Human Vascular endothelial cell growth factor receptor 2,VEGFR-2/Flk-1 ELISA kit CSB-E04763h-IS CUSABIO
$50.00
In stock
SKU
CSB-E04763h-IS
Product Details
| Target Name | kinase insert domain receptor (a type III receptor tyrosine kinase) |
| Uniprot No. | P35968 |
| Species | Homo sapiens (Human) |
| Storage | Store at 2-8°C. Please refer to protocol. |
| Sensitivity | 0.039ng/mL |
| Troubleshooting and FAQs | ELISA kit FAQs |
| Lead Time | 3-5 working days |
| Associated names | CD309 ELISA Kit; CD309 antigen ELISA Kit; EC 2.7.10.1 ELISA Kit; Fetal liver kinase 1 ELISA Kit; FLK-1 ELISA Kit; FLK1 ELISA Kit; FLK1, mouse, homolog of ELISA Kit; Kdr ELISA Kit; Kinase insert domain receptor (a type III receptor tyrosine kinase) ELISA Kit; Kinase insert domain receptor ELISA Kit; KRD1 ELISA Kit; Ly73 ELISA Kit; Protein tyrosine kinase receptor FLK1 ELISA Kit; Protein-tyrosine kinase receptor flk-1 ELISA Kit; soluble VEGFR2 ELISA Kit; Tyrosine kinase growth factor receptor ELISA Kit; Vascular endothelial growth factor receptor 2 ELISA Kit; VEGFR 2 ELISA Kit; VEGFR ELISA Kit; VEGFR-2 ELISA Kit; VEGFR2 ELISA Kit; VGFR2_HUMAN ELISA Kit |
| Abbreviation | VEGFR-2/Flk-1 |
| Sample Types | serum, plasma, tissue homogenates |
| Detection Range | 0.078ng/mL-5ng/mL |
| Assay Time | 1-5h |
| Sample Loading Volume | 50-100ul |
| Detection Wavelength | 450 nm |
| Research Area | Cardiovascular |
| Assay Principle | quantitative |
| Measurement | Sandwich |
| Precision | headers: Intra-assay Precision (Precision within an assay): CV%<8%; rows: Intra-assay Precision (Precision within an assay): CV%<8%: Intra-assay Precision (Precision within an assay): CV%<8%; 1: Three samples of known concentration were tested twenty times on one plate to assess.; Intra-assay Precision (Precision within an assay): CV%<8%: Inter-assay Precision (Precision between assays): CV%<10%; 1: Three samples of known concentration were tested in twenty assays to assess. |
| Linearity | headers: To assess the linearity of the assay, samples were spiked with high concentrations of human VEGFR-2/Flk-1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.; rows: To assess the linearity of the assay, samples were spiked with high concentrations of human VEGFR-2/Flk-1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.: To assess the linearity of the assay, samples were spiked with high concentrations of human VEGFR-2/Flk-1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.; 2: Sample; 3: Serum(n=4); 1: 1:1; 2: Average %; 3: 93; 1: Range %; 2: 89-98; 1: 1:2; 2: Average %; 3: 101; 1: Range %; 2: 96-107; 1: 1:4; 2: Average %; 3: 90; 1: Range %; 2: 86-96; 1: 1:8; 2: Average %; 3: 98; 1: Range %; 2: 91-103 |
| Recovery | headers: The recovery of human VEGFR-2/Flk-1 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.; rows: The recovery of human VEGFR-2/Flk-1 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.: The recovery of human VEGFR-2/Flk-1 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.; 1: Sample Type; 2: Average % Recovery; 3: Range; 1: Serum (n=5); 2: 91; 3: 86-96; 1: EDTA plasma (n=4); 2: 99; 3: 90-108 |
| Typical Data | headers: ; rows: ; 1: ng/ml; 2: OD1; 3: OD2; 4: Average; 5: Corrected; 1: 5; 2: 3.168; 3: 3.334; 4: 3.251; 5: 3.081; 1: 2.5; 2: 2.596; 3: 2.667; 4: 2.632; 5: 2.461; 1: 1.25; 2: 1.830; 3: 1.978; 4: 1.904; 5: 1.734; 1: 0.625; 2: 1.163; 3: 1.229; 4: 1.196; 5: 1.026; 1: 0.312; 2: 0.738; 3: 0.721; 4: 0.729; 5: 0.559; 1: 0.156; 2: 0.535; 3: 0.513; 4: 0.524; 5: 0.354; 1: 0.078; 2: 0.306; 3: 0.334; 4: 0.320; 5: 0.150; 1: 0; 2: 0.168; 3: 0.172; 4: 0.170 |
| ELISA Data Analysis | ELISA Standard Curve & Curve Expert software |
| Shelf Life | 6 months |
| Description | This ELISA kit provides quantitative measurement of soluble VEGFR-2/Flk-1 (sVEGFR-2) in human samples, supporting research into angiogenesis-driven cardiovascular pathology. Because circulating sVEGFR-2 levels shift in response to endothelial activation and anti-angiogenic therapy, researchers need an assay calibrated to capture both baseline and disease-modulated concentrations across translational sample types. Detection Range: The 0.078–5 ng/mL range aligns with reported circulating sVEGFR-2 concentrations in human plasma, which typically fall between 5–12 ng/mL in healthy adults and can decrease significantly during anti-VEGF treatment or heart failure progression. Most serum and plasma samples will require a modest 2–5× dilution, while tissue homogenates from ischemic or tumor-adjacent myocardium can generally be assayed with minimal adjustment. Sensitivity: At 0.039 ng/mL, the assay detects sVEGFR-2 in low-abundance contexts such as diluted plasma from patients on VEGF pathway inhibitors or conditioned media from endothelial cell cultures with suppressed receptor shedding. Sample Compatibility: Validation across serum, plasma, and tissue homogenates enables paired analyses linking circulating sVEGFR-2 to local receptor expression in ischemic or infarcted myocardial tissue within the same study. Assay Efficiency: The 1–5 hour protocol and 50–100 µL sample volume accommodate serial blood draws from longitudinal cardiovascular monitoring studies where repeated sampling limits available volume. This kit is well-suited for studies of myocardial ischemia, anti-angiogenic cardiotoxicity, and peripheral artery disease biomarker discovery. |
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