Human Tumor necrosis factor soluble receptor Ⅰ,TNFsR-ⅠELISA KIT CSB-E04736h-IS CUSABIO
$50.00
In stock
SKU
CSB-E04736h-IS
Product Details
| Uniprot No. | P19438 |
| Species | Homo sapiens (Human) |
| Storage | Store at 2-8°C. Please refer to protocol. |
| Sensitivity | 1.95 pg/ml |
| Troubleshooting and FAQs | ELISA kit FAQs |
| Lead Time | 3-5 working days after you place the order, and it takes another 3-5 days for delivery via DHL or FedEx. |
| Associated names | CD120a ELISA Kit; FPF ELISA Kit; MGC19588 ELISA Kit; p55 ELISA Kit; p55-R ELISA Kit; p60 ELISA Kit; TBP1 ELISA Kit; TBPI ELISA Kit; TNF R ELISA Kit; TNF R55 ELISA Kit; TNF-R1 ELISA Kit; TNF-RI ELISA Kit; TNFAR ELISA Kit; TNFR-I ELISA Kit; TNFR1 ELISA Kit; TNFR55 ELISA Kit; TNFR60 ELISA Kit; TNFRI ELISA Kit; TNFRSF1a ELISA Kit; TNR1A_HUMAN ELISA Kit; Tumor necrosis factor receptor 1 ELISA Kit; Tumor necrosis factor receptor superfamily, member 1A ELISA Kit; Tumor necrosis factor receptor type 1 ELISA Kit; Tumor necrosis factor receptor type I ELISA Kit; Tumor necrosis factor-binding protein 1 ELISA Kit |
| Abbreviation | TNFRSF1A |
| Sample Types | serum, plasma, tissue homogenates, urine, saliva, cell culture supernates |
| Detection Range | 7.8 pg/ml-500 pg/ml |
| Assay Time | 1-5h |
| Sample Loading Volume | 50-100ul |
| Detection Wavelength | 450 nm |
| Research Area | Signal Transduction |
| Assay Principle | quantitative |
| Measurement | Sandwich |
| Precision | headers: Intra-assay Precision (Precision within an assay): CV%<8%; rows: Intra-assay Precision (Precision within an assay): CV%<8%: Intra-assay Precision (Precision within an assay): CV%<8%; 1: Three samples of known concentration were tested twenty times on one plate to assess.; Intra-assay Precision (Precision within an assay): CV%<8%: Inter-assay Precision (Precision between assays): CV%<10%; 1: Three samples of known concentration were tested in twenty assays to assess. |
| Linearity | headers: To assess the linearity of the assay, samples were spiked with high concentrations of human TNFsR-Ⅰ in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.; rows: To assess the linearity of the assay, samples were spiked with high concentrations of human TNFsR-Ⅰ in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.: To assess the linearity of the assay, samples were spiked with high concentrations of human TNFsR-Ⅰ in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.; 2: Sample; 3: Serum(n=4); 1: 1:4; 2: Average %; 3: 85; 1: Range %; 2: 80-92; 1: 1:8; 2: Average %; 3: 92; 1: Range %; 2: 87-98; 1: 1:16; 2: Average %; 3: 97; 1: Range %; 2: 90-105; 1: 1:32; 2: Average %; 3: 93; 1: Range %; 2: 86-98 |
| Recovery | headers: The recovery of human TNFsR-Ⅰspiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.; rows: The recovery of human TNFsR-Ⅰspiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.: The recovery of human TNFsR-Ⅰspiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.; 1: Sample Type; 2: Average % Recovery; 3: Range; 1: Serum (n=5); 2: 91; 3: 85-97; 1: EDTA plasma (n=4); 2: 96; 3: 90-103 |
| Typical Data | headers: These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.; rows: These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.: These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.; ; ; 1: pg/ml; 2: OD1; 3: OD2; 4: Average; 5: Corrected; 1: 500; 2: 3.009; 3: 2.904; 4: 2.956; 5: 2.783; 1: 250; 2: 2.282; 3: 2.167; 4: 2.225; 5: 2.052; 1: 125; 2: 1.430; 3: 1.428; 4: 1.429; 5: 1.256; 1: 62.5; 2: 0.869; 3: 0.849; 4: 0.859; 5: 0.686; 1: 31.2; 2: 0.537; 3: 0.521; 4: 0.529; 5: 0.356; 1: 15.6; 2: 0.401; 3: 0.393; 4: 0.397; 5: 0.224; 1: 7.8; 2: 0.283; 3: 0.289; 4: 0.286; 5: 0.113; 1: 0; 2: 0.171; 3: 0.175; 4: 0.173 |
| ELISA Data Analysis | ELISA Standard Curve & Curve Expert software |
| Shelf Life | 6 months |
| Description | This ELISA kit provides quantitative measurement of soluble TNFRSF1A (sTNFR1) in human samples, supporting research into TNF-α signaling and inflammatory pathway regulation. Because sTNFR1 is shed from cell surfaces during inflammation and serves as both a biomarker and a decoy receptor modulating TNF-α bioavailability, accurate quantification across biological compartments is essential for interpreting TNF signaling dynamics. Detection Range: The 7.8–500 pg/ml range captures circulating sTNFR1 levels in healthy serum (typically 1–2 ng/ml), meaning most serum and plasma samples will require dilution, while lower-abundance matrices like urine, saliva, and conditioned media can often be measured neat or with minimal preparation. Sensitivity: At 1.95 pg/ml, the kit detects sTNFR1 in low-concentration samples such as cell culture supernatants from unstimulated cells or saliva, where shed receptor levels may fall well below systemic concentrations. Sample Compatibility: Validation across serum, plasma, tissue homogenates, urine, saliva, and cell culture supernatants enables paired systemic-and-local measurements — for example, comparing circulating sTNFR1 with levels in inflamed tissue homogenates or renal clearance via urine in nephritis studies. Assay Efficiency: The 1–5 hour protocol and 50–100 µl sample volume accommodate experiments using limited primary cell supernatants or serial-sampled clinical specimens where material conservation is critical. This kit is particularly suited for studies in sepsis-associated immune dysregulation, anti-TNF therapy monitoring, and chronic inflammatory disease progression. |
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