Human Triggering Receptor Expresses on Myeloid Cells-1,TREM-1 ELISA Kit CSB-E04836h CUSABIO
$50.00
In stock
SKU
CSB-E04836h
Product Details
| Target Name | triggering receptor expressed on myeloid cells 1 |
| Uniprot No. | Q9NP99 |
| Species | Homo sapiens (Human) |
| Storage | Store at 2-8°C. Please refer to protocol. |
| Sensitivity | 7.81 pg/mL |
| Troubleshooting and FAQs | ELISA kit FAQs |
| Lead Time | 3-5 working days after you place the order, and it takes another 3-5 days for delivery via DHL or FedEx. |
| Associated names | CD354 ELISA Kit; OTTMUSP00000018206 ELISA Kit; TREM 1 ELISA Kit; TREM-1 ELISA Kit; TREM1 ELISA Kit; TREM1_HUMAN ELISA Kit; Triggering receptor expressed on monocytes 1 ELISA Kit; Triggering receptor expressed on myeloid cells 1 ELISA Kit; Triggering receptor TREM 1 ELISA Kit; Triggering receptor TREM1 ELISA Kit |
| Abbreviation | TREM1 |
| Sample Types | serum, plasma, tissue homogenates |
| Detection Range | 31.25 pg/mL-2000 pg/mL |
| Assay Time | 1-5h |
| Sample Loading Volume | 50-100ul |
| Detection Wavelength | 450 nm |
| Research Area | Immunology |
| Assay Principle | quantitative |
| Measurement | Sandwich |
| Precision | headers: Intra-assay Precision (Precision within an assay): CV%<8%; rows: Intra-assay Precision (Precision within an assay): CV%<8%: Intra-assay Precision (Precision within an assay): CV%<8%; 1: Three samples of known concentration were tested twenty times on one plate to assess.; Intra-assay Precision (Precision within an assay): CV%<8%: Inter-assay Precision (Precision between assays): CV%<10%; 1: Three samples of known concentration were tested in twenty assays to assess.; |
| Linearity | headers: To assess the linearity of the assay, samples were spiked with high concentrations of human TREM-1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.; rows: To assess the linearity of the assay, samples were spiked with high concentrations of human TREM-1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.: To assess the linearity of the assay, samples were spiked with high concentrations of human TREM-1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.; 2: Sample; 3: Serum(n=4); 1: 1:1; 2: Average %; 3: 97; 1: Range %; 2: 92-102; 1: 1:2; 2: Average %; 3: 90; 1: Range %; 2: 82-97; 1: 1:4; 2: Average %; 3: 104; 1: Range %; 2: 99-110; 1: 1:8; 2: Average %; 3: 86; 1: Range %; 2: 82-93 |
| Recovery | headers: The recovery of human TREM-1 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.; rows: The recovery of human TREM-1 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.: The recovery of human TREM-1 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.; 1: Sample Type; 2: Average % Recovery; 3: Range; 1: Serum (n=5); 2: 90; 3: 84-96; 1: EDTA plasma (n=4); 2: 96; 3: 90-101; ; |
| Typical Data | headers: These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.; rows: These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.: These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.; 1: pg/ml OD1 OD2 Average Corrected 2000 2.529 2.752 2.641 2.538 1000 2.038 2.079 2.059 1.956 500 1.304 1.389 1.347 1.244 250 0.701 0.733 0.717 0.614 125 0.441 0.455 0.448 0.345 62.5 0.302 0.326 0.314 0.211 31.25 0.205 0.201 0.203 0.100 0 0.104 0.101 0.103; 2: pg/ml; 3: OD1; 4: OD2; 5: Average; 6: Corrected; 8: 2000; 9: 2.529; 10: 2.752; 11: 2.641; 12: 2.538; 14: 1000; 15: 2.038; 16: 2.079; 17: 2.059; 18: 1.956; 20: 500; 21: 1.304; 22: 1.389; 23: 1.347; 24: 1.244; 26: 250; 27: 0.701; 28: 0.733; 29: 0.717; 30: 0.614; 32: 125; 33: 0.441; 34: 0.455; 35: 0.448; 36: 0.345; 38: 62.5; 39: 0.302; 40: 0.326; 41: 0.314; 42: 0.211; 44: 31.25; 45: 0.205; 46: 0.201; 47: 0.203; 48: 0.100; 50: 0; 51: 0.104; 52: 0.101; 53: 0.103; 1: pg/ml; 2: OD1; 3: OD2; 4: Average; 5: Corrected; 1: 2000; 2: 2.529; 3: 2.752; 4: 2.641; 5: 2.538; 1: 1000; 2: 2.038; 3: 2.079; 4: 2.059; 5: 1.956; 1: 500; 2: 1.304; 3: 1.389; 4: 1.347; 5: 1.244; 1: 250; 2: 0.701; 3: 0.733; 4: 0.717; 5: 0.614; 1: 125; 2: 0.441; 3: 0.455; 4: 0.448; 5: 0.345; 1: 62.5; 2: 0.302; 3: 0.326; 4: 0.314; 5: 0.211; 1: 31.25; 2: 0.205; 3: 0.201; 4: 0.203; 5: 0.100; 1: 0; 2: 0.104; 3: 0.101; 4: 0.103 |
| ELISA Data Analysis | ELISA Standard Curve & Curve Expert software |
| Shelf Life | 6 months |
| Description | CUSABIO's human TREM1 ELISA kit is an in vitro enzyme-linked immunosorbent assay for quantitatively measuring human TREM1 in serum, plasma, or tissue homogenates. This assay uses the sandwich enzyme immunoassay technique in combination with the enzyme-substrate chromogenic reaction to quantify the analyte in the sample. The color develops positively to the amount of TREM1 in samples. The color intensity is measured at 450 nm via a microplate reader. TREM1, an activating receptor expressed on myeloid cells, has the synergistic ability to amplify the signaling of the TLR4 or TLR2, which can recognize components of a variety of microorganisms including bacteria, fungi, and viruses. TREM1 mainly augments inflammation during acute inflammation and is upregulated during sepsis and pulmonary infection. It has been recognized as a critical immunomodulator in several inflammatory diseases of both infectious and non-infectious etiologies such as inflammatory bowel disease, acute pancreatitis, gouty arthritis, and atherosclerosis. |
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