Human Tumor necrosis factor α,TNF-α ELISA KIT CSB-E04740h-IS CUSABIO
$50.00
In stock
SKU
CSB-E04740h-IS
Product Details
| Target Name | tumor necrosis factor (TNF superfamily, member 2) |
| Uniprot No. | P01375 |
| Species | Homo sapiens (Human) |
| Storage | Store at 2-8°C. Please refer to protocol. |
| Sensitivity | 1.95 pg/mL |
| Troubleshooting and FAQs | ELISA kit FAQs |
| Lead Time | 3-5 working days after you place the order, and it takes another 3-5 days for delivery via DHL or FedEx. |
| Associated names | APC1 ELISA Kit; APC1 protein ELISA Kit; Cachectin ELISA Kit; DIF ELISA Kit; Differentiation inducing factor ELISA Kit; Macrophage cytotoxic factor ELISA Kit; Tnf ELISA Kit; TNF superfamily member 2 ELISA Kit; TNF superfamily, member 2 ELISA Kit; TNF, macrophage derived ELISA Kit; TNF, monocyte derived ELISA Kit; TNF-a ELISA Kit; TNF-alpha ELISA Kit; TNFA ELISA Kit; TNFA_HUMAN ELISA Kit; TNFSF2 ELISA Kit; Tumor necrosis factor (TNF superfamily member 2) ELISA Kit; Tumor necrosis factor alpha ELISA Kit; Tumor necrosis factor ELISA Kit; Tumor necrosis factor ligand superfamily member 2 ELISA Kit; Tumor Necrosis Factor, Membrane Form ELISA Kit; Tumor necrosis factor, soluble form ELISA Kit |
| Abbreviation | TNF |
| Sample Types | cell culture supernates |
| Detection Range | 7.8 pg/mL-500 pg/mL |
| Assay Time | 1-5h |
| Sample Loading Volume | 50-100ul |
| Detection Wavelength | 450 nm |
| Research Area | Immunology |
| Assay Principle | quantitative |
| Measurement | Sandwich |
| Precision | headers: Intra-assay Precision (Precision within an assay): CV%<8%; rows: Intra-assay Precision (Precision within an assay): CV%<8%: Intra-assay Precision (Precision within an assay): CV%<8%; 1: Three samples of known concentration were tested twenty times on one plate to assess.; Intra-assay Precision (Precision within an assay): CV%<8%: Inter-assay Precision (Precision between assays): CV%<10%; 1: Three samples of known concentration were tested in twenty assays to assess. |
| Linearity | headers: To assess the linearity of the assay, samples were spiked with high concentrations of humanTNF-α in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.; rows: To assess the linearity of the assay, samples were spiked with high concentrations of humanTNF-α in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.: To assess the linearity of the assay, samples were spiked with high concentrations of humanTNF-α in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.; 2: Sample; 3: Cell culture supernates(n=4); 1: 1:1; 2: Average %; 3: 90; 1: Range %; 2: 86-94; 1: 1:2; 2: Average %; 3: 92; 1: Range %; 2: 89-97; 1: 1:4; 2: Average %; 3: 95; 1: Range %; 2: 91-99; 1: 1:8; 2: Average %; 3: 93; 1: Range %; 2: 86-102 |
| Recovery | headers: The recovery of humanTNF-α spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.; rows: The recovery of humanTNF-α spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.: The recovery of humanTNF-α spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.; 1: Sample Type; 2: Average % Recovery; 3: Range; 1: Cell culture supernates(n=5); 2: 98; 3: 94-103 |
| Typical Data | headers: These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.; These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.; rows: These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.: These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.; 1: These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.; ; 1: pg/ml; 2: OD1; 3: OD2; 4: Average; 5: Corrected; 1: 500; 2: 2.986; 3: 2.875; 4: 2.931; 5: 2.761; 1: 250; 2: 2.234; 3: 2.199; 4: 2.217; 5: 2.047; 1: 125; 2: 1.457; 3: 1.408; 4: 1.433; 5: 1.263; 1: 62.5; 2: 0.982; 3: 0.974; 4: 0.978; 5: 0.808; 1: 31.2; 2: 0.579; 3: 0.605; 4: 0.592; 5: 0.422; 1: 15.6; 2: 0.365; 3: 0.393; 4: 0.379; 5: 0.209; 1: 7.8; 2: 0.277; 3: 0.289; 4: 0.283; 5: 0.113; 1: 0; 2: 0.171; 3: 0.168; 4: 0.170 |
| ELISA Data Analysis | ELISA Standard Curve & Curve Expert software |
| Shelf Life | 6 months |
| Description | This ELISA kit provides quantitative measurement of human TNF (tumor necrosis factor) in research applications focused on immunology and inflammatory signaling. Because TNF is often secreted at low picomolar concentrations by stimulated immune cells in vitro, this kit is designed to capture biologically meaningful differences across activation conditions and treatment groups. Detection Range: The 7.8–500 pg/mL range aligns with TNF concentrations typically produced by LPS-stimulated monocytes, activated macrophages, and T cells in culture. Most stimulated cell supernatants fall within this window without dilution, though strongly activated macrophage cultures may require modest dilution to remain on-curve. Sensitivity: At 1.95 pg/mL, the kit detects TNF at levels produced by unstimulated or weakly activated immune cells, enabling measurement of baseline secretion and subtle changes caused by low-dose stimuli or anti-inflammatory compounds. Sample Compatibility: Validation for cell culture supernatants directly supports in vitro immunology workflows — including monocyte/macrophage polarization studies, T cell activation assays, and drug screening panels targeting NF-κB-dependent TNF release. Assay Efficiency: The 1–5 hour protocol and 50–100 µL sample volume accommodate experiments using primary human PBMCs or differentiated macrophages, where conditioned media volume is limited and multiple cytokines must be measured from the same collection. This kit is well-suited for studies in sepsis modeling, anti-TNF drug screening, and innate immune pathway characterization. |
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