Human anti-cyclic citrullinated peptide antibody (anti-CCP antibody) ELISA Kit CSB-E09077h CUSABIO
$50.00
In stock
SKU
CSB-E09077h
Product Details
| Target Name | anti-cyclic citrullinated peptide antibody (anti-CCP antibody) |
| Species | Homo sapiens (Human) |
| Storage | Store at 2-8°C. Please refer to protocol. |
| Sensitivity | Request Information |
| Troubleshooting and FAQs | ELISA kit FAQs |
| Lead Time | 3-5 working days after you place the order, and it takes another 3-5 days for delivery via DHL or FedEx. |
| Abbreviation | ACCPA |
| Sample Types | serum |
| Detection Range | Request Information |
| Assay Time | 1-5h |
| Sample Loading Volume | 50-100ul |
| Detection Wavelength | 450 nm |
| Research Area | Autoimmunity |
| Precision | headers: Intra-assay Precision (Precision within an assay): CV%<15%; rows: Intra-assay Precision (Precision within an assay): CV%<15%: Intra-assay Precision (Precision within an assay): CV%<15%; 1: Three samples of known concentration were tested twenty times on one plate to assess.; Intra-assay Precision (Precision within an assay): CV%<15%: Inter-assay Precision (Precision between assays): CV%<15%; 1: Three samples of known concentration were tested in twenty assays to assess. |
| Typical Data | headers: Test parameter; specification; test result; rows: Test parameter: Test parameter; specification: specification; test result: test result; Test parameter: Positive control; specification: ≥0.8; test result: 2.078; Test parameter: Negative control; specification: ≤0.1; test result: 0.075; Test parameter: Positive rate; specification: 10，Positive; test result: 100%; Test parameter: Negative rate; specification: 10，Negative; test result: 100% |
| Materials provided | A 96-well Assay plate --The 96-well plate has been pre-coated with CCP antigen. Negative Control (1 x 0.8 ml) Positive Control (1 x 0.8 ml) HRP-conjugated IgG antibody(1 x 10m)l --Bind to the human anti-CCP antibody, and HRP catalyzes the TMB to elicit a chromogenic reaction. Sample Diluent (2 x 20 ml) --Dilute the sample to an appropriate concentration. Wash Buffer (25x concentrate) (1 x 20 ml) --Wash away unbound or free substances. Substrate A (1 x 5 ml) --Mix with substrate B and interact with HRP, eliciting a Chromogenic reaction. Substrate B (1 x 5 ml) --Mix with substrate A and interact with HRP, eliciting a Chromogenic reaction. Stop Solution (1 x 5ml) --Stop the color reaction. The solution color immediately turns from blue to yellow. Four Adhesive Strips (For 96 wells) An Instruction manual |
| Materials not provided | A microplate reader capable of measuring absorbance at 450 nm, with the correction wavelength set at 600 nm - 630 nm. An incubator that can provide stable incubation conditions up to 37°C±5°C. Centrifuge Vortex Squirt bottle, manifold dispenser, or automated microplate washer Absorbent paper for blotting the microtiter plate 50-300ul multi-channel micropipette Pipette tips Single-channel micropipette with different ranges 100ml and 500ml graduated cylinders Deionized or distilled water Timer Test tubes for dilution |
| Shelf Life | 6 months |
| Description | Anti-cyclic citrullinated peptide antibodies are autoantibodies that recognize citrullinated proteins, which form through post-translational modification of arginine residues by peptidylarginine deiminase enzymes. These antibodies serve as highly specific biomarkers for rheumatoid arthritis and appear in approximately 60-70% of patients with this autoimmune condition. Anti-CCP antibodies can emerge years before clinical symptoms develop and correlate with more severe joint damage and erosive disease progression, making them valuable for early diagnosis and prognosis. This assay operates at a detection wavelength of 450 nm and requires 50-100 μl sample volume per test. The complete assay procedure takes 1-5 hours, providing researchers with an efficient method for measuring these clinically relevant autoantibodies in human specimens. Application Examples Note: The following application examples are drawn from a selection of publications citing this product. For additional applications, please refer to the full list of references in the "Citations" section. This ELISA kit has been applied in clinical research studies focused on rheumatic and autoimmune diseases, particularly for measuring anti-cyclic citrullinated peptide antibody levels in serum samples. The kit supports diagnostic profiling alongside other inflammatory and immunological markers in patient populations. • Rheumatic disease research: Applied in studies examining rheumatoid arthritis, reactive arthritis, ankylosing spondylitis, and psoriatic arthritis through multi-laboratory collaborative research efforts • Autoimmune disorder profiling: Used for diagnostic marker evaluation in systemic lupus erythematosus and Sjögren's syndrome patient cohorts compared to control groups • Clinical biomarker panels: Integrated into laboratory diagnostic workflows measuring inflammatory markers, complement proteins, and immunoglobulins for rheumatology applications • Multi-center diagnostic studies: Used across independent clinical laboratories and hospital settings for standardized autoimmune disease classification and patient categorization |
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