assay Principle | quantitative |
| Measurement | Sandwich |
| Precision | headers: Intra-assay Precision (Precision within an assay): CV%<8%; rows: Intra-assay Precision (Precision within an assay): CV%<8%: Intra-assay Precision (Precision within an assay): CV%<8%; 1: Three samples of known concentration were tested twenty times on one plate to assess.; Intra-assay Precision (Precision within an assay): CV%<8%: Inter-assay Precision (Precision between assays): CV%<10%; 1: Three samples of known concentration were tested in twenty assays to assess.; |
| Linearity | headers: To assess the linearity of the assay, samples were spiked with high concentrations of bovine IL-1β in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.; rows: To assess the linearity of the assay, samples were spiked with high concentrations of bovine IL-1β in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.: To assess the linearity of the assay, samples were spiked with high concentrations of bovine IL-1β in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.; 2: Sample; 3: Serum(n=4); 1: 1:1; 2: Average %; 3: 99; 1: Range %; 2: 95-108; 1: 1:2; 2: Average %; 3: 94; 1: Range %; 2: 91-98; 1: 1:4; 2: Average %; 3: 92; 1: Range %; 2: 88-95; 1: 1:8; 2: Average %; 3: 87; 1: Range %; 2: 81-93 |
| Recovery | headers: The recovery of bovine IL-1β spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.; rows: The recovery of bovine IL-1β spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.: The recovery of bovine IL-1β spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.; 1: Sample Type; 2: Average % Recovery; 3: Range; 1: Serum (n=5); 2: 95; 3: 90-102; 1: EDTA plasma (n=4); 2: 92; 3: 87-96; ; |
| Typical Data | headers: These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.; rows: These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.: These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.; 1: pg/ml OD1 OD2 Average Corrected 4000 2.876 2.862 2.869 2.751 2000 2.444 2.439 2.442 2.324 1000 1.903 1.856 1.880 1.762 500 1.412 1.403 1.408 1.290 250 1.164 1.185 1.175 1.057 125 0.809 0.799 0.804 0.686 62.5 0.471 0.479 0.475 0.357 0 0.117 0.118 0.118; 2: pg/ml; 3: OD1; 4: OD2; 5: Average; 6: Corrected; 8: 4000; 9: 2.876; 10: 2.862; 11: 2.869; 12: 2.751; 14: 2000; 15: 2.444; 16: 2.439; 17: 2.442; 18: 2.324; 20: 1000; 21: 1.903; 22: 1.856; 23: 1.880; 24: 1.762; 26: 500; 27: 1.412; 28: 1.403; 29: 1.408; 30: 1.290; 32: 250; 33: 1.164; 34: 1.185; 35: 1.175; 36: 1.057; 38: 125; 39: 0.809; 40: 0.799; 41: 0.804; 42: 0.686; 44: 62.5; 45: 0.471; 46: 0.479; 47: 0.475; 48: 0.357; 50: 0; 51: 0.117; 52: 0.118; 53: 0.118; 1: pg/ml; 2: OD1; 3: OD2; 4: Average; 5: Corrected; 1: 4000; 2: 2.876; 3: 2.862; 4: 2.869; 5: 2.751; 1: 2000; 2: 2.444; 3: 2.439; 4: 2.442; 5: 2.324; 1: 1000; 2: 1.903; 3: 1.856; 4: 1.880; 5: 1.762; 1: 500; 2: 1.412; 3: 1.403; 4: 1.408; 5: 1.290; 1: 250; 2: 1.164; 3: 1.185; 4: 1.175; 5: 1.057; 1: 125; 2: 0.809; 3: 0.799; 4: 0.804; 5: 0.686; 1: 62.5; 2: 0.471; 3: 0.479; 4: 0.475; 5: 0.357; 1: 0; 2: 0.117; 3: 0.118; 4: 0.118 |
| ELISA Data Analysis | ELISA Standard Curve & Curve Expert software |
| Shelf Life | 6 months |
| Description | This Bovine IL1B ELISA Kit was designed for the quantitative measurement of Bovine IL1B protein in serum, plasma, tissue homogenates. It is a Sandwich ELISA kit, its detection range is 62.5 pg/mL-4000 pg/mL and the sensitivity is 15.6 pg/mL . |
MDSD
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