Human Vasoactive Intestinal Peptide,VIP ELISA Kit CSB-E08354h CUSABIO
$50.00
In stock
SKU
CSB-E08354h
Product Details
| Target Name | vasoactive intestinal peptide |
| Uniprot No. | P01282 |
| Species | Homo sapiens (Human) |
| Storage | Store at 2-8°C. Please refer to protocol. |
| Sensitivity | 3.9 pg/mL |
| Troubleshooting and FAQs | ELISA kit FAQs |
| Lead Time | 3-5 working days after you place the order, and it takes another 3-5 days for delivery via DHL or FedEx. |
| Associated names | Peptide histidine methioninamide 27 ELISA Kit; Peptide histidine valine 42 ELISA Kit; PHM27 ELISA Kit; PHV42 ELISA Kit; Prepro VIP ELISA Kit; Vasoactive intestinal peptide ELISA Kit; Vasoactive intestinal polypeptide ELISA Kit; VIP ELISA Kit; VIP peptides ELISA Kit; VIP peptides precursor ELISA Kit; VIP_HUMAN ELISA Kit |
| Abbreviation | VIP |
| Sample Types | serum, plasma, tissue homogenates |
| Detection Range | 15.6 pg/mL-1000 pg/mL |
| Assay Time | 1-5h |
| Sample Loading Volume | 50-100ul |
| Detection Wavelength | 450 nm |
| Research Area | Neuroscience |
| Assay Principle | quantitative |
| Measurement | Sandwich |
| Precision | headers: Intra-assay Precision (Precision within an assay): CV%<8%; rows: Intra-assay Precision (Precision within an assay): CV%<8%: Intra-assay Precision (Precision within an assay): CV%<8%; 1: Three samples of known concentration were tested twenty times on one plate to assess.; Intra-assay Precision (Precision within an assay): CV%<8%: Inter-assay Precision (Precision between assays): CV%<10%; 1: Three samples of known concentration were tested in twenty assays to assess.; |
| Linearity | headers: To assess the linearity of the assay, samples were spiked with high concentrations of human VIP in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.; rows: To assess the linearity of the assay, samples were spiked with high concentrations of human VIP in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.: To assess the linearity of the assay, samples were spiked with high concentrations of human VIP in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.; 2: Sample; 3: Serum(n=4); 1: 1:1; 2: Average %; 3: 87; 1: Range %; 2: 80-95; 1: 1:2; 2: Average %; 3: 95; 1: Range %; 2: 91-99; 1: 1:4; 2: Average %; 3: 99; 1: Range %; 2: 92-105; 1: 1:8; 2: Average %; 3: 92; 1: Range %; 2: 86-100 |
| Recovery | headers: The recovery of human VIP spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.; rows: The recovery of human VIP spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.: The recovery of human VIP spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.; 1: Sample Type; 2: Average % Recovery; 3: Range; 1: Serum (n=5); 2: 99; 3: 89-105; 1: EDTA plasma (n=4); 2: 94; 3: 90-98; ; |
| Typical Data | headers: These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.; rows: These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.: These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.; 1: pg/ml OD1 OD2 Average Corrected 1000 2.795 2.598 2.697 2.547 500 2.320 2.189 2.255 2.105 250 1.644 1.564 1.604 1.454 125 0.913 0.885 0.899 0.749 62.5 0.425 0.416 0.421 0.271 31.25 0.289 0.281 0.285 0.135 15.6 0.227 0.224 0.226 0.076 0 0.151 0.149 0.150; 2: pg/ml; 3: OD1; 4: OD2; 5: Average; 6: Corrected; 8: 1000; 9: 2.795; 10: 2.598; 11: 2.697; 12: 2.547; 14: 500; 15: 2.320; 16: 2.189; 17: 2.255; 18: 2.105; 20: 250; 21: 1.644; 22: 1.564; 23: 1.604; 24: 1.454; 26: 125; 27: 0.913; 28: 0.885; 29: 0.899; 30: 0.749; 32: 62.5; 33: 0.425; 34: 0.416; 35: 0.421; 36: 0.271; 38: 31.25; 39: 0.289; 40: 0.281; 41: 0.285; 42: 0.135; 44: 15.6; 45: 0.227; 46: 0.224; 47: 0.226; 48: 0.076; 50: 0; 51: 0.151; 52: 0.149; 53: 0.150; 1: pg/ml; 2: OD1; 3: OD2; 4: Average; 5: Corrected; 1: 1000; 2: 2.795; 3: 2.598; 4: 2.697; 5: 2.547; 1: 500; 2: 2.320; 3: 2.189; 4: 2.255; 5: 2.105; 1: 250; 2: 1.644; 3: 1.564; 4: 1.604; 5: 1.454; 1: 125; 2: 0.913; 3: 0.885; 4: 0.899; 5: 0.749; 1: 62.5; 2: 0.425; 3: 0.416; 4: 0.421; 5: 0.271; 1: 31.25; 2: 0.289; 3: 0.281; 4: 0.285; 5: 0.135; 1: 15.6; 2: 0.227; 3: 0.224; 4: 0.226; 5: 0.076; 1: 0; 2: 0.151; 3: 0.149; 4: 0.150 |
| ELISA Data Analysis | ELISA Standard Curve & Curve Expert software |
| Shelf Life | 6 months |
| Description | This Human VIP ELISA Kit was designed for the quantitative measurement of Human VIP protein in serum, plasma, tissue homogenates. It is a Sandwich ELISA kit, its detection range is 15.6 pg/mL-1000 pg/mL and the sensitivity is 3.9 pg/mL. |
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