Bovine Interleukin 2,IL-2 ELISA Kit CSB-E09810b-IS CUSABIO
$50.00
In stock
SKU
CSB-E09810b-IS
Product Details
| Target Name | interleukin 2 |
| Uniprot No. | P05016 |
| Species | Bos taurus (Bovine) |
| Storage | Store at 2-8°C. Please refer to protocol. |
| Sensitivity | 0.078ng/ml |
| Troubleshooting and FAQs | ELISA kit FAQs |
| Lead Time | 3-5 working days |
| Associated names | IL2 ELISA kit; IL-2Interleukin-2 ELISA kit; IL-2 ELISA kit; T-cell growth factor ELISA kit; TCGF ELISA kit |
| Abbreviation | IL2 |
| Sample Types | serum, plasma, tissue homogenates |
| Detection Range | 0.156ng/mL-10ng/mL |
| Assay Time | 1-5h |
| Sample Loading Volume | 50-100ul |
| Detection Wavelength | 450 nm |
| Research Area | Immunology |
| Assay Principle | quantitative |
| Measurement | Sandwich |
| Precision | headers: Intra-assay Precision (Precision within an assay): CV%<8%; rows: Intra-assay Precision (Precision within an assay): CV%<8%: Intra-assay Precision (Precision within an assay): CV%<8%; 1: Three samples of known concentration were tested twenty times on one plate to assess.; Intra-assay Precision (Precision within an assay): CV%<8%: Inter-assay Precision (Precision between assays): CV%<10%; 1: Three samples of known concentration were tested in twenty assays to assess. |
| Linearity | headers: To assess the linearity of the assay, samples were spiked with high concentrations of bovine IL-2 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.; rows: To assess the linearity of the assay, samples were spiked with high concentrations of bovine IL-2 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.: To assess the linearity of the assay, samples were spiked with high concentrations of bovine IL-2 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.; 2: Sample; 3: Serum(n=4); 1: 1:1; 2: Average %; 3: 92; 1: Range %; 2: 88-97; 1: 1:2; 2: Average %; 3: 99; 1: Range %; 2: 92-106; 1: 1:4; 2: Average %; 3: 94; 1: Range %; 2: 89-99; 1: 1:8; 2: Average %; 3: 101; 1: Range %; 2: 97-105 |
| Recovery | headers: The recovery of bovine IL-2 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.; rows: The recovery of bovine IL-2 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.: The recovery of bovine IL-2 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.; 1: Sample Type; 2: Average % Recovery; 3: Range; 1: Serum (n=5); 2: 102; 3: 95-109; 1: EDTA plasma (n=4); 2: 90; 3: 83-97 |
| Typical Data | headers: These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.; rows: These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.: These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.; ; 1: ng/ml; 2: OD1; 3: OD2; 4: Average; 5: Corrected; 1: 10; 2: 3.011; 3: 2.934; 4: 2.972; 5: 2.849; 1: 5; 2: 1.914; 3: 1.967; 4: 1.940; 5: 1.817; 1: 2.5; 2: 1.050; 3: 1.028; 4: 1.039; 5: 0.916; 1: 1.25; 2: 0.615; 3: 0.609; 4: 0.612; 5: 0.489; 1: 0.625; 2: 0.288; 3: 0.301; 4: 0.295; 5: 0.171; 1: 0.312; 2: 0.181; 3: 0.191; 4: 0.186; 5: 0.063; 1: 0.156; 2: 0.146; 3: 0.154; 4: 0.150; 5: 0.027; 1: 0; 2: 0.121; 3: 0.125; 4: 0.123 |
| ELISA Data Analysis | ELISA Standard Curve & Curve Expert software |
| Shelf Life | 6 months |
| Description | This ELISA kit provides quantitative measurement of interleukin-2 (IL-2) in bovine samples, supporting immunological research where T-cell activation and proliferative signaling are central to the study design. Because bovine IL-2 is a critical readout in vaccination trials, parasite challenge models, and mastitis immunology, this kit is engineered to capture the cytokine across both resting and stimulated conditions. Detection Range: The 0.156–10 ng/mL range spans from baseline circulating IL-2 levels through the elevated concentrations seen in mitogen-stimulated PBMC supernatants and inflamed tissue homogenates. Most activated bovine lymphocyte cultures produce IL-2 within this window, minimizing the need for sample dilution or concentration steps. Sensitivity: At 0.078 ng/mL, the kit detects IL-2 in unstimulated or weakly stimulated samples where secretion is minimal, such as early post-vaccination time points or naïve control conditions that still require quantifiable baseline values. Sample Compatibility: Validation across serum, plasma, and tissue homogenates enables paired systemic and local immune profiling — for example, comparing circulating IL-2 with mammary gland or lymph node tissue levels during infectious challenge studies. Assay Efficiency: The 1–5 hour protocol and 50–100 µL sample volume accommodate limited-volume calf blood draws and high-throughput screening of multiple vaccination cohorts within a single working day. This kit is directly applicable to bovine vaccine efficacy studies, Th1 immune response characterization, and infectious disease models including bovine tuberculosis and theileriosis. |
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