Human adiponectin,ADP ELISA Kit CSB-E07270h CUSABIO
$50.00
In stock
SKU
CSB-E07270h
Product Details
| Target Name | adiponectin, C1Q and collagen domain containing |
| Uniprot No. | Q15848 |
| Species | Homo sapiens (Human) |
| Storage | Store at 2-8°C. Please refer to protocol. |
| Sensitivity | 1.102 ng/mL |
| Troubleshooting and FAQs | ELISA kit FAQs |
| Lead Time | 3-5 working days after you place the order, and it takes another 3-5 days for delivery via DHL or FedEx. |
| Associated names | 30 kDa adipocyte complement related protein ELISA Kit; 30 kDa adipocyte complement-related protein ELISA Kit; ACDC ELISA Kit; Acrp 30 ELISA Kit; ACRP30 ELISA Kit; ADIPO_HUMAN ELISA Kit; Adipocyte ELISA Kit; Adipocyte C1q and collagen domain containing protein ELISA Kit; Adipocyte complement related 30 kDa protein ELISA Kit; Adipocyte complement related protein of 30 kDa ELISA Kit; Adipocyte complement-related 30 kDa protein ELISA Kit; adipocyte-specific secretory protein ELISA Kit; Adiponectin ELISA Kit; Adiponectin precursor ELISA Kit; adiponectin; C1Q and collagen domain containing ELISA Kit; Adipoq ELISA Kit; Adipose most abundant gene transcript 1 ELISA Kit; Adipose most abundant gene transcript 1 protein ELISA Kit; Adipose specific collagen like factor ELISA Kit; ADIPQTL1 ELISA Kit; ADPN ELISA Kit; APM 1 ELISA Kit; apM-1 ELISA Kit; APM1 ELISA Kit; C1q and collagen domain-containing protein ELISA Kit; GBP 28 ELISA Kit; GBP28 ELISA Kit; Gelatin binding protein ELISA Kit; Gelatin binding protein 28 ELISA Kit; gelatin-binding protein 28 ELISA Kit; Gelatin-binding protein ELISA Kit; OTTHUMP00000210047 ELISA Kit |
| Abbreviation | ADP |
| Sample Types | serum, plasma, cell culture supernates, tissue homogenates, urine |
| Detection Range | 1.562 ng/mL-100 ng/mL |
| Assay Time | 1-5h |
| Sample Loading Volume | 50-100ul |
| Detection Wavelength | 450 nm |
| Research Area | Cardiovascular |
| Assay Principle | quantitative |
| Measurement | Sandwich |
| Precision | Intra-assay Precision (Precision within an assay): CV%<8% Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision (Precision between assays): CV%<10% Three samples of known concentration were tested in twenty assays to assess. |
| Linearity | To assess the linearity of the assay, samples were spiked with high concentrations of human ADP in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. |
| Typical Data | These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. |
| Materials provided | A micro ELISA plate --- The 96-well plate has been pre-coated with an anti-human ADP antibody. This dismountable microplate can be divided into 12 x 8 strip plates. Two vials lyophilized standard --- Dilute a bottle of the standard at dilution series, read the OD values, and then draw a standard curve. One vial Biotin-labeled ADP antibody (100 x concentrate) (120 μl/bottle) ---Act as the detection antibody. One vial HRP-avidin (100 x concentrate) (120 μl/bottle) --- Bind to the detection antibody and react with the TMB substrate to make the solution chromogenic. One vial Biotin-antibody Diluent (15 ml/bottle) ---Dilute the high concentration Biotin-antibody to an appropriate working solution. One vial HRP-avidin Diluent (15 ml/bottle) ---Dilute the high concentration HRP-avidin solution to an appropriate solution. One vial Sample Diluent (50 ml/bottle)---Dilute the sample to an appropriate concentration. One vial Wash Buffer (25 x concentrate) (20 ml/bottle) --- Wash away unbound or free substances. One vial TMB Substrate (10 ml/bottle) --- Act as the chromogenic agent. TMB interacts with HRP, eliciting the solution turns blue. One vial Stop Solution (10 ml/bottle) --- Stop the color reaction. The solution color immediately turns from blue to yellow. Four Adhesive Strips (For 96 wells) --- Cover the microplate when incubation. An instruction manual |
| Materials not provided | A microplate reader capable of measuring absorbance at 450 nm, with the correction wavelength set at 540 nm or 570 nm. An incubator can provide stable incubation conditions up to 37°C±5°C. Centrifuge Vortex Squirt bottle, manifold dispenser, or automated microplate washer Absorbent paper for blotting the microtiter plate 50-300ul multi-channel micropipette Pipette tips Single-channel micropipette with different ranges 100ml and 500ml graduated cylinders Deionized or distilled water Timer Test tubes for dilution |
| ELISA Data Analysis | ELISA Standard Curve & Curve Expert software |
| Shelf Life | 6 months |
| Description | This Human ADP ELISA Kit was designed for the quantitative measurement of Human ADP protein in serum, plasma, cell culture supernates, tissue homogenates, urine. It is a Sandwich ELISA kit, its detection range is 1.562 ng/mL-100 ng/mL and the sensitivity is 1.102 ng/mL. |
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